Paper-based electrochemiluminescence biosensor for cancer cell detection

Abstract Here a novel paper-based electrochemiluminescence (ECL) device was developed for the detection of HL-60 cancer cells. In this approach, porous filter paper as a thin-layer electrochemical cell connected to a sheet of ITO working electrode. Au nanoparticles (Au NPs) and graphene were coated on the surface of ITO to immobilize capture HS-DNA. Then, the aptamer of HL-60 cancer cells, which is labeled with Ru(bpy)32 +-conjugated silica nanoparticles (RuSi@Ru(bpy)32 +) is hybridized with the capture DNA to form bioconjugates. In the presence of HL-60 cells, the aptamer would conjugate with glycoprotein at cell surface and RuSi@Ru(bpy)32 + NP-aptamer would be released. After the dissociation of RuSi@Ru(bpy)32 + NP-aptamer from the surface of ITO, ECL signal would decline. This simple, portable and disposable testing platform could detect HL-60 cancer cells down to 56 cells/mL.

[1]  Jing-Juan Xu,et al.  Sensitive electrochemiluminescence detection of c-Myc mRNA in breast cancer cells on a wireless bipolar electrode. , 2012, Analytical chemistry.

[2]  Audrey K. Ellerbee,et al.  Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper. , 2009, Analytical chemistry.

[3]  G. Whitesides,et al.  Simple telemedicine for developing regions: camera phones and paper-based microfluidic devices for real-time, off-site diagnosis. , 2008, Analytical chemistry.

[4]  Shenguang Ge,et al.  Electrochemical DNA sensor based on three-dimensional folding paper device for specific and sensitive point-of-care testing , 2012 .

[5]  Mamas I. Prodromidis,et al.  Impedimetric immunosensors—A review , 2010 .

[6]  Jinghong Li,et al.  Dynamic evaluation of cell surface N-glycan expression via an electrogenerated chemiluminescence biosensor based on concanavalin A-integrating gold-nanoparticle-modified Ru(bpy)3(2+)-doped silica nanoprobe. , 2013, Analytical chemistry.

[7]  Jing‐Juan Xu,et al.  Ultrasensitive DNA detection based on Au nanoparticles and isothermal circular double-assisted electrochemiluminescence signal amplification. , 2011, Chemical communications.

[8]  Jing-Juan Xu,et al.  Disposable paper-based bipolar electrode for sensitive electrochemiluminescence detection of a cancer biomarker. , 2014, Chemical communications.

[9]  Qin Xu,et al.  A paper disk equipped with graphene/polyaniline/Au nanoparticles/glucose oxidase biocomposite modified screen-printed electrode: toward whole blood glucose determination. , 2014, Biosensors & bioelectronics.

[10]  Chang Lu,et al.  Quantum dot (QD)-modified carbon tape electrodes for reproducible electrochemiluminescence (ECL) emission on a paper-based platform. , 2012, Analytical chemistry.

[11]  Hongyuan Chen,et al.  Electrochemiluminescence resonance energy transfer between CdS:Eu nancrystals and Au nanorods for sensitive DNA detection , 2012 .

[12]  Temsiri Songjaroen,et al.  Blood separation on microfluidic paper-based analytical devices. , 2012, Lab on a chip.