Separation of serotonin from catechols by capillary zone electrophoresis with electrochemical detection.

Capillary zone electrophoresis with electrochemical detection is demonstrated with columns having only 9-microns inner diameter. Amperometric detection limits of 0.7 amol are reported for serotonin. The difficult problem of resolving serotonin and dopamine--two neurotransmitters of interest having similar electrophoretic mobilities--is addressed by chemical means to improve selectivity. These include buffer modification with 2-propanol and a system employing borate complexation of the catechol in combination with sodium dodecyl sulfate micelles.

[1]  E. Wang,et al.  A thin-layer electrochemical detector coated with nafion film for liquid chromatography. , 1987, Journal of chromatography.

[2]  A. Ewing,et al.  Capillary zone electrophoresis with electrochemical detection in 12.7 microns diameter columns. , 1988, Analytical chemistry.

[3]  S. Snyder,et al.  Drug and neurotransmitter receptors in the brain. , 1984, Science.

[4]  R. Wightman,et al.  Detection of dopamine dynamics in the brain. , 1988, Analytical chemistry.

[5]  A. Ewing,et al.  Carbon-ring electrodes with 1-.mu.m tip diameter , 1986 .

[6]  A. Ewing,et al.  Characterization of a microinjector for capillary zone electrophoresis , 1987 .

[7]  J. Jorgenson,et al.  Capillary zone electrophoresis. , 1983, Science.

[8]  James W. Jorgenson,et al.  Liquid chromatography in open-tubular columns : Theory of column optimization with limited pressure and analysis time, and fabrication of chemically bonded reversed-phase columns on etched borosilicate glass capillaries , 1983 .

[9]  A. Ewing,et al.  Retention of ionic and non-ionic catechols in capillary zone electrophoresis with micellar solutions. , 1988, Journal of chromatography.

[10]  Koji Otsuka,et al.  Electrokinetic separations with micellar solutions and open-tubular capillaries , 1984 .

[11]  A. Ewing,et al.  Amperometric detection of catechols in capillary zone electrophoresis with normal and micellar solutions. , 1988, Analytical chemistry.

[12]  R. Adams,et al.  Probing brain chemistry with electroanalytical techniques. , 1976, Analytical chemistry.

[13]  Michel Jouvet,et al.  In vivo electrochemical detection of catechols in the neostriatum of anaesthetized rats: dopamine or DOPAC? , 1980, Nature.

[14]  M. Novotny.  Capillary chromatography in the condensed mobile phases: From the concepts to analytically useful separations and measurements , 1987 .

[15]  C. L. Rice,et al.  Electrokinetic Flow in a Narrow Cylindrical Capillary , 1965 .

[16]  S. Honda,et al.  Effect of addition of organic solvent on the separation of positional isomers in high-voltage capillary zone electrophoresis , 1987 .

[17]  L. Tauc,et al.  Micro carbon electrode for intracellular voltammetry , 1986 .