Structural studies of MJ1529, an O6‐methylguanine‐DNA methyltransferase

The structure of an O6‐methylguanine‐DNA methyltransferase (MGMT) from the thermophile Methanococcus jannaschii has been determined using multinuclear multidimensional NMR spectroscopy. The structure is similar to homologs from other organisms that have been determined by crystallography, with some variation in the N‐terminal domain. The C‐terminal domain is more highly conserved in both sequence and structure. Regions of the protein show broadening, reflecting conformational flexibility that is likely related to function. Copyright © 2006 John Wiley & Sons, Ltd.

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