Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water

Irgarol 1051 is one of the most employed antifouling agents and is one of the most commonly found in marine environment. According to its widespread use the European Comission turned its attention on this agent as one of the priority substances that has to be controlled. The aim of this article is to develop an immunosensor for the determination of Irgarol 1051 in sea water for implementation in a flow analysis system. The transducing principle employed for its development was an amperometric detection using gold screen printed electrodes which were functionalized for the covalent immobilization of the immunoreagents. The detection of Irgarol 1051 was performed using specific antibodies working under competitive indirect format. The immunosensor was demonstrated to measure directly in sea water with high detectability, accurately and robust. The system allows consecutive measurements using the same chip owing to an easy regeneration process. The limit of detection reached was 0.15±0.09 nM (0.038±0.022 µg ⋅ L−1) measured directly in sea water.

[1]  H. Budzinski,et al.  Pharmaceuticals, alkylphenols and pesticides in Mediterranean coastal waters: Results from a pilot survey using passive samplers , 2012 .

[2]  N. Sanvicens,et al.  Development of an immunoassay for terbutryn: study of the influence of the immunization protocol. , 2012, Talanta.

[3]  R. Puchades,et al.  Optical immunosensors for environmental monitoring: How far have we come? , 2006, Analytical and bioanalytical chemistry.

[4]  Akito Tanaka,et al.  Reduction of nonspecific binding proteins to self-assembled monolayer on gold surface. , 2006, Bioorganic & medicinal chemistry.

[5]  F. Borrull,et al.  Monitoring of antifouling agents in water samples by on-line solid-phase extraction-liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. , 2001, Journal of chromatography. A.

[6]  J. Readman,et al.  Antifouling paint booster biocides in the UK coastal environment and potential risks of biological effects. , 2001, Marine pollution bulletin.

[7]  Gajendra S Shekhawat,et al.  Immunoanalytical techniques for analyzing pesticides in the environment , 2009 .

[8]  A. Fernández-Alba,et al.  Determination of traces of five antifouling agents in water by gas chromatography with positive/negative chemical ionisation and tandem mass spectrometric detection. , 2001, Journal of chromatography. A.

[9]  N. Verma,et al.  Biosensor Technology for Pesticides—A review , 2015, Applied Biochemistry and Biotechnology.

[10]  Yibin Ying,et al.  Immunosensors for detection of pesticide residues. , 2008, Biosensors & bioelectronics.

[11]  Ángel Maquieira,et al.  An Immunosensor for the Automatic Determination of the Antifouling Agent Irgarol 1051 in Natural Waters , 1998 .

[12]  M Pilar Marco,et al.  Portable surface plasmon resonance immunosensor for the detection of fluoroquinolone antibiotic residues in milk. , 2011, Journal of agricultural and food chemistry.

[13]  Judith Rishpon,et al.  Electrochemical Biosensors for Pollutants in the Environment , 2007 .

[14]  D. Barceló,et al.  Preparation of antisera and development of a direct enzyme-linked immunosorbent assay for the determination of the antifouling agent Irgarol 1051 , 1997 .

[15]  D Barceló,et al.  Part-per-trillion level determination of antifouling pesticides and their byproducts in seawater samples by off-line solid-phase extraction followed by high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. , 2000, Journal of chromatography. A.

[16]  C. P. Swart,et al.  Determination of diuron and the antifouling paint biocide irgarol 1051 in Dutch marinas and coastal waters. , 2002, Journal of chromatography. A.

[17]  Z. Sosa-Ferrera,et al.  Probabilistic risk assessment of common booster biocides in surface waters of the harbours of Gran Canaria (Spain). , 2011, Marine pollution bulletin.

[18]  D Barceló,et al.  Influence of the hapten design on the development of a competitive ELISA for the determination of the antifouling agent Irgarol 1051 at trace levels. , 1998, Analytical chemistry.

[19]  Peter Dubruel,et al.  Recent advances in recognition elements of food and environmental biosensors: a review. , 2010, Biosensors & bioelectronics.

[20]  M. M. Arifin,et al.  Occurrence and distribution of antifouling biocide Irgarol-1051 in coastal waters of Peninsular Malaysia. , 2013, Marine pollution bulletin.

[21]  J. Villeneuve,et al.  Coastal water contamination from a triazine herbicide used in antifouling paints , 1993 .

[22]  B. Harris,et al.  Exploiting antibody-based technologies to manage environmental pollution. , 1999, Trends in biotechnology.

[23]  S. Dong,et al.  Electrochemical biosensing in extreme environment , 2002 .

[24]  Heinrich Hühnerfuss,et al.  Concentrations of the Antifouling Compound Irgarol 1051 and of Organotins in Water and Sediments of German North and Baltic Sea Marinas , 2000 .

[25]  T. Albanis,et al.  Worldwide occurrence and effects of antifouling paint booster biocides in the aquatic environment: a review. , 2004, Environment international.

[26]  Marek Piliarik,et al.  A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples. , 2010, Biosensors & bioelectronics.

[27]  S. Molaei,et al.  Microfunnel-supported liquid-phase microextraction: application to extraction and determination of Irgarol 1051 and diuron in the Persian Gulf seawater samples. , 2014, Journal of chromatography. A.

[28]  D. Barceló,et al.  Immunosensor for trace determination of Irgarol 1051 in seawater using organic media , 1999 .

[29]  Damià Barceló,et al.  Achievements of the RIANA and AWACSS EU Projects: Immunosensors for the Determination of Pesticides, Endocrine Disrupting Chemicals and Pharmaceuticals , 2009 .

[30]  Ronald D. Anderson,et al.  Monitoring of Irgarol 1051 concentrations with concurrent phytoplankton evaluations in East Coast areas of the United States. , 2005, Marine Pollution Bulletin.

[31]  T. Albanis,et al.  Comparison of the performance of analytical methods based on solid-phase extraction and on solid-phase microextraction for the determination of antifouling booster biocides in natural waters , 2002 .