Development of a micronucleus test using the EpiAirway™ organotypic human airway model

[1]  R. Bedford,et al.  Recent advancements and application of in vitro models for predicting inhalation toxicity in humans. , 2021, Toxicology in vitro : an international journal published in association with BIBRA.

[2]  C. Li,et al.  Rapid and automatic detection of micronuclei in binucleated lymphocytes image , 2021, Scientific Reports.

[3]  C. Ehrhardt,et al.  In vitro and ex vivo models in inhalation biopharmaceutical research - advances, challenges and future perspectives. , 2021, Advanced drug delivery reviews.

[4]  Elizabeth K. Schmidt,et al.  Genetic toxicity testing using human in vitro organotypic airway cultures: Assessing DNA damage with the CometChip and mutagenesis by Duplex Sequencing , 2021, Environmental and molecular mutagenesis.

[5]  Anne E Carpenter,et al.  Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learning , 2021, Archives of Toxicology.

[6]  S. Pfuhler,et al.  The 3D reconstructed skin micronucleus assay using imaging flow cytometry and deep learning: A proof-of-principle investigation. , 2021, Mutation research.

[7]  G. Jenkins,et al.  Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure. , 2020, Journal of visualized experiments : JoVE.

[8]  Andrew Williams,et al.  Flow cytometric micronucleus assay and TGx-DDI transcriptomic biomarker analysis of ten genotoxic and non-genotoxic chemicals in human HepaRG™ cells , 2020, Genes and Environment.

[9]  T. Sofuni,et al.  Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT). , 2020, Mutation research.

[10]  Lei Guo,et al.  Effects of cellular differentiation in human primary bronchial epithelial cells: Metabolism of 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone. , 2019, Toxicology in vitro : an international journal published in association with BIBRA.

[11]  C. Bolognesi,et al.  Cytome micronucleus assays with a metabolically competent human derived liver cell line (Huh6): A promising approach for routine testing of chemicals? , 2018, Environmental and molecular mutagenesis.

[12]  H. Rosenfeldt,et al.  Evaluating Mode of Action of Acrolein Toxicity in an In Vitro Human Airway Tissue Model , 2018, Toxicological sciences : an official journal of the Society of Toxicology.

[13]  Paul Walker,et al.  Innovative organotypic in vitro models for safety assessment: aligning with regulatory requirements and understanding models of the heart, skin, and liver as paradigms , 2018, Archives of Toxicology.

[14]  Raffaella Corvi,et al.  In vitro genotoxicity testing-Can the performance be enhanced? , 2017, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[15]  Ashraf Elamin,et al.  A systems toxicology approach for comparative assessment: Biological impact of an aerosol from a candidate modified-risk tobacco product and cigarette smoke on human organotypic bronchial epithelial cultures. , 2017, Toxicology in vitro : an international journal published in association with BIBRA.

[16]  Hao Zhu,et al.  Analysis of Draize Eye Irritation Testing and its Prediction by Mining Publicly Available 2008–2014 REACH Data , 2016, ALTEX.

[17]  E. Minet,et al.  Targeted omics analyses, and metabolic enzyme activity assays demonstrate maintenance of key mucociliary characteristics in long term cultures of reconstituted human airway epithelia. , 2015, Toxicology in vitro : an international journal published in association with BIBRA.

[18]  L. Muskhelishvili,et al.  Tight junction disruption by cadmium in an in vitro human airway tissue model , 2015, Respiratory Research.

[19]  S. Hoffmann,et al.  Retrospective analysis of the Draize test for serious eye damage/eye irritation: importance of understanding the in vivo endpoints under UN GHS/EU CLP for the development and evaluation of in vitro test methods , 2013, Archives of Toxicology.

[20]  André Guillouzo,et al.  An adaptation of the human HepaRG cells to the in vitro micronucleus assay. , 2012, Mutagenesis.

[21]  Edward S. Kim,et al.  Prevention of Bronchial Hyperplasia by EGFR Pathway Inhibitors in an Organotypic Culture Model , 2011, Cancer Prevention Research.

[22]  A. Guillouzo,et al.  Assessment of the genotoxic potential of indirect chemical mutagens in HepaRG cells by the comet and the cytokinesis-block micronucleus assays. , 2010, Mutagenesis.

[23]  Nicola J Hewitt,et al.  International prevalidation studies of the EpiDerm 3D human reconstructed skin micronucleus (RSMN) assay: transferability and reproducibility. , 2010, Mutation research.

[24]  M. de Jong,et al.  Of Mice and Humans: Are They the Same?—Implications in Cancer Translational Research , 2010, Journal of Nuclear Medicine.

[25]  G. Jackson,et al.  Drug metabolizing enzyme activity of in vitro human dermal (EpiDerm™) and airway (EpiAirway™) epithelial models: Relationship to genotoxicity of chemicals as determined by in vitro skin micronucleus assays , 2009 .

[26]  S Albertini,et al.  Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test. I. Theoretical aspects. , 2008, Mutation research.

[27]  Raffaella Corvi,et al.  ECVAM retrospective validation of in vitro micronucleus test (MNT) , 2008, Mutagenesis.

[28]  R. Lotan,et al.  Proteomics-based identification of proteins secreted in apical surface fluid of squamous metaplastic human tracheobronchial epithelial cells cultured by three-dimensional organotypic air-liquid interface method. , 2007, Cancer research.

[29]  Albrecht Poth,et al.  Genetic toxicity assessment: employing the best science for human safety evaluation part IV: Recommendation of a working group of the Gesellschaft fuer Umwelt-Mutationsforschung (GUM) for a simple and straightforward approach to genotoxicity testing. , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[30]  G. Jackson,et al.  Drug/xenobiotic-metabolizing enzyme (XME) expression in the EpiAirway in vitro human airway model: Utility for assessing tracheal/bronchial biotransformation of inhaled pharmaceuticals and environmental chemicals , 2006 .

[31]  Marilyn J Aardema,et al.  Development of a method for assessing micronucleus induction in a 3D human skin model (EpiDerm). , 2006, Mutation research.

[32]  J. Koo,et al.  Nonclassical action of retinoic acid on the activation of the cAMP response element-binding protein in normal human bronchial epithelial cells. , 2005, Molecular biology of the cell.

[33]  A. Pedram,et al.  Cytochalasin B modulation of Caco-2 tight junction barrier: role of myosin light chain kinase. , 2000, American journal of physiology. Gastrointestinal and liver physiology.

[34]  T. Sofuni,et al.  Validation study of the in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU). , 1999, Mutagenesis.

[35]  J. Koo,et al.  Role of retinoid receptors in the regulation of mucin gene expression by retinoic acid in human tracheobronchial epithelial cells. , 1999, The Biochemical journal.

[36]  V. Mersch-Sundermann,et al.  Genotoxic effects of three Fusarium mycotoxins, fumonisin B1, moniliformin and vomitoxin in bacteria and in primary cultures of rat hepatocytes. , 1997, Mutation research.

[37]  A. Zijno,et al.  Simultaneous detection of X-chromosome loss and non-disjunction in cytokinesis-blocked human lymphocytes by in situ hybridization with a centromeric DNA probe; implications for the human lymphocyte in vitro micronucleus assay using cytochalasin B. , 1994, Mutagenesis.

[38]  Y. Rojanasakul,et al.  The cytoskeleton of the cornea and its role in tight junction permeability , 1991 .

[39]  S. Carter Effects of Cytochalasins on Mammalian Cells , 1967, Nature.

[40]  J. Haycock,et al.  State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology. , 2014, ALTEX.

[41]  Hilde van der Togt,et al.  Publisher's Note , 2003, J. Netw. Comput. Appl..

[42]  D. Lloyd,et al.  Radiation induction of micronuclei in human lymphocytes. , 1988, Mutation research.