Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae

THE wide host range of plasmids of the P-incompatibility class1,2 has attracted interest in this group of R factors. A 3×106-dalton sequence which includes the TEM β-lactamase gene found on some plasmids of this group can integrate into the Escherichia coli chromosome3 and into plasmids of other compatibility groups1,4,5. This translocatable sequence has been termed transposon A (TnA)4,6 and resembles the insertion sequences (IS) of E. coli, in that TnA insertions can result in gene inactivation, giving rise to strong polar mutations7.

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