Multilocus Genotyping of Giardia duodenalis in Dairy Cattle in Henan, China

Giardia duodenalis is a common and widespread intestinal protozoan parasite of both humans and animals. Previous epidemiological and molecular studies have identified Giardia infections in different animals and humans, but only limited information is available about the occurrence and genotypes of Giardia in cattle in China. In this study, we determined the occurrence of giardiasis and genetically characterized G. duodenalis in dairy cattle in Henan Province, central China. The overall prevalence of G. duodenalis was 7.2% (128/1777) on microscopic analysis, with the highest infection rate (22.7%) in calves aged less than 1 month. G. duodenalis assemblages and subtypes were identified with multilocus genotyping based on the SSU rRNA, β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes. Two assemblages were detected in the successfully sequenced samples: assemblage A (n = 58), assemblage E (n = 21), with a mixed E and A assemblage (n = 2). Four novel subtypes of the gdh gene and seven of the bg gene were found among the G. duodenalis assemblage E isolates. Using the nomenclature for the multilocus genotype (MLG) model, nine novel multilocus genotypes E (MLGs E1–E9) and three MLGs A (a novel subtype AI, previously detected subtype AII-1, and a combination of both) were identified. MLG AII-1 identified in this study may be an important zoonotic subtype. The dairy cattle in Henan are a potential public health concern.

[1]  Lihua Xiao,et al.  Occurrence of human-pathogenic Enterocytozoon bieneusi, Giardia duodenalis and Cryptosporidium genotypes in laboratory macaques in Guangxi, China. , 2014, Parasitology international.

[2]  Lihua Xiao,et al.  Molecular characterization of Giardia duodenalis isolates from police and farm dogs in China. , 2013, Experimental parasitology.

[3]  M. Guo,et al.  Concurrent Infections of Giardia duodenalis, Enterocytozoon bieneusi, and Clostridium difficile in Children during a Cryptosporidiosis Outbreak in a Pediatric Hospital in China , 2013, PLoS neglected tropical diseases.

[4]  M. Dea-Ayuela,et al.  Multilocus genotyping of Giardia duodenalis in lambs from Spain reveals a high heterogeneity. , 2012, Research in veterinary science.

[5]  M. Guo,et al.  Anthroponotic Enteric Parasites in Monkeys in Public Park, China , 2012, Emerging infectious diseases.

[6]  Haibo Zhu,et al.  Genotype identification and prevalence of Giardia duodenalis in pet dogs of Guangzhou, Southern China. , 2012, Veterinary parasitology.

[7]  Jianping Cao,et al.  Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission , 2012, PLoS neglected tropical diseases.

[8]  Yung-Fu Chang,et al.  Prevalence of Giardia duodenalis assemblages among dairy herds in the New York City Watershed. , 2012, Veterinary parasitology.

[9]  Longxian Zhang,et al.  Occurrence of bovine giardiasis and endemic genetic characterization of Giardia duodenalis isolates in Heilongjiang Province, in the Northeast of China , 2012, Parasitology Research.

[10]  Jianping Cao,et al.  Cryptosporidium cuniculus and Giardia duodenalis in Rabbits: Genetic Diversity and Possible Zoonotic Transmission , 2012, PloS one.

[11]  J. Andersson,et al.  Multilocus Genotyping of Human Giardia Isolates Suggests Limited Zoonotic Transmission and Association between Assemblage B and Flatulence in Children , 2011, PLoS neglected tropical diseases.

[12]  M. Sogin,et al.  The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems. , 2010, International journal for parasitology.

[13]  J. Andersson,et al.  From mouse to moose: multilocus genotyping of Giardia isolates from various animal species. , 2010, Veterinary parasitology.

[14]  H. Sprong,et al.  Identification of Zoonotic Genotypes of Giardia duodenalis , 2009, PLoS neglected tropical diseases.

[15]  R. Fayer,et al.  A longitudinal study of Giardia duodenalis genotypes in dairy cows from birth to 2 years of age. , 2009, Veterinary parasitology.

[16]  M. Lalle,et al.  Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. , 2008, International journal for parasitology.

[17]  R. Fayer,et al.  Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. , 2008, International journal for parasitology.

[18]  C. Townsend,et al.  Molecular Characterization of Giardia Isolates from Calves and Humans in a Region in Which Dairy Farming Has Recently Intensified , 2008, Applied and Environmental Microbiology.

[19]  J. Vercruysse,et al.  Parasitic infections in dairy cattle around Hanoi, northern Vietnam. , 2008, Veterinary parasitology.

[20]  D. Berkvens,et al.  Mixed Giardia duodenalis assemblage A and E infections in calves. , 2008, International journal for parasitology.

[21]  C. Townsend,et al.  Prevalence of Giardia and Cryptosporidium spp in calves from a region in New Zealand experiencing intensifi cation of dairying , 2008, New Zealand veterinary journal.

[22]  J. Farber,et al.  Prevalence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in dairy cattle in Ontario, Canada. , 2007, Veterinary parasitology.

[23]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[24]  A. Castro,et al.  Molecular characterization of Cryptosporidium and Giardia isolates from cattle from Portugal. , 2007, Veterinary parasitology.

[25]  H. Vigre,et al.  Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark , 2006, Parasitology.

[26]  M. Mezo,et al.  Prevalence and intensity of infection of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Galicia (NW Spain). , 2006, Journal of veterinary medicine. B, Infectious diseases and veterinary public health.

[27]  A. Tait,et al.  Tools for investigating the environmental transmission of Cryptosporidium and Giardia infections in humans. , 2006, Trends in parasitology.

[28]  R. Fayer,et al.  Prevalence and genotypes of Giardia duodenalis in post-weaned dairy calves. , 2005, Veterinary parasitology.

[29]  D. Crotti,et al.  Genetic heterogeneity at the beta-giardin locus among human and animal isolates of Giardiaduodenalis and identification of potentially zoonotic subgenotypes. , 2005, International journal for parasitology.

[30]  R C Andrew Thompson,et al.  The zoonotic significance and molecular epidemiology of Giardia and giardiasis. , 2004, Veterinary parasitology.

[31]  R. Fayer,et al.  Prevalence of Giardia duodenalis genotypes in pre-weaned dairy calves. , 2004, Veterinary parasitology.

[32]  I. Robertson,et al.  Molecular epidemiology of Giardia and Cryptosporidium infections in dairy calves originating from three sources in Western Australia. , 2004, Veterinary parasitology.

[33]  M. Nei,et al.  Prospects for inferring very large phylogenies by using the neighbor-joining method. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[34]  R. Fayer,et al.  Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. , 2004, Veterinary parasitology.

[35]  Paul Monis,et al.  Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. , 2004, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[36]  R. Gilman,et al.  Triosephosphate Isomerase Gene Characterization and Potential Zoonotic Transmission of Giardia duodenalis , 2003, Emerging infectious diseases.

[37]  G. Mayrhofer,et al.  Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. , 2003, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[38]  M. Olson,et al.  Prevalence and genotyping of Giardia duodenalis from beef calves in Alberta, Canada. , 2003, Veterinary parasitology.

[39]  E. Pozio,et al.  Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. , 2002, International journal for parasitology.

[40]  J. Noordhuizen,et al.  Epidemiology of Cryptosporidium spp. and Giardia duodenalis on a dairy farm. , 2001, Veterinary parasitology.

[41]  H. Mohammed,et al.  Prevalence of Giardia sp. Cryptosporidium parvum and Cryptosporidium andersoni (syn. C. muris) [correction of Cryptosporidium parvum and Cryptosporidium muris (C. andersoni)] in 109 dairy herds in five counties of southeastern New York. , 2000, Veterinary parasitology.

[42]  H. Mohammed,et al.  Epidemiologic study of Giardia sp. infection in dairy cattle in southeastern New York State. , 2000, Veterinary parasitology.

[43]  D. Morck,et al.  Duration of naturally acquired giardiosis and cryptosporidiosis in dairy calves and their association with diarrhea. , 1999, Journal of the American Veterinary Medical Association.

[44]  J. Quílez,et al.  Prevalence of Cryptosporidium and Giardia infections in cattle in Aragón (northeastern Spain) , 1996, Veterinary Parasitology.

[45]  G. Mayrhofer,et al.  Division of Giardia isolates from humans into two genetically distinct assemblages by electrophoretic analysis of enzymes encoded at 27 loci and comparison with Giardia muris , 1995, Parasitology.

[46]  Longxian Zhang,et al.  Genetic characterizations of Cryptosporidium spp. and Giardia duodenalis in humans in Henan, China. , 2011, Experimental parasitology.

[47]  J. Vercruysse,et al.  Is Giardia a significant pathogen in production animals? , 2010, Experimental parasitology.