Microbiological Sensing Technologies: A Review

Microorganisms have a significant influence on human activities and health, and consequently, there is high demand to develop automated, sensitive, and rapid methods for their detection. These methods might be applicable for clinical, industrial, and environmental applications. Although different techniques have been suggested and employed for the detection of microorganisms, and the majority of these methods are not cost effective and suffer from low sensitivity and low specificity, especially in mixed samples. This paper presents a comprehensive review of microbiological techniques and associated challenges for bioengineering researchers with an engineering background. Also, this paper reports on recent technological advances and their future prospects for a variety of microbiological applications.

[1]  A. Muro,et al.  Development of a Highly Sensitive Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Loa loa , 2014, PloS one.

[2]  Y. Sugita,et al.  TiO2 micro-nano-hybrid surface to alleviate biological aging of UV-photofunctionalized titanium , 2011, International journal of nanomedicine.

[3]  Claudio Parolo,et al.  Paper-based electroanalytical devices with an integrated, stable reference electrode. , 2013, Lab on a chip.

[4]  H. Andersson,et al.  Microfluidic devices for cellomics: a review , 2003 .

[5]  L. Rossetti,et al.  Rapid identification of dairy lactic acid bacteria by M13-generated, RAPD-PCR fingerprint databases. , 2005, Journal of microbiological methods.

[6]  Ernesto Picardi,et al.  Good laboratory practices and L.I.M.S. system: the challenge for a Next Generation Sequencing and bioinformatic research laboratory , 2017 .

[7]  M. Belleville,et al.  Biochips on CMOS: an active matrix address array for DNA analysis , 1999 .

[8]  Firouz Abbasian,et al.  Metagenomic Study of Human Gastrointestinal Tracts in Health and Diseases , 2013 .

[9]  Stefan Kopetz,et al.  PDMS-based optical waveguide layer for integration in electrical–optical circuit boards , 2007 .

[10]  C. Hannoun,et al.  Comparison between terminal-restriction fragment length polymorphism (T-RFLP) and quantitative culture for analysis of infants' gut microbiota. , 2013, Journal of microbiological methods.

[11]  Stephen A. Boppart,et al.  Use of molecular beacons for the detection of bacteria in microfluidic devices , 2003, SPIE MOEMS-MEMS.

[12]  Nicole Jaffrezic-Renault,et al.  Label-free detection of bacteria by electrochemical impedance spectroscopy: comparison to surface plasmon resonance. , 2007, Analytical chemistry.

[13]  Donald C. Lehman,et al.  Textbook of Diagnostic Microbiology , 1997 .

[14]  C. Hill,et al.  Silane coupling agents used for natural fiber/polymer composites: A review , 2010 .

[15]  L. Brocchieri.  The GC Content of Bacterial Genomes , 2014 .

[16]  H. Frickmann,et al.  Fluorescence in situ hybridization (FISH) in the microbiological diagnostic routine laboratory: a review , 2017, Critical reviews in microbiology.

[17]  C. Thermes,et al.  Ten years of next-generation sequencing technology. , 2014, Trends in genetics : TIG.

[18]  Peter J. Coassin,et al.  Cyclic olefin polymers: innovative materials for high-density multiwell plates. , 2008, Assay and drug development technologies.

[19]  S. Piletsky,et al.  Surface Functionalization of Porous Polypropylene Membranes with Molecularly Imprinted Polymers by Photograft Copolymerization in Water , 2000 .

[20]  N. Yusof,et al.  The strategies of DNA immobilization and hybridization detection mechanism in the construction of electrochemical DNA sensor: A review , 2017 .

[21]  D. Žgur-Bertok,et al.  Escherichia coli Bacteriocins: Antimicrobial Efficacy and Prevalence among Isolates from Patients with Bacteraemia , 2011, PloS one.

[22]  Zhouping Wang,et al.  Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labels. , 2014, Analytical chemistry.

[23]  Array of biosensors for discrimination of grapes according to grape variety, vintage and ripeness. , 2016, Analytica chimica acta.

[24]  Efstratios Skafidas,et al.  Lab on a chip sensor for rapid detection and antibiotic resistance determination of Staphylococcus aureus. , 2016, The Analyst.

[25]  M. Heller.  DNA microarray technology: devices, systems, and applications. , 2002, Annual review of biomedical engineering.

[26]  J. Riu,et al.  Graphene-based potentiometric biosensor for the immediate detection of living bacteria. , 2014, Biosensors & bioelectronics.

[27]  K.,et al.  Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A , 1975, Journal of bacteriology.

[28]  T. Livache,et al.  Biotinylated polypyrrole films: an easy electrochemical approach for the reagentless immobilization of bacteria on electrode surfaces. , 2004, Bioelectrochemistry.

[29]  Hermann E. Gaub,et al.  From Genes to Protein Mechanics on a Chip , 2014, Nature Methods.

[30]  Joseph Wang,et al.  Electrochemical sensors, biosensors, and their biomedical applications , 2008 .

[31]  E Baldrich,et al.  Impedance biosensing using phages for bacteria detection: generation of dual signals as the clue for in-chip assay confirmation. , 2010, Biosensors & bioelectronics.

[32]  Chih-Ming Ho,et al.  Surface molecular property modifications for poly(dimethylsiloxane) (PDMS) based microfluidic devices , 2009, Microfluidics and nanofluidics.

[33]  J. Baudart,et al.  Aptasensor and genosensor methods for detection of microbes in real world samples. , 2013, Methods.

[34]  M. Devare,et al.  Fatty acid profiles of PUFA producing Antarctic bacteria: correlation with RAPD analysis , 2010, Annals of Microbiology.

[35]  Yi-Wei Tang,et al.  Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology , 2009, Clinical Microbiology Reviews.

[36]  K. Müllen,et al.  Surface Functionalized ZnO Particles Designed for the Use in Transparent Nanocomposites , 2005 .

[37]  Chen-zhong Li,et al.  Amperometric micro-immunosensor for the detection of tumor biomarker. , 2009, Biosensors & bioelectronics.

[38]  Jinyang Chung,et al.  Aptamer cocktails: enhancement of sensing signals compared to single use of aptamers for detection of bacteria. , 2014, Biosensors & bioelectronics.

[39]  B. MacCraith,et al.  Immobilization of biomolecules on cycloolefin polymer supports. , 2007, Analytical chemistry.

[40]  J. Neilands,et al.  Siderophore protection against colicins M, B, V, and Ia in Escherichia coli , 1976, Journal of bacteriology.

[41]  S. Guterman.  Colicin B: Mode of Action and Inhibition by Enterochelin , 1973, Journal of bacteriology.

[42]  I. Park,et al.  Thiolated Salmonella antibody immobilization onto the gold surface of piezoelectric quartz crystal. , 1998, Biosensors & bioelectronics.

[43]  Yan Lian,et al.  A new aptamer/graphene interdigitated gold electrode piezoelectric sensor for rapid and specific detection of Staphylococcus aureus. , 2015, Biosensors & bioelectronics.

[44]  L. Rieseberg,et al.  A genomic perspective on hybridization and speciation , 2016, Molecular ecology.

[45]  Yasuyoshi Mori,et al.  Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects , 2015, Journal of Microbiology.

[46]  Chunsheng Wu,et al.  Cell-based biosensors and their application in biomedicine. , 2014, Chemical reviews.

[47]  A. Ramamoorthy,et al.  The spectrum of antimicrobial activity of the bacteriocin subtilosin A. , 2006, The Journal of antimicrobial chemotherapy.

[48]  J. G. E. Gardeniers,et al.  Lab-On-A-Chip Systems For Biomedical And Environmental Monitoring , 2003, Int. J. Comput. Eng. Sci..

[49]  M. Tabrizian,et al.  Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics. , 2012, Lab on a chip.

[50]  Rashid Bashir,et al.  Electrical/electrochemical impedance for rapid detection of foodborne pathogenic bacteria. , 2008, Biotechnology advances.

[51]  S. Pack,et al.  Direct immobilization of DNA oligomers onto the amine-functionalized glass surface for DNA microarray fabrication through the activation-free reaction of oxanine , 2007, Nucleic acids research.

[52]  Jim F Huggett,et al.  Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus. , 2014, Analytical chemistry.

[53]  R. Zengerle,et al.  Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. , 2010, Chemical Society reviews.

[54]  Po-Chiao Lin,et al.  Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles , 2013, Journal of analytical methods in chemistry.

[55]  Michihiko Nakano,et al.  A rapid bacteria detection technique utilizing impedance measurement combined with positive and negative dielectrophoresis , 2013 .

[56]  N. Jiao,et al.  Developing a Novel Approach of rpoB Gene as a Powerful Biomarker for the Environmental Microbial Diversity , 2013 .

[57]  An integrated microfluidic chip for the detection of bacteria - A proof of concept. , 2015, Molecular and cellular probes.

[58]  J. Feijen,et al.  Protein immobilization strategies for protein biochips. , 2007, Biomacromolecules.

[59]  Luke P. Lee,et al.  Innovations in optical microfluidic technologies for point-of-care diagnostics. , 2008, Lab on a chip.

[60]  Aleksandr Simonian,et al.  Microbial biosensors based on potentiometric detection , 1998 .

[61]  W Hampton Henley,et al.  A microfluidic chip integrating DNA extraction and real-time PCR for the detection of bacteria in saliva. , 2013, Lab on a chip.

[62]  Noemí de-los-Santos-Álvarez,et al.  A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins , 2013 .

[63]  Abolfazl Akbarzadeh,et al.  Application of gold nanoparticles in biomedical and drug delivery , 2016, Artificial cells, nanomedicine, and biotechnology.

[64]  D. Ginzinger.  Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. , 2002, Experimental hematology.

[65]  R. Naidu,et al.  The integration of sequencing and bioinformatics in metagenomics , 2015, Reviews in Environmental Science and Bio/Technology.

[66]  M. Aizawa,et al.  Electrochemical typing of blood using affinity membranes , 1980 .

[67]  Zhi-ping Zhang,et al.  DNA probe functionalized QCM biosensor based on gold nanoparticle amplification for Bacillus anthracis detection. , 2011, Biosensors & bioelectronics.

[68]  M. Chiao,et al.  Anti-fouling Coatings of Poly(dimethylsiloxane) Devices for Biological and Biomedical Applications , 2015, Journal of Medical and Biological Engineering.

[69]  R. Deshmukh,et al.  Diversity Analysis of Bacillus thuringiensis Isolates Recovered from Diverse Habitats in India using Random Amplified Polymorphic DNA (RAPD) Markers , 2013 .

[70]  S. Campuzano,et al.  Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates. , 2016, Analytica chimica acta.

[71]  D. Botstein,et al.  Construction of a genetic linkage map in man using restriction fragment length polymorphisms. , 1980, American journal of human genetics.

[72]  F. Baker,et al.  Introduction to Medical Laboratory Technology , 1976 .

[73]  J. E. Turner,et al.  BacT/Alert: an automated colorimetric microbial detection system , 1990, Journal of clinical microbiology.

[74]  D. Mock,et al.  Release of biotin from biotinylated proteins occurs enzymatically and nonenzymatically in human plasma. , 2004, Analytical biochemistry.

[75]  Yixian Wang,et al.  Application of Aptamer Based Biosensors for Detection of Pathogenic Microorganisms , 2012, Chinese Journal of Analytical Chemistry.

[76]  P. Skládal,et al.  Electrochemical biosensors - principles and applications , 2008 .

[77]  Niina J. Ronkainen,et al.  Electrochemical biosensors. , 2010, Chemical Society reviews.

[78]  Raymond J Carroll,et al.  DNA Microarray Experiments: Biological and Technological Aspects , 2002, Biometrics.

[79]  S. Niemann,et al.  Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis , 2015, Journal of Clinical Microbiology.

[80]  J. Vörös,et al.  Electrochemical Biosensors - Sensor Principles and Architectures , 2008, Sensors.

[81]  Haibo Zhou,et al.  Label and label-free based surface-enhanced Raman scattering for pathogen bacteria detection: A review. , 2017, Biosensors & bioelectronics.

[82]  Qingjun Liu,et al.  Biosensors and bioelectronics on smartphone for portable biochemical detection. , 2016, Biosensors & bioelectronics.

[83]  Jo V. Rushworth,et al.  Biosensors for Whole-Cell Bacterial Detection , 2014, Clinical Microbiology Reviews.

[84]  Tuan Vo-Dinh,et al.  A compact CMOS biochip immunosensor towards the detection of a single bacteria. , 2005, Biosensors & bioelectronics.

[85]  Darwin R. Reyes,et al.  Micro total analysis systems. 1. Introduction, theory, and technology. , 2002, Analytical chemistry.

[86]  Philip Brisk,et al.  Recent developments in microfluidic large scale integration. , 2014, Current opinion in biotechnology.

[87]  Shu-jen Han,et al.  CMOS Integrated DNA Microarray Based on GMR Sensors , 2006, 2006 International Electron Devices Meeting.

[88]  Junya Suehiro,et al.  Improvement of electric pulse shape for electropermeabilization-assisted dielectrophoretic impedance measurement for high sensitive bacteria detection , 2005 .

[89]  Sohini Ramachandran,et al.  Global rise in human infectious disease outbreaks , 2014, Journal of The Royal Society Interface.

[90]  Shuyu Xie,et al.  Qualitative screening of veterinary anti-microbial agents in tissues, milk, and eggs of food-producing animals using liquid chromatography coupled with tandem mass spectrometry. , 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[91]  A. Herr,et al.  Protein immobilization techniques for microfluidic assays. , 2013, Biomicrofluidics.

[92]  Alison M. Cupples,et al.  Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9 , 2016, Applied and Environmental Microbiology.

[93]  Liju Yang,et al.  Electrical impedance spectroscopy for detection of bacterial cells in suspensions using interdigitated microelectrodes. , 2008, Talanta.

[94]  C. Tebbe,et al.  Profiling the diversity of microbial communities with single-strand conformation polymorphism (SSCP). , 2014, Methods in molecular biology.

[95]  P. Glenn Gulak,et al.  Rapid Bacterial Detection via an All-Electronic CMOS Biosensor , 2016, PloS one.

[96]  C. Manaia,et al.  Applications of optical DNA mapping in microbiology. , 2017, BioTechniques.

[97]  F. Hauquier,et al.  Boronic Acid-Functionalized Oxide-Free Silicon Surfaces for the Electrochemical Sensing of Dopamine. , 2017, Langmuir : the ACS journal of surfaces and colloids.

[98]  Xinqiao Jia,et al.  Controlling the adhesion and differentiation of mesenchymal stem cells using hyaluronic acid-based, doubly crosslinked networks. , 2011, Biomaterials.

[99]  S. Weisberg,et al.  A review of technologies for rapid detection of bacteria in recreational waters. , 2005, Journal of water and health.

[100]  Kin Fong Lei,et al.  Review on Impedance Detection of Cellular Responses in Micro/Nano Environment , 2014, Micromachines.

[101]  Mick Watson,et al.  Successful test launch for nanopore sequencing , 2015, Nature Methods.

[102]  Il-Hoon Cho,et al.  Ultrasensitive detection of microbial cells using magnetic focus enhanced lateral flow sensors. , 2016, Chemical communications.

[103]  Chit Yaw Fu,et al.  Integration of optical fiber light guide, fluorescence detection system, and multichannel disposable microfluidic chip , 2007, Biomedical microdevices.

[104]  Zhijuan Cao,et al.  G-rich sequence-functionalized polystyrene microsphere-based instantaneous derivatization for the chemiluminescent amplified detection of DNA. , 2010, Luminescence : the journal of biological and chemical luminescence.

[105]  O. Inatomi,et al.  Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn’s disease using terminal restriction fragment length polymorphism analysis , 2011, Journal of Gastroenterology.

[106]  J. T. Dunnen,et al.  Next generation sequencing technology: Advances and applications. , 2014, Biochimica et biophysica acta.

[107]  A. Mirzabekov,et al.  Portable System for Microbial Sample Preparation and Oligonucleotide Microarray Analysis , 2001, Applied and Environmental Microbiology.

[108]  Shenguang Ge,et al.  Paper-based chemiluminescence ELISA: lab-on-paper based on chitosan modified paper device and wax-screen-printing. , 2012, Biosensors & bioelectronics.

[109]  P. Telleman,et al.  Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays. , 2004, Nucleic acids research.

[110]  R. Pethig,et al.  Applications of dielectrophoresis in biotechnology. , 1997, Trends in biotechnology.

[111]  Tae-Hyeong Kim,et al.  Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens. , 2014, Analytical chemistry.

[112]  Francisco M. Cornejo-Castillo,et al.  Metagenomic 16S rDNA Illumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities. , 2014, Environmental microbiology.

[113]  Se-Hwan Paek,et al.  CMOS image sensor-based ELISA detector using lens-free shadow imaging platform , 2014 .

[114]  R. Sharma,et al.  Single-Strand Conformational Polymorphism of EST-SSRs: A Potential Tool for Diversity Analysis and Varietal Identification in Sugarcane , 2011, Plant Molecular Biology Reporter.

[115]  S. Sze.  Semiconductor Devices: Physics and Technology , 1985 .

[116]  Thierry Livache,et al.  On-chip microbial culture for the specific detection of very low levels of bacteria. , 2013, Lab on a chip.

[117]  S. Kashid,et al.  Antibody tagged gold nanoparticles as scattering probes for the pico molar detection of the proteins in blood serum using nanoparticle tracking analyzer. , 2015, Colloids and surfaces. B, Biointerfaces.

[118]  Jie Xu,et al.  Application of microfluidic “lab-on-a-chip” for the detection of mycotoxins in foods , 2015 .

[119]  L. Blum,et al.  DNA biosensors and microarrays. , 2008, Chemical reviews.

[120]  M. S. Piedade,et al.  Integration of Magnetoresistive Biochips on a CMOS Circuit , 2012, IEEE Transactions on Magnetics.

[121]  E. Ashley,et al.  Stable, Covalent Attachment of Laminin to Microposts Improves the Contractility of Mouse Neonatal Cardiomyocytes , 2014, ACS applied materials & interfaces.

[122]  Yi Wang,et al.  A 3D-impedimetric immunosensor based on foam Ni for detection of sulfate-reducing bacteria , 2010 .

[123]  V. Wu,et al.  A piezoelectric immunosensor for specific capture and enrichment of viable pathogens by quartz crystal microbalance sensor, followed by detection with antibody-functionalized gold nanoparticles. , 2012, Biosensors & bioelectronics.

[124]  Edoardo Charbon,et al.  Towards large scale CMOS single-photon detector arrays for lab-on-chip applications , 2008 .

[125]  Gabriela Kuncová,et al.  Fiber-Optic Chemical Sensors and Fiber-Optic Bio-Sensors , 2015, Sensors.

[126]  D. Kuritzkes,et al.  Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens. , 2017, Nanoscale.

[127]  Changjun Hou,et al.  Microbial biosensors: a review. , 2011, Biosensors & bioelectronics.

[128]  Jing Jiang,et al.  Cellphone based Portable Bacteria Pre-Concentrating microfluidic Sensor and Impedance Sensing System , 2013, 1312.0329.

[129]  David T. F. Dryden,et al.  Type I restriction enzymes and their relatives , 2013, Nucleic acids research.

[130]  S. Soda,et al.  Characterization of microorganisms at different landfill depths using carbon-utilization patterns and 16S rRNA gene based T-RFLP. , 2010, Journal of bioscience and bioengineering.

[131]  D. Mooney,et al.  Hydrogels for tissue engineering: scaffold design variables and applications. , 2003, Biomaterials.

[132]  M. Wang,et al.  Diversity analysis of bacterial community compositions in sediments of urban lakes by terminal restriction fragment length polymorphism (T-RFLP) , 2012, World Journal of Microbiology and Biotechnology.

[133]  Teodor Veres,et al.  Integration and detection of biochemical assays in digital microfluidic LOC devices. , 2010, Lab on a chip.

[134]  R. Lakshmi,et al.  Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix. , 1999, Analytical biochemistry.

[135]  J. Guillamón,et al.  A new simplified AFLP method for wine yeast strain typing , 2010 .

[136]  M. M. Rueda,et al.  A PCR-RFLP method for the simultaneous differentiation of three Entamoeba species. , 2015, Experimental parasitology.

[137]  Y. Gho,et al.  Proteomics, transcriptomics and lipidomics of exosomes and ectosomes , 2013, Proteomics.

[138]  K. Neoh,et al.  Antibacterial and antifungal efficacy of surface functionalized polymeric beads in repeated applications. , 2005, Biotechnology and bioengineering.

[139]  J. Sattabongkot,et al.  Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Malaria Infections in an Area of Endemicity in Thailand , 2014, Journal of Clinical Microbiology.

[140]  D. Wishart,et al.  The food metabolome: a window over dietary exposure. , 2014, The American journal of clinical nutrition.

[141]  E. Fortunato,et al.  Complementary Metal Oxide Semiconductor Technology With and On Paper , 2011, Advanced materials.

[142]  R. P. Ross,et al.  Bacteriocin production: a relatively unharnessed probiotic trait? , 2016, F1000Research.

[143]  Ashraf Ahmad,et al.  Label-free detection of few copies of DNA with carbon nanotube impedance biosensors. , 2011, Angewandte Chemie.

[144]  Junya Suehiro,et al.  Selective detection of bacteria by a dielectrophoretic impedance measurement method using an antibody-immobilized electrode chip , 2006 .

[145]  Ji Won Lim,et al.  Review of Micro/Nanotechnologies for Microbial Biosensors , 2015, Front. Bioeng. Biotechnol..

[146]  Y. Wan,et al.  Graphene oxide sheet-mediated silver enhancement for application to electrochemical biosensors. , 2011, Analytical chemistry.

[147]  Zeynep Altintas,et al.  A fully automated microfluidic-based electrochemical sensor for real-time bacteria detection. , 2018, Biosensors & bioelectronics.

[148]  Jesse R. Zaneveld,et al.  Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences , 2013, Nature Biotechnology.

[149]  Michael C. McAlpine,et al.  Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides , 2010, Proceedings of the National Academy of Sciences.

[150]  Sung‐Yool Choi,et al.  An electrochemically reduced graphene oxide-based electrochemical immunosensing platform for ultrasensitive antigen detection. , 2012, Analytical chemistry.

[151]  Mark C. Thompson,et al.  Surface-functionalization of PDMS for potential micro-bioreactor and embryonic stem cell culture applications. , 2013, Journal of materials chemistry. B.

[152]  Tae-Joon Jeon,et al.  A nanoporous membrane-based impedimetric immunosensor for label-free detection of pathogenic bacteria in whole milk. , 2013, Biosensors & bioelectronics.

[153]  G. Demirel,et al.  New Generation Biosensors based on Ellipsometry , 2011 .

[154]  A. Alessandrini,et al.  Chemically homogeneous, silylated surface for effective DNA binding and hybridization , 2005 .

[155]  P. Elizaquível,et al.  Recent developments in the use of viability dyes and quantitative PCR in the food microbiology field , 2014, Journal of applied microbiology.

[156]  Jeong-O Lee,et al.  Detection and titer estimation of Escherichia coli using aptamer-functionalized single-walled carbon-nanotube field-effect transistors. , 2008, Small.

[157]  A. Mansur,et al.  Chemical functionalization of surfaces for building three-dimensional engineered biosensors , 2013 .

[158]  Widespread amplification of amplified fragment length polymorphisms (AFLPs) in marine Antarctic animals , 2012, Polar Biology.

[159]  C. Taborda,et al.  Development of enzyme immunoassays (ELISA and Western blot) for the serological diagnosis of dermatophytosis in symptomatic and asymptomatic cats , 2018, Medical mycology.

[160]  N. Uria,et al.  Impedimetric Sensors for Bacteria Detection , 2015 .

[161]  E. Ghafar-Zadeh,et al.  CMOS capactive sensor system for bacteria detection using phage organisms , 2008, 2008 Canadian Conference on Electrical and Computer Engineering.

[162]  N. Cameron,et al.  3D Surface Functionalization of Emulsion-Templated Polymeric Foams , 2014 .

[163]  Sean R. Davis,et al.  NCBI GEO: archive for functional genomics data sets—update , 2012, Nucleic Acids Res..