Influence of the physical and chemical properties of magnetic nanoparticles on their performance in a chemiluminescence immunoassay.

OBJECTIVES Magnetic nanoparticles (MNPs) are important carriers in immunoassays. In this study, we investigated the influence of the physical and chemical properties of MNPs on their performance in a detection process. DESIGN AND METHODS A comparative study of the properties of two MNPs with sizes of 200nm and 1μm (MNP-200nm and MNP-1μm, respectively) was conducted using the following four aspects: nonspecific adsorption to proteins (IgG was used as a model protein), influence of magnetic nanoparticles on the chemiluminescence signal, response speed to an external magnetic field, and intensity of the detection signal. RESULTS MNP-1μm exhibited lower nonspecific adsorption to IgG in serum, a weaker interference with the chemiluminescence signal, and a higher response speed to the external magnetic field than the same amount of MNP-200nm. An automated chemiluminescence immunoassay system based on MNP-1μm was also established. CONCLUSIONS MNP-1μm acts as an excellent carrier in an automated chemiluminescence immunoassay system for the analysis of serum samples from clinical patients.

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