A visual sensor array for pattern recognition analysis of proteins using novel blue-emitting fluorescent gold nanoclusters.

This paper describes a visual sensor array for pattern recognition analysis of proteins based on two different optical signal changes: colorimetric and fluorometric, by using two types of novel blue-emitting collagen protected gold nanoclusters and macerozyme R-10 protected gold nanoclusters with lower synthetic demands. Eight proteins have been well-discriminated by this visual sensor array, and protein mixtures after one-dimensional polyacrylamide gel electrophoresis also could be well-discriminated. The possible mechanism of this sensor array was illustrated and validated by fluorescence spectra, X-ray photoelectron spectroscopy (XPS), fluorescence lifetime, isothermal titration calorimetry (ITC), and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) experiments. It was attributed to that the adsorption of proteins onto the surface of gold nanoclusters (Au NCs), forming the protein-Au NCs complex. Furthermore, serums from normal and hepatoma patients were also effectively discriminated by this visual sensor array, showing feasible potential for diagnostic applications.

[1]  G. Malaguarnera,et al.  Serum Markers of Hepatocellular Carcinoma , 2010, Digestive Diseases and Sciences.

[2]  Jian Sun,et al.  Highly sensitive real-time assay of inorganic pyrophosphatase activity based on the fluorescent gold nanoclusters. , 2014, Analytical chemistry.

[3]  Huan‐Tsung Chang,et al.  One-pot synthesis of fluorescent BSA-Ce/Au nanoclusters as ratiometric pH probes. , 2014, Chemical communications.

[4]  Sichun Zhang,et al.  Lab-on-graphene: graphene oxide as a triple-channel sensing device for protein discrimination. , 2013, Chemical communications.

[5]  D C Carter,et al.  Structure of serum albumin. , 1994, Advances in protein chemistry.

[6]  Y. Taes,et al.  Direct CdTe quantum-dot-based fluorescence imaging of human serum proteins. , 2010, Small.

[7]  Jian-hui Jiang,et al.  Peptide-templated gold nanocluster beacon as a sensitive, label-free sensor for protein post-translational modification enzymes. , 2013, Analytical chemistry.

[8]  D C Carter,et al.  Three-dimensional structure of human serum albumin. , 1989, Science.

[9]  Yong Shao,et al.  DNA-hosted fluorescent gold nanoclusters: sequence-dependent formation , 2013, Nanotechnology.

[10]  P. Brick,et al.  Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites , 1998, Nature Structural Biology.

[11]  Dongil Lee,et al.  Ionic liquid of a gold nanocluster: a versatile matrix for electrochemical biosensors. , 2014, ACS nano.

[12]  Xin Lu,et al.  A highly sensitive “turn‐on” fluorescent sensor for the detection of human serum proteins based on the size exclusion of the polyacrylamide gel , 2014, Electrophoresis.

[13]  Yin-kun Liu,et al.  Diagnostic value of serum haptoglobin protein as hepatocellular carcinoma candidate marker complementary to α fetoprotein. , 2010, Oncology Report.

[14]  Yanhua Dong,et al.  Horseradish peroxidase functionalized fluorescent gold nanoclusters for hydrogen peroxide sensing. , 2011, Analytical chemistry.

[15]  Ding‐Shinn Chen,et al.  Identification of complement C3a as a candidate biomarker in human chronic hepatitis C and HCV‐related hepatocellular carcinoma using a proteomics approach , 2006, Proteomics.

[16]  H. Tian,et al.  Color-tunable solid-state emission of 2,2'-biindenyl-based fluorophores. , 2011, Angewandte Chemie.

[17]  Sichun Zhang,et al.  Protein discrimination using fluorescent gold nanoparticles on plasmonic substrates. , 2012, Analytical chemistry.

[18]  N. Khashab,et al.  "Light-on" sensing of antioxidants using gold nanoclusters. , 2014, Analytical chemistry.

[19]  Soft interactions at nanoparticles alter protein function and conformation in a size dependent manner. , 2011, Nano letters.

[20]  Robert C. Triulzi,et al.  Immunoasssay based on the antibody-conjugated PAMAM-dendrimer-gold quantum dot complex. , 2006, Chemical communications.

[21]  Chaoqing Dong,et al.  Sensitive single particle method for characterizing rapid rotational and translational diffusion and aspect ratio of anisotropic nanoparticles and its application in immunoassays. , 2013, Analytical chemistry.

[22]  C. Lau,et al.  "Turn-on" chemiluminescence sensor for the highly selective and ultrasensitive detection of Hg2+ ions based on interstrand cooperative coordination and catalytic formation of gold nanoparticles. , 2011, Analytical chemistry.

[23]  Pavel Bucek,et al.  A practical approach to optical cross-reactive sensor arrays. , 2010, Chemical Society reviews.

[24]  Robert L. Whetten,et al.  Visible to Infrared Luminescence from a 28-Atom Gold Cluster , 2002 .

[25]  P. Liao,et al.  Identification of human hepatocellular carcinoma-related proteins by proteomic approaches , 2007, Analytical and bioanalytical chemistry.

[26]  Duncan Graham,et al.  Quantitative detection of human tumor necrosis factor α by a resonance raman enzyme-linked immunosorbent assay. , 2011, Analytical chemistry.

[27]  Marc Schneider,et al.  Synthesis and characterization of human transferrin-stabilized gold nanoclusters , 2011, Nanotechnology.

[28]  P. Stadelmann,et al.  Crystallographic structure of small gold particles studied by high-resolution electron microscopy , 1991 .

[29]  C. Lin,et al.  Recombination dynamics of photoluminescence in thiol-protected gold nanoclusters , 2009 .

[30]  Y. Yue,et al.  Microwave-assisted synthesis of BSA-protected small gold nanoclusters and their fluorescence-enhanced sensing of silver(I) ions. , 2012, Nanoscale.

[31]  R. Murray,et al.  Reaction of Au55(PPh3)12Cl6 with thiols yields thiolate monolayer protected Au75 clusters , 2005 .

[32]  Juewen Liu,et al.  Blue emitting gold nanoclusters templated by poly-cytosine DNA at low pH and poly-adenine DNA at neutral pH. , 2012, Chemical communications.

[33]  G. Bazan,et al.  Conjugated Oligoelectrolyte/ssDNA Aggregates: Self‐Assembled Multicomponent Chromophores for Protein Discrimination , 2009 .

[34]  Yu-Chie Chen,et al.  Using protein-encapsulated gold nanoclusters as photoluminescent sensing probes for biomolecules. , 2014, Biosensors & bioelectronics.

[35]  Peter Claus,et al.  On the origin of binding energy shifts of core levels of supported gold nanoparticles and dependence of pretreatment and material synthesis , 2003 .

[36]  Sanjeeva Srivastava,et al.  Proteomic technologies for the identification of disease biomarkers in serum: Advances and challenges ahead , 2011, Proteomics.

[37]  Arabinda Mallick,et al.  Spectroscopic investigation on the interaction of ICT probe 3-acetyl-4-oxo-6,7-dihydro-12H Indolo-[2,3-a] quinolizine with serum albumins. , 2005, The journal of physical chemistry. B.

[38]  V. Rotello,et al.  Colorimetric protein sensing using catalytically amplified sensor arrays. , 2012, Small.

[39]  G. Nienhaus,et al.  Effect of protein adsorption on the fluorescence of ultrasmall gold nanoclusters. , 2012, Small.

[40]  P. Ross,et al.  Thermodynamics of protein association reactions: forces contributing to stability. , 1981, Biochemistry.

[41]  Yaolin Xu,et al.  The role of protein characteristics in the formation and fluorescence of Au nanoclusters. , 2014, Nanoscale.

[42]  Peng Zhang,et al.  Properties and applications of protein-stabilized fluorescent gold nanoclusters: short review , 2012 .

[43]  One-pot synthesis of blue light-emitting Au nanoclusters and formation of photo-patternable composite films. , 2011, Chemical communications.

[44]  Moon J. Kim,et al.  Luminescent Gold Nanoparticles with Mixed Valence States Generated from Dissociation of Polymeric Au (I) Thiolates. , 2010, The journal of physical chemistry. C, Nanomaterials and interfaces.

[45]  G. Nienhaus,et al.  Facile preparation of water-soluble fluorescent gold nanoclusters for cellular imaging applications. , 2011, Nanoscale.

[46]  W. Tseng,et al.  (Lysozyme type VI)-stabilized Au8 clusters: synthesis mechanism and application for sensing of glutathione in a single drop of blood. , 2012, Small.

[47]  R. Molina,et al.  Comparison of serum human epididymis protein 4 with cancer antigen 125 as a tumor marker in patients with malignant and nonmalignant diseases. , 2011, Clinical chemistry.

[48]  Y. Higashi,et al.  Regiospecific and Nonlinear Substituent Effects on the Electronic and Fluorescence Spectra of Phthalocyanines , 1995 .

[49]  Vincent M Rotello,et al.  Gold nanoparticle-fluorophore complexes: sensitive and discerning "noses" for biosystems sensing. , 2010, Angewandte Chemie.

[50]  R. Arakawa,et al.  ph‐Dependent Synthesis of Pepsin‐Mediated Gold Nanoclusters with Blue Green and Red Fluorescent Emission , 2011 .

[51]  G. Roberts,et al.  Detection of a protein conformational equilibrium by electrospray ionisation-ion mobility-mass spectrometry. , 2011, Angewandte Chemie.

[52]  Y. Hsiao,et al.  Insulin-directed synthesis of fluorescent gold nanoclusters: preservation of insulin bioactivity and versatility in cell imaging. , 2011, Angewandte Chemie.