Evaluation of Ovaprim and Human Chorionic Gonadotropin Doses on Spawning Induction and Egg and Larval Quality of Pigfish, Orthopristis chrysoptera

Pigfish, Orthopristis chrysoptera, readily undergo vitellogenesis in captivity, but final oocyte maturation (FOM), ovulation, and spawning can be unpredictable. The objectives of this investigation were to assess the effects of various doses of Ovaprim® (sGnRHa [salmon gonadotropin-releasing hormone analog] + domperidone) and human chorionic gonadotropin (HCG) on FOM, ovulation, and spawning in pigfish. Ovaprim dosages investigated were 0.25, 0.50, 1.00, and 2.00 mL/kg, which were injected into the dorsal musculature of female pigfish. Male pigfish received one half the dosage administered to females to ensure spermiation. HCG doses of 500, 1000, 2000, and 4000 IU/kg were administered to female pigfish, with male pigfish once again receiving half the female dosage. Eggs were collected over a 72-h period postinjection and enumerated and assessed for fertilization, hatching percentage, and survival to first feeding. Eggs and larvae were photographed and a suite of morphological parameters was determined. Hormone dose evaluations with pigfish showed that the low-dose (0.25 and 0.50 mL/kg) Ovaprim treatments resulted in better spawning performance. However, superior egg and larval characteristics validate the 0.50 mL/kg dose as the preferred choice for use with pigfish. The four doses of HCG administered to pigfish performed unreliably in this study; thus, no dose recommendation could be made.

[1]  R. Lochmann,et al.  Hatchery methods and natural, hormone-implant-induced, and synchronized spawning of captive Atlantic croaker (Micropogonias undulatus) Linnaeus 1766 , 2010 .

[2]  M. DiMaggio,et al.  Induced Volitional Spawning and Larval Rearing of Pinfish , 2010 .

[3]  S. Giri,et al.  Effects of Latency Periods and Injection Doses with Carp Pituitary Extract on Spawning Performance and Egg Quality of Asian Catfish, Clarias batrachus (Linn.) , 2008 .

[4]  K. Riley,et al.  Volitional Spawning of Florida Pompano, Trachinotus carolinus, Induced via Administration of Gonadotropin Releasing Hormone Analogue (GnRHa) , 2007 .

[5]  S. Giri,et al.  Spawning performance and egg quality of Asian catfish Clarias batrachus (Linn.) at various doses of human chorionic gonadotropin (HCG) injection and latency periods during spawning induction , 2007 .

[6]  R. Phelps,et al.  Induced Spawning and Egg Quality Evaluation of Red Snapper, Lutjanus campechanus , 2007 .

[7]  Y. Zohar,et al.  Promoting oocyte maturation, ovulation and spawning in farmed fish , 2007 .

[8]  Theodore J. I. Smith,et al.  The use of luteinizing hormone releasing hormone analogue for ovulation induction in black sea bass (Centropristis striata) , 2005 .

[9]  S. K. Sahoo,et al.  Induced spawning of Asian catfish, Clarias batrachus (Linn.): effect of various latency periods and SGnRHa and domperidone doses on spawning performance and egg quality , 2005 .

[10]  Yifeng Chen Induced ovulation and embryonic development of ocellated puffer, Takifugu ocellatus , 2005 .

[11]  M. Oesterling,et al.  Production of spot as live bait for recreational angling , 2005 .

[12]  J. Rosas,et al.  Inducción hormonal al desove y descripción larval del corocoro Orthopristis ruber Cuvier (Pisces: Haemulidae) , 2004 .

[13]  Y. Zohar,et al.  Use of GnRHa-delivery systems for the control of reproduction in fish , 2000, Reviews in Fish Biology and Fisheries.

[14]  John P. Sumpter,et al.  Egg quality in fish: what makes a good egg? , 1997, Reviews in Fish Biology and Fisheries.

[15]  Y. Zohar Endocrinology and fish farming: Aspects in reproduction, growth, and smoltification , 1989, Fish Physiology and Biochemistry.

[16]  W. Watanabe,et al.  Volitional Spawning of Black Sea Bass Centropristis striata Induced with Pelleted Luteinizing Hormone Releasing Hormone‐Analogue , 2003 .

[17]  Y. Zohar,et al.  Endocrine manipulations of spawning in cultured fish: from hormones to genes , 2001 .

[18]  Masaru Tanaka,et al.  Early Life Stages in Japanese sea bass-II. Developmental Changes in Multiple Oil Globules of Japanese Sea Bass Eggs. , 1999 .

[19]  J. W. Tucker Marine Fish Culture , 1998 .

[20]  L. Colombo,et al.  Induction of spawning in gilthead seabream, Sparus aurata L., by a long-acting GnRH agonist and its effects on egg quality and daily timing of spawning , 1997 .

[21]  R. Patiño Manipulations of the Reproductive System of Fishes by Means of Exogenous Chemicals , 1997 .

[22]  M. Thrush,et al.  Broodstock management, fecundity, egg quality and the timing of egg production in the rainbow trout (Oncorhynchus mykiss) , 1992 .

[23]  E. M. Avila,et al.  Yolk and oil globule utilization and developmental morphology of the digestive tract epithelium in larval rabbitfish, Siganus guttatus (Bloch) , 1987 .

[24]  C. Schreck,et al.  Luteinizing hormone-releasing hormone analogue induces precocious ovulation in adult coho salmon (Oncorhynchus kisutch) , 1984 .

[25]  G. H. Darcy Synopsis of biological data on the pigfish, Orthopristis chrysoptera (pisces: haemulidae) , 1983 .

[26]  Y. Zohar,et al.  Spawning kinetics in the gilthead sea-bream, Sparus aurata L. after low doses of human chronic gonadotropin , 1979 .