Isolation and characterization of rice MADS box gene homologues and their RFLP mapping.

Thirty-five MADS box gene homologues were identified through a large-scale cDNA analysis in rice. Based on the nucleotide sequences of the 3'-untranslated region, these clones were classified into 11 independent species. Seven species were found to be new among the rice MADS box gene family, and the other 4 corresponded to the previously reported OsMADS1, OsMADS2, OsMADS4, and OsMADS5. The full nucleotide sequences of the 7 new species were determined. Each clone encoded a deduced protein of 164-267 amino acids. The K-domain of the MADS protein was conserved in all clones though with lower degree in clone S10304. Reverse transcription PCR analysis showed that clones E31254 and E31864 were expressed mainly in panicles. Dendrogram analysis suggested that E31254 and E31864 are close to Arabidopsis AGL9 and AP1, respectively. Restriction fragment length polymorphism (RFLP) linkage mapping revealed that the rice MADS box gene homologues reported here are not clustered but are located throughout the genome. The locus of E31864 on the RFLP map was closely linked to the long sterile lemma gene, g-1.

[1]  K. Devos,et al.  Comparative genetics in the grasses. , 1998, Plant molecular biology.

[2]  G. An,et al.  Characterization of two rice MADS box genes that control flowering time. , 1997, Molecules and cells.

[3]  M. E. Pè,et al.  MADS box genes expressed in developing inflorescences of rice and sorghum , 1997, Molecular and General Genetics MGG.

[4]  G. An,et al.  Isolation and characterization of a rice MADS box gene belonging to the AGL2 gene family. , 1997, Molecules and cells.

[5]  H. Sommer,et al.  Multiple interactions amongst floral homeotic MADS box proteins. , 1996, The EMBO journal.

[6]  G. Ditta,et al.  Diverse roles for MADS box genes in Arabidopsis development. , 1995, The Plant cell.

[7]  G. An,et al.  Characterization of two rice MADS box genes homologous to GLOBOSA , 1995 .

[8]  F. Salamini,et al.  ZEMa, a member of a novel group of MADS box genes, is alternatively spliced in maize endosperm. , 1995, Nucleic acids research.

[9]  M. Purugganan,et al.  Molecular evolution of flower development: diversification of the plant MADS-box regulatory gene family. , 1995, Genetics.

[10]  H. Saedler,et al.  Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize. , 1995, Gene.

[11]  S. Lin,et al.  A 300 kilobase interval genetic map of rice including 883 expressed sequences , 1994, Nature Genetics.

[12]  Elliot M. Meyerowitz,et al.  The ABCs of floral homeotic genes , 1994, Cell.

[13]  Cindy Gustafson-Brown,et al.  Regulation of the arabidopsis floral homeotic gene APETALA1 , 1994, Cell.

[14]  S. Hake,et al.  Identification and molecular characterization of ZAG1, the maize homolog of the Arabidopsis floral homeotic gene AGAMOUS. , 1993, The Plant cell.

[15]  H. Sommer,et al.  GLOBOSA: a homeotic gene which interacts with DEFICIENS in the control of Antirrhinum floral organogenesis. , 1992, The EMBO journal.

[16]  G. Angenent,et al.  Differential expression of two MADS box genes in wild-type and mutant petunia flowers. , 1992, The Plant cell.

[17]  E. Meyerowitz,et al.  AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. , 1991, Genes & development.

[18]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[19]  Hong Ma,et al.  The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors , 1990, Nature.

[20]  H. Sommer,et al.  Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors. , 1990, The EMBO journal.

[21]  Y. Iwasaki,et al.  Cell-free synthesis of succinate dehydrogenase and mitochondrial adenosine triphosphatase of sweet potato. , 1983, Biochemical and biophysical research communications.

[22]  P. Y. Chou,et al.  Prediction of protein conformation. , 1974, Biochemistry.

[23]  N. Iwata,et al.  Linkage Analysis by Reciprocal Translocation Method in Rice Plants (Oryza Sativa L.). : I. Linkage Groups Corresponding to the Chromosome 1, 2, 3 and 4 , 1971 .

[24]  Man-emon Takahashi,et al.  Trial Construction of Twelve Linkage Groups in Japanese Rice : (Genetical Studies on Rice Plant, XXVII） , 1963 .

[25]  M. Takahashi,et al.  Genetical studies on rice plant, XXVII. Trial construction of twelve linkage groups in Japanese rice , 1963 .

[26]  M. Yano,et al.  Isolation and mapping of a family of putative zinc-finger protein cDNAs from rice. , 1998, DNA research : an international journal for rapid publication of reports on genes and genomes.

[27]  S. Lin,et al.  A high-density rice genetic linkage map with 2275 markers using a single F2 population. , 1998, Genetics.

[28]  M. Yano,et al.  Rice class III chitinase homologues isolated by random cloning of rice cDNAs. , 1997, DNA research : an international journal for rapid publication of reports on genes and genomes.

[29]  M. Yano,et al.  Characterization and mapping of cDNA encoding aspartate aminotransferase in rice, Oryza sativa L. , 1996, DNA research : an international journal for rapid publication of reports on genes and genomes.

[30]  大村 武,et al.  Linkage Study of the Genes Belonging to the First Chromosome in Rice , 1962 .