Partial primary structure of the alpha and beta chains of human tumor T-cell receptors.

The T-cell receptor for antigen (Ti) was purified from the human tumor cell line HPB-ALL. Amino-terminal sequence analysis of an acid-cleaved peptide of the Ti alpha chain showed that it is highly homologous to a putative murine alpha chain recently described. Amino-terminal sequence analysis of the Ti beta chain revealed that it shares 50 percent homology with the Ti beta chain amino acid sequences from two other human T-cell tumors. Nucleotide sequence analysis of a complementary DNA clone encoding the Ti beta chain from the HPB-MLT cell line showed that this chain represents a second human constant region gene segment and suggested that it arises from direct joining of the variable and joining gene segments without any intervening D region sequences.

[1]  J. Freed,et al.  Immunoglobulin-like nature of the α-chain of a human T-cell antigen/MHC receptor , 1984, Nature.

[2]  Mark M. Davis,et al.  A third type of murine T-cell receptor gene , 1984, Nature.

[3]  S. Tonegawa,et al.  A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes , 1984, Nature.

[4]  L. Hood,et al.  The structure, rearrangement and expression of Dβ gene segments of the murine T-cell antigen receptor , 1984, Nature.

[5]  Mark M. Davis,et al.  Genomic organization and sequence of T-cell receptor β-chain constant- and joining-region genes , 1984, Nature.

[6]  Tak W. Mak,et al.  Mouse T cell antigen receptor: Structure and organization of constant and joining gene segments encoding the β polypeptide , 1984, Cell.

[7]  S. Tonegawa,et al.  Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences , 1984, Nature.

[8]  Mark M. Davis,et al.  Somatic recombination in a murine T-cell receptor gene , 1984, Nature.

[9]  Mark M. Davis,et al.  Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins , 1984, Nature.

[10]  Mark M. Davis,et al.  Isolation of cDNA clones encoding T cell-specific membrane-associated proteins , 1984, Nature.

[11]  Tak W. Mak,et al.  A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains , 1984, Nature.

[12]  P. Marrack,et al.  The major histocompatibility complex-restricted antigen receptor on T cells in mouse and man: Identification of constant and variable peptides , 1983, Cell.

[13]  P. Marrack,et al.  The mouse T cell receptor: Comparison of MHC-restricted receptors on two T cell hybridomas , 1983, Cell.

[14]  S. Tonegawa.  Somatic generation of antibody diversity , 1983, Nature.

[15]  E. Reinherz,et al.  Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex , 1983, The Journal of experimental medicine.

[16]  L. Hood,et al.  Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. , 1983, Methods in enzymology.

[17]  J. Allison,et al.  Tumor-specific antigen of murine T-lymphoma defined with monoclonal antibody. , 1982, Journal of immunology.

[18]  J. Morrissey,et al.  Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. , 1981, Analytical biochemistry.

[19]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[20]  J. Strominger,et al.  Further structural studies of the heavy chain of HLA antigens and its similarity to immunoglobulins. , 1977, Proceedings of the National Academy of Sciences of the United States of America.