High resolution in vivo micro-CT with flat panel detector based on amorphous silicon.

A high-resolution in vivo micro-CT system for combining with fluorescence molecular tomography (FMT), was constructed and applied in small animal imaging. The fast scanning micro-CT system designed to provide high-resolution anatomic information and reconstruction priors, consisted of a flat panel detector (FPD) based on amorphous silicon (a-Si) and a micro-focus x-ray tube. The Feldkamp algorithm was adopted in image reconstruction with graphic processing unit (GPU). The system spatial resolution of 13 lp/mm was achieved when the diameter of image field was 6 cm with the system magnification factor of 4. No obvious beam hardening artifact was observed in transaxial image of a water phantom after correction. The contrast-to-noise ratio (CNR) study of various tissue phantoms was also presented. The in-vivo imaging of an anesthetic mouse was performed to demonstrate the feasibility of our system.

[1]  Ehsan Samei.  Image quality in two phosphor-based flat panel digital radiographic detectors. , 2003, Medical physics.

[2]  Lingyun Chen,et al.  Spatial resolution properties in cone beam CT: a simulation study. , 2008, Medical physics.

[3]  Gyuseong Cho,et al.  Use of a flat-panel detector for microtomography: a feasibility study for small-animal imaging , 2005 .

[4]  M. Desco,et al.  Assessment of a New High-Performance Small-Animal X-Ray Tomograph , 2008, IEEE Transactions on Nuclear Science.

[5]  R Weissleder,et al.  Molecular imaging. , 2009, Radiology.

[6]  J. Boone,et al.  Evaluation of the spatial resolution characteristics of a cone-beam breast CT scanner. , 2006, Medical physics.

[7]  Min Hyoung Cho,et al.  A flat-panel detector based micro-CT system: performance evaluation for small-animal imaging. , 2003, Physics in medicine and biology.

[8]  F Verhaegen,et al.  SpekCalc: a program to calculate photon spectra from tungsten anode x-ray tubes , 2009, Physics in medicine and biology.

[9]  G. Johnson,et al.  In vivo small-animal imaging using micro-CT and digital subtraction angiography , 2008, Physics in medicine and biology.

[10]  Simon R Cherry,et al.  Simultaneous molecular and anatomical imaging of the mouse in vivo , 2002, Physics in medicine and biology.

[11]  Guotao Quan,et al.  FAST RECONSTRUCTION METHOD BASED ON COMMON UNIFIED DEVICE ARCHITECTURE (CUDA) FOR MICRO-CT , 2010 .

[12]  Stephen B. Tuttle,et al.  Magnetic resonance-coupled fluorescence tomography scanner for molecular imaging of tissue. , 2008, The Review of scientific instruments.

[13]  Shaun S. Gleason,et al.  A new X-ray computed tomography system for laboratory mouse imaging , 1998 .

[14]  S R Cherry,et al.  A microPET/CT system for in vivo small animal imaging. , 2007, Physics in medicine and biology.

[15]  Vasilis Ntziachristos,et al.  Tomographic fluorescence imaging of tumor vascular volume in mice. , 2007, Radiology.

[16]  Ehsan Samei,et al.  Assessment of detective quantum efficiency: intercomparison of a recently introduced international standard with prior methods. , 2007, Radiology.

[17]  E. Samei,et al.  A method for measuring the presampled MTF of digital radiographic systems using an edge test device. , 1998, Medical physics.

[18]  A Fenster,et al.  A high-resolution XRII-based quantitative volume CT scanner. , 1993, Medical physics.

[19]  Bryan Hoang,et al.  Noninvasive monitoring of the fate of 111In-labeled block copolymer micelles by high resolution and high sensitivity microSPECT/CT imaging. , 2009, Molecular pharmaceutics.

[20]  Sol M. Grunera.  Charge-coupled device area x-ray detectors , 2002 .

[21]  I A Cunningham,et al.  Signal and noise in modulation transfer function determinations using the slit, wire, and edge techniques. , 1992, Medical physics.

[22]  W. Kalender,et al.  Empirical cupping correction: a first-order raw data precorrection for cone-beam computed tomography. , 2006, Medical physics.

[23]  Willi A Kalender,et al.  Geometric misalignment and calibration in cone-beam tomography. , 2004, Medical physics.

[24]  J A Rowlands,et al.  X-ray detectors for digital radiography. , 1997, Physics in medicine and biology.

[25]  Xiaoquan Yang,et al.  Fluorescence molecular tomography system for in vivo tumor imaging in small animals , 2010 .

[26]  V. Ntziachristos,et al.  Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion. , 2007, Medical physics.

[27]  Kai Yang,et al.  A geometric calibration method for cone beam CT systems. , 2006, Medical physics.

[28]  J H Siewerdsen,et al.  Cone-beam computed tomography with a flat-panel imager: initial performance characterization. , 2000, Medical physics.

[29]  Andre Obenaus,et al.  Radiation dose in rodent tissues during micro-CT imaging , 2004 .

[30]  M J Paulus,et al.  High resolution X-ray computed tomography: an emerging tool for small animal cancer research. , 2000, Neoplasia.

[31]  Martin G Pomper,et al.  Small animal imaging in drug development. , 2005, Current pharmaceutical design.

[32]  Vasilis Ntziachristos,et al.  Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo , 2008, Proceedings of the National Academy of Sciences.

[33]  Qingming Luo,et al.  Combined system of fluorescence diffuse optical tomography and microcomputed tomography for small animal imaging. , 2010, The Review of scientific instruments.

[34]  Ruola Ning,et al.  Flat panel detector-based cone-beam volume CT angiography imaging: system evaluation , 2000, IEEE Transactions on Medical Imaging.

[35]  L. Feldkamp,et al.  Practical cone-beam algorithm , 1984 .

[36]  J. Culver,et al.  Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice. , 2005, Optics express.