ESTERASES IN DEVELOPING AND ADULT RAT LUNG II . AX ELECTROPHORETIC ANALYSIS ’

The esterases of developing rat lung have been investigated by means of disc gel dcctrophoresis of lung preparations. The enzymes were analyzed by a method that uses thiol esters as substrates in order to correlate these findings with earlier biochemical and cytochemical studies. The esters, p-nitrophenylthiol acetate and p-nitrophenylthiol butyrate, were hydrolyzed by the enzymes in the presence of copper ions. The resultant copper mercaptide was stained with dithiooxamide and analyzed visually and with a densitometer. Lung preparations from prenatal (days 18 through term), postnatal (through the 4th week) and adult animals were examined for the pattern of esterases and for their sensitivity to such inhibitors as 0, 0-diethyl O-p-nitrophenyl phosphate (E-600) and diisopropyl phosphorofluoridate (DFP) and to temperature inactivation. The zones of esterase activity increased in number and amount during development except for two bands which decreased markedly during late prenatal development. The timing of these changes correlates with various developmental events in the rat lung.

[1]  R. Schiff.  THE BIOCHEMICAL GENETICS OF RABBIT ERYTHROCYTE ESTERASES HISTOCHEMICAL CLASSIFICATION , 1970, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[2]  K. O'hare,et al.  Morphogenesis of albino rat lung: An autoradiographic analysis of the embryological origin of the type I and II pulmonary epithelial cells , 1970, Journal of morphology.

[3]  K. O'hare,et al.  Electron microscopic observations on the morphogenesis of the albino rat lung, with special reference to pulmonary epithelial cells. , 1970, The American journal of anatomy.

[4]  B. Corrin.  Phagocytic potential of pulmonary alveolar epithelium with particular reference to surfactant metabolism , 1970, Thorax.

[5]  A. Swan,et al.  Paraquat and related bipyridyls. , 1969, British medical bulletin.

[6]  C. Blackwood,et al.  Proteolytic enzyme systems in developing rat tissues. , 1968, Journal of reproduction and fertility.

[7]  K. McCarty,et al.  Esterase Activity in Embryonic Development∗ , 1968, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[8]  C. Masters,et al.  The ontogeny of pig and duck esterases. , 1968, Biochimica et biophysica acta.

[9]  P. Juul.  Human plasma cholinesterase isoenzymes. , 1968, Clinica chimica acta; international journal of clinical chemistry.

[10]  J. Lagnado,et al.  Brain Esterases during Development , 1967, Nature.

[11]  C. Masters,et al.  The developmental multiplicity and isoenzyme status of cavian esterases. , 1967, Biochimica et biophysica acta.

[12]  S. Sorokin.  A MORPHOLOGIC AND CYTOCHEMICAL STUDY ON THE GREAT ALVEOLAR CELL , 1966, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[13]  O. Reiss.  Studies of lung metabolism. I. Isolation and properties of subcellular fractions from rabbit lung. , 1966 .

[14]  Y. Suzuki.  The structural differentiation of the alveolar lining cells. I. Electron microscopic studies on the prospective alveolar epithelium in the lung tissue of rat embryos. , 1966, Okajimas folia anatomica Japonica.

[15]  Clark Dg,et al.  The Toxicity of Paraquat , 1966, British journal of industrial medicine.

[16]  R. Allen,et al.  SEPARATION AND RELATIVE QUANTITATION OF MOUSE PLASMA ESTERASES WITH DISC ELECTROPHORESIS , 1965, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[17]  S. Sommers,et al.  Pulmonary Alveolar Cell Inclusions: Their Development in the Rat , 1964, Science.

[18]  W. Kalow,et al.  PROPERTIES AND CLASSIFICATION OF THE SOLUBLE ESTERASES OF HUMAN KIDNEY. , 1964, Canadian journal of biochemistry.

[19]  R. Havel,et al.  Composition of surface-active material isolated from beef lung. , 1961, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Klas-Bertil Augustinsson,et al.  MULTIPLE FORMS OF ESTERASE IN VERTEBRATE BLOOD PLASMA * , 1961 .

[21]  R. E. Pattle,et al.  Lipoprotein Composition of the Film lining the Lung , 1961, Nature.

[22]  K. Augustinsson.  Electrophoretic Separation and Classification of Blood Plasma Esterases , 1958, Nature.

[23]  H. Eagle,et al.  Measurement of Cell Growth in Tissue Culture with a Phenol Reagent (Folin-Ciocalteau) , 1956, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[24]  C. Masters,et al.  The developmental multiplicity and isoenzyme status of rat esterases. , 1967, Biochimica et biophysica acta.

[25]  P. Conen,et al.  THE ROLE OF ALVEOLAR INCLUSION BODIES IN THE DEVELOPING LUNG. , 1964, Laboratory investigation; a journal of technical methods and pathology.

[26]  O. Svensmark.  ENZYMATIC AND MOLECULAR PROPERTIES OF CHOLINESTERASES IN HUMAN LIVER. , 1963, Acta physiologica Scandinavica.