Biocompatibility Testing on Cell Culture of some Root Canal Sealers used in Endodontics

Cleaning and shaping of the root canal, followed by a complete seal are an important steps in root canal treatment. The aim of this study was a comparative evaluation of biocompatibility of some endodontic root-end filling materials on cultures of primary human osteoblasts (OBL) and dental follicle derived adult mesenchymal stem cells (DF MSCs). The differentiation process was evaluated with alamar blue viability assay and by immunocytochemical staining for bone markers (osteopontin, alkaline phosphates). The samples were examined using a Zeiss Axiovert microscope by reversed phase fluorescence and image capture was performed using a MRC camera. Tested materials proved to develop a degree of toxicity that occurs especially when osteoblasts after 2 weeks, with higher extent for AH Plus material. A very good biocompatibility with stem cells was observed for Acroseal especially after 9 days of cultivation, with an increased proliferation rate of DF MSCs.