Adventitious Shoot Regeneration and Rooting of Prunus serotina In Vitro Cultures

A complete regeneration protocol was developed for Prunus serotina Ehrh., an important hardwood species for timber and sawlog production in the central and eastern United States. Nodal sections were cultures on Murashige and Skoog (MS) medium supplemented with 4.44 µM 6-benzylaminopurine (BA), 0.49 µM indole-3-butyric acid (IBA), and 0.29 µM gibberellic acid (GA3). In vitro leaf explants of three genotypes were placed on woody plant medium (WPM) supplemented with 0, 2.27, 4.54, or 6.81 µM thidiazuron (TDZ) in combination with 0, 0.54, 1.07, or 5.37 µM naphthaleneacetic acid (NAA), and on WPM supplemented with 0, 4.44, 8.88, or 13.32 µM BA in combination with 0, 0.54, 1.07, or 5.37 µM NAA. Cultures were maintained either in continuous darkness for 5 weeks, or in the dark for 3 weeks and then transferred to a 16-hour photoperiod. TDZ and the genotype had a significant effect on the number of shoots regenerated. The maximum mean number of shoots regenerated per explant (5.05 +/- 1.14) was obtained with 2.27 µM TDZ plus 0.54 µM NAA with the 3-week dark period then light treatment. The highest percent shoot regeneration (38.3) and the mean number of shoots (4.13 +/- 0.97) was obtained with 6.81 µM TDZ plus 1.07 µM NAA. The highest rooting (27%) of adventitious shoots and number of roots per shoot (2.3 +/- 0.2) was obtained with 2.5 µM IBA when shoots were maintained for 7 days in the dark on rooting medium before transfer to a 16-hour photoperiod. The highest rooting (70%) of nodal explant-derived stock cultures and number of roots per shoot (2.7 +/- 0.9) was also obtained with 2.5 µM IBA, but when shoots were maintained for 4 days in the dark before transfer to a 16-hour photoperiod. In total, 86% of the plantlets survived acclimitization to the greenhouse and 100% survival after overwintering in cold-storage.

[1]  M. Oliveira,et al.  Shoot regeneration from adventitious buds induced on juvenile and adult almond (Prunus dulcis mill.) explants , 1996, In Vitro Cellular & Developmental Biology - Plant.

[2]  C. Maynard Six-year field test results of micropropagated black cherry (Prunus serotina) , 2007, In Vitro Cellular & Developmental Biology - Plant.

[3]  W. Lane,et al.  In vitro Shoot Regeneration from Leaves of Sweet Cherry (Prunus avium) 'Lapins' and 'Sweetheart' , 2004, Plant Cell, Tissue and Organ Culture.

[4]  B. Nowak,et al.  Sugar Uptake and Utilisation during Adventitious Bud Differentiation on in Vitro Leaf Explants of ‘Wegierka Zwykła’ Plum (Prunus Domestica) , 2004, Plant Cell, Tissue and Organ Culture.

[5]  M. Sedgley,et al.  Regeneration of almond from immature seed cotyledons , 2001, Plant Cell, Tissue and Organ Culture.

[6]  Neil Hammatt,et al.  Adventitious shoot development from wild cherry (Prunus avium L.) leaves , 2000, New Forests.

[7]  L. Burgos,et al.  Different media requirements for micropropagation of apricot cultivars , 2000, Plant Cell, Tissue and Organ Culture.

[8]  T. Astatkie,et al.  Micropropagation of Chokecherry and Pincherry cultivars , 2000, Plant Cell, Tissue and Organ Culture.

[9]  AL-Sabbagh Muna,et al.  In vitro propagation of a semi-dwarfing cherry rootstock , 1999, Plant Cell, Tissue and Organ Culture.

[10]  C. Beyl,et al.  In vitro responses and acclimatization of Prunus serotina with paclobutrazol , 1994, Journal of Plant Growth Regulation.

[11]  M. Sedgley,et al.  Micropropagation of almond cultivars Nonpareil and Ne Plus Ultra and the hybrid rootstock Titan×Nemaguard , 2003 .

[12]  L. Burgos,et al.  Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves , 2003, Plant Cell Reports.

[13]  C. Damiano,et al.  Adventitious shoot regeneration in peach [Prunus persica (L.) Batsch] , 2002, Plant Cell Reports.

[14]  M. Sedgley,et al.  In vitro rooting of almond (Prunus dulcis Mill.) , 2001, In Vitro Cellular & Developmental Biology - Plant.

[15]  M. Sedgley,et al.  Adventitious shoot regeneration from leaf explants of almond (Prunus dulcis mill.) , 2000, In Vitro Cellular & Developmental Biology - Plant.

[16]  Egea,et al.  Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot. , 2000, Plant science : an international journal of experimental plant biology.

[17]  Haoru Tang,et al.  Somatic embryogenesis and organogenesis from immature embryo cotyledons of three sour cherry cultivars (Prunus cerasus L.) , 2000 .

[18]  Grant,et al.  Increased root and shoot production during micropropagation of cherry and apple rootstalks: effect of subculture frequency. , 1999, Tree physiology.

[19]  S. Strauss,et al.  Genetic Engineering of Sexual Sterility in Shade Trees , 1998, Arboriculture & Urban Forestry.

[20]  Neil Hammatt,et al.  Shoot regeneration from leaves of Prunus serotina Ehrh. (black cherry) and P. avium L. (wild cherry) , 1998, Plant Cell Reports.

[21]  M. M. Fouad,et al.  FACTORS INFLUENCING ROOTING OF PEACH SHOOTS CULTURED IN VITRO , 1995 .

[22]  R. Scorza,et al.  Regeneration of peach [Prunus persica (L.) Batsch] rootstock cultivars from cotyledons of mature stored seed , 1995 .

[23]  K. Kavanagh,et al.  The effects of GA3 and organic solvents on acclimatization of tissue culture propagated black cherry (Prunus serotina Ehrh.) plantlets , 1993 .

[24]  Neil Hammatt Micropropagation of fastigiate bird cherry (Prunus padus L.) and adventitious shoot formation from leaves , 1993 .

[25]  G. Bassi,et al.  IN VITRO SHOOT REGENERATION OF "BLUEFRE" AND "SUSINA DI DRO" PRUNE CULTIVARS (PRUNUS DOMESTICA L.) (1) , 1991 .

[26]  K. Kavanagh,et al.  Black Cherry (Prunus serotina Ehrh.) , 1991 .

[27]  C. Maynard,et al.  Light effects on in vitro adventitious root formation in axillary shoots of mature Prunus serotina , 1990 .

[28]  P. Druart,et al.  The use of a brief 2,4-D treatment to induce leaf regeneration on Prunus canescens Bois. , 1990 .

[29]  H. Katinger,et al.  REGENERATION OF SHOOTS FROM LEAF DISCS AND STEM MICROCUTTINGS OF FRUIT TREES AS A TOOL FOR TRANSFORMATION , 1989 .

[30]  M. Antonelli,et al.  IN VITRO ROOTING OF DIFFERENT PEACH GENOTYPES , 1988 .

[31]  D. Kester,et al.  Micropropagation of Cherry Rootstocks: II. Invigoration and Enhanced Rooting of ‘46-1 Mazzard’ by Co-culture with ‘Colt’ , 1988, Journal of the American Society for Horticultural Science.

[32]  S. Morini,et al.  In Vitro Propagation of P.S. B2 Peach Rootstock. , 1985 .

[33]  W. Feucht,et al.  MICROPROPAGATION OF PRUNUS CERASUS ROOTSTOCKS - INFLUENCE OF CULTURE MEDIUM CONSTITUENTS ON GROWTH IN STAGE I AND II , 1985 .

[34]  B. Borkowska MICROPROPAGATION OF SOUR CHERRY, CULTIVAR SCHATTENMORELLE , 1985 .

[35]  J. Baumgras,et al.  Distribution of Gum Spots by Causal Agent in Black Cherry and Effects On Log and Tree Quality , 1984 .

[36]  B. McCown,et al.  Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture , 1980 .

[37]  P. Mehra,et al.  Organogenesis and Plantlet Formation In vitro in Almond , 1974, Botanical Gazette.

[38]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .