The effects of prestorage leukoreduction and storage duration on the in vitro quality of canine packed red blood cells

The effects of prestorage leukoreduction and storage duration on the in vitro quality parameters of canine packed red blood cells (pRBCs) stored in saline-adenine-glucose-mannitol (SAGM) were investigated. Hematological, biochemical, and morphological changes in filtered and unfiltered pRBCs were determined each week during 42 days of storage. All of the parameters changed significantly during storage. The effects of leukoreduction on leukocyte and erythrocyte count, packed cell volume, hemoglobin content, and erythrocyte morphology were significant. While supernatant K increased during storage, glucose, Cl, and 2,3-diphosphoglycerate (2,3-DPG) concentrations decreased. Filtered pRBCs had higher 2,3-DPG levels throughout storage than their unfiltered counterparts. It was concluded that the leukoreduction filter significantly decreases the number of leukocytes and that canine pRBCs can be stored in SAGM for 42 days. Furthermore, leukoreduction may be a consideration with the intention of improving oxygen delivery, since the filtered pRBCs had a higher 2,3-DPG concentration.

[1]  John R Hess,et al.  Red cell changes during storage. , 2010, Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis.

[2]  A. Zubair.  Clinical impact of blood storage lesions , 2009, American journal of hematology.

[3]  G. McGwin,et al.  Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine Blood Transfusion in the Critically Ill: Does Storage Age Matter? , 2022 .

[4]  M. Raekallio,et al.  Storage of equine red blood cells as a concentrate. , 2008, Veterinary journal.

[5]  B. Gathof,et al.  In vitro quality of red blood cells (RBCs) collected by multicomponent apheresis compared to manually collected RBCs during 49 days of storage , 2007, Transfusion.

[6]  J. Hess.  An update on solutions for red cell storage , 2006, Vox sanguinis.

[7]  S. Holme.  Current issues related to the quality of stored RBCs. , 2005, Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis.

[8]  F. Tablin,et al.  Comparison of 4 blood storage methods in a protocol for equine pre-operative autologous donation. , 2004, Veterinary surgery : VS.

[9]  J. Hess,et al.  Storage of red blood cells: new approaches. , 2002, Transfusion medicine reviews.

[10]  W. Reinhart,et al.  Influence of prestorage leucocyte depletion and storage time on rheologic properties of erythrocyte concentrates , 2002, Vox sanguinis.

[11]  J. Jagger,et al.  A comparison of biochemical and functional alterations of rat and human erythrocytes stored in CPDA‐1 for 29 days: implications for animal models of transfusion , 2000, Transfusion medicine.

[12]  M. Štifanić,et al.  Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood , 2000, Vox Sanguinis.

[13]  R. Sellon,et al.  Use of a prestorage leukoreduction filter effectively removes leukocytes from canine whole blood while preserving red blood cell viability. , 2000, Journal of veterinary internal medicine.

[14]  N. Arya,et al.  The red cell storage lesion and its implication for transfusion. , 1997, Transfusion science.

[15]  A. Rapaille,et al.  Prestorage Leukocyte Reduction with In‐Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage , 1997, Vox sanguinis.

[16]  R. Stromberg,et al.  Evaluation of a New Prestorage Leukoreduction Filter for Red Blood Cell Units , 1997, Vox sanguinis.

[17]  R. Tucker,et al.  Evaluation of canine red blood cells stored in a saline, adenine, and glucose solution for 35 days. , 1997, Journal of veterinary internal medicine.

[18]  K. Wardrop.  Selection of anticoagulant-preservatives for canine and feline blood storage. , 1995, The Veterinary clinics of North America. Small animal practice.

[19]  K. Wardrop,et al.  An in vitro evaluation of storage media for the preservation of canine packed red blood cells. , 1994, Veterinary clinical pathology.

[20]  T. J. Owen,et al.  Evaluation of an additive solution for preservation of canine red blood cells. , 1994, Journal of Veterinary Internal Medicine.

[21]  P. J. Armstrong,et al.  Evaluation of citrate-phosphate-dextrose-adenine as a storage medium for packed canine erythrocytes. , 1988, Journal of veterinary internal medicine.

[22]  J. Duhm.  Inosine permeability and purine nucleoside phosphorylase activity as limiting factors for the synthesis of 2,3-diphosphoglycerate from inosine, pyruvate, and inorganic phosphate in erythrocytes of various mammalian species. , 1974, Biochimica et biophysica acta.