Micro-homology mediated PCR targeting in Saccharomyces cerevisiae.

In the present study, we determined the amount of homology required for targeted integration of DNA fragments into the yeast genome. The procedure described here facilitates the manipulation of the yeast genome and eliminates the need to clone sequences homologous to a target site. In addition, this method is useful for applications in which only limited sequence information of the target is available. The procedure comprises of: (i) production of PCR primers to amplify a selectable marker containing flanking homology to the target of choice; (ii) transformation of yeast cells and (iii) selection of integrants.