Platelet adhesion to human umbilical vein endothelial cells cultured on anionic hydrogel scaffolds.

In this work we describe experiments designed to understand the human platelet adhesion to human umbilical vein endothelial cells (HUVECs) cultured on various kinds of chemically cross-linked anionic hydrogels, which were synthesized by radical polymerization. HUVECs could proliferate to sub-confluent or confluent on poly(acrylic acid) (PAA), poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS), and poly(sodium p-styrene sulfonate) (PNaSS) gels. The proliferation behavior was not sensitive to the cross-linker concentration of the gels. However, the platelet adhesion on the HUVECs cultured on these gels showed different behavior, as revealed by human platelet adhesion test in static conditions. Only a few platelets adhered on the HUVEC sheets cultured on PNaAMPS gels with 4 and 10mol% cross-linker concentrations, and completely no platelet adhered on the HUVEC sheets cultured on PNaSS gels with 4 and 10mol% cross-linker concentrations. On the other hand, a large number of platelets adhered on the HUVECs cultured on PAA gels with 1, 2mol% cross-linker concentrations and PNaAMPS gel with 2mol% cross-linker concentration. Furthermore, the study showed that promote of the glycocalyx of HUVECs with transforming growth factor-beta(1) (TGF-beta(1)) decreased platelet adhesion, and degrade the glycocalyx with heparinase I increased platelet adhesion. The results suggested that the glycocalyx of cultured HUVECs modulates platelet compatibility, and the amount of glycocalyx secreted by HUVECs dependents on the chemical structure and cross-linker concentration of gel scaffolds. This result should be applied to make the hybrid artificial blood vessel composes of gels and endothelial cells with high platelet compatibility.

[1]  Hans Vink,et al.  The Endothelial Glycocalyx Protects Against Myocardial Edema , 2003, Circulation research.

[2]  Akira Mochizuki,et al.  Effect of water structure on blood compatibility--thermal analysis of water in poly(meth)acrylate. , 2004, Journal of biomedical materials research. Part A.

[3]  A. Mochizuki,et al.  Blood compatible aspects of poly(2-methoxyethylacrylate) (PMEA)--relationship between protein adsorption and platelet adhesion on PMEA surface. , 2000, Biomaterials.

[4]  Yoshihito Osada,et al.  Cultivation of endothelial cells on adhesive protein-free synthetic polymer gels. , 2005, Biomaterials.

[5]  B. Strauss,et al.  The role of perlecan in arterial injury and angiogenesis. , 2004, Cardiovascular research.

[6]  J. M. Harris,et al.  Poly(Ethylene Glycol) Chemistry Biotechnical and Biomedical Applications , 1992 .

[7]  Y Noishiki,et al.  A new antithrombogenic material with long polyethyleneoxide chains. , 1982, Transactions - American Society for Artificial Internal Organs.

[8]  R. Linhardt,et al.  Examination of the substrate specificity of heparin and heparan sulfate lyases. , 1990, Biochemistry.

[9]  P. Serruys,et al.  Influence of Coronary Vessel Size on Renarrowing Process and Late Angiographic Outcome After Successful Balloon Angioplast , 1994, Circulation.

[10]  V. Hascall,et al.  Cell surface heparan sulfate proteoglycans. , 1992, The Journal of biological chemistry.

[11]  A. Hjerpe,et al.  Immunoreactivity to cell surface syndecans in cytoplasm and nucleus: tubulin-dependent rearrangements. , 2002, Experimental cell research.

[12]  Y. Ikada,et al.  Polyurethane surface modification by graft polymerization of acrylamide for reduced protein adsorption and platelet adhesion. , 1993, Biomaterials.

[13]  D. Spillmann,et al.  Structural Requirements and Mechanism for Heparin-induced Activation of a Recombinant Mouse Mast Cell Tryptase, Mouse Mast Cell Protease-6 , 2001, The Journal of Biological Chemistry.

[14]  P. Losi,et al.  Luminal surface microgeometry affects platelet adhesion in small-diameter synthetic grafts. , 2004, Biomaterials.

[15]  T. Tsuruta.  Contemporary Topics in Polymeric Materials for Biomedical Applications , 1996 .

[16]  A. Cheung,et al.  The effects of heparin, protamine, and heparinase 1 on platelets in vitro using whole blood flow cytometry. , 2000, Anesthesia and analgesia.

[17]  D. Mooney,et al.  Hydrogels for tissue engineering. , 2001, Chemical Reviews.

[18]  N. B. Graham,et al.  Interaction of poly(ethylene oxide) with solvents: 2. Water-poly(ethylene glycol) , 1989 .

[19]  C. Tian,et al.  Blood compatibility of surface-engineered poly(ethylene terephthalate) via o-carboxymethylchitosan. , 2006, Colloids and surfaces. B, Biointerfaces.

[20]  A. Mochizuki,et al.  Study of blood compatibility with poly(2-methoxyethyl acrylate). Relationship between water structure and platelet compatibility in poly(2-methoxyethylacrylate-co-2-hydroxyethylmethacrylate). , 2002, Biomacromolecules.

[21]  E. Edelman,et al.  Perlecan is required to inhibit thrombosis after deep vascular injury and contributes to endothelial cell-mediated inhibition of intimal hyperplasia. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[22]  T. Wight,et al.  Cell Density-dependent Regulation of Proteoglycan Synthesis by Transforming Growth Factor-β1 in Cultured Bovine Aortic Endothelial Cells* , 2000, The Journal of Biological Chemistry.

[23]  Young Baek Kim,et al.  Poly(ethylene glycol)-grafted poly(3-hydroxyundecenoate) networks for enhanced blood compatibility. , 2003, International journal of biological macromolecules.

[24]  J. Spaan,et al.  Oxidized lipoproteins degrade the endothelial surface layer : implications for platelet-endothelial cell adhesion. , 2000, Circulation.

[25]  Hyoe Hatakeyama,et al.  Interaction between water and hydrophilic polymers , 1998 .

[26]  Allan S Hoffman,et al.  Hydrogels for biomedical applications. , 2002, Advanced drug delivery reviews.

[27]  Randy S. Lewis,et al.  Improved hemocompatibility of poly(ethylene terephthalate) modified with various thiol-containing groups. , 2005, Biomaterials.

[28]  F. Pavalko,et al.  Improved biocompatibility of small intestinal submucosa (SIS) following conditioning by human endothelial cells. , 2004, Biomaterials.

[29]  Jiang Yuan,et al.  Platelet adhesive resistance of segmented polyurethane film surface-grafted with vinyl benzyl sulfo monomer of ammonium zwitterions. , 2003, Biomaterials.

[30]  Jeffry A Florian,et al.  Heparan Sulfate Proteoglycan Is a Mechanosensor on Endothelial Cells , 2003, Circulation research.

[31]  J. Ratto,et al.  Differential scanning calorimetry investigation of phase transitions in water/ chitosan systems , 1995 .

[32]  B. Kasinath.  Glomerular Endothelial Cell Proteoglycans - Regulation by TGF-β1 , 1993 .

[33]  J. Weitz,et al.  Heparan sulfate: antithrombotic or not? , 2003, The Journal of clinical investigation.

[34]  Axel R Pries,et al.  Microvascular blood flow resistance: role of endothelial surface layer. , 1997, American journal of physiology. Heart and circulatory physiology.

[35]  J. J. Schwartz,et al.  Normal levels of anticoagulant heparan sulfate are not essential for normal hemostasis. , 2003, The Journal of clinical investigation.

[36]  Y. Bae,et al.  PDMS-based polyurethanes with MPEG grafts: synthesis, characterization and platelet adhesion study. , 1999, Biomaterials.

[37]  A. Mochizuki,et al.  Cold crystallization of water in hydrated poly(2-methoxyethyl acrylate) (PMEA) , 2000 .

[38]  M. Karnovsky,et al.  Inhibition of Rat Arterial Smooth Muscle Cell Proliferation by Heparin: In Vivo Studies with Anticoagulant and Nonanticoagulant Heparin , 1980, Circulation research.

[39]  A. Poot,et al.  Platelet deposition studies on copolyether urethanes modified with poly(ethylene oxide). , 1990, Biomaterials.

[40]  Y. Bae,et al.  Hydrogels based on poly(ethylene oxide) and poly(tetramethylene oxide) or poly(dimethyl siloxane): synthesis, characterization, in vitro protein adsorption and platelet adhesion. , 2002, Biomaterials.

[41]  T. Okano,et al.  Effect of hydrophilic and hydrophobic microdomains on mode of interaction between block polymer and blood platelets. , 1981, Journal of biomedical materials research.

[42]  A. Mulivor,et al.  Role of glycocalyx in leukocyte-endothelial cell adhesion. , 2002, American journal of physiology. Heart and circulatory physiology.

[43]  K. Nishinari,et al.  Effects of polyols and sugars on the structure of water in concentrated gelatin gels as studied by low temperature differential scanning calorimetry , 1997 .

[44]  J. H. Lee,et al.  Platelet adhesion onto chargeable functional group gradient surfaces. , 1998, Journal of biomedical materials research.