3D resolved mapping of optical aberrations in thick tissues

We demonstrate a simple method for mapping optical aberrations with 3D resolution within thick samples. The method relies on the local measurement of the variation in image quality with externally applied aberrations. We discuss the accuracy of the method as a function of the signal strength and of the aberration amplitude and we derive the achievable resolution for the resulting measurements. We then report on measured 3D aberration maps in human skin biopsies and mouse brain slices. From these data, we analyse the consequences of tissue structure and refractive index distribution on aberrations and imaging depth in normal and cleared tissue samples. The aberration maps allow the estimation of the typical aplanetism region size over which aberrations can be uniformly corrected. This method and data pave the way towards efficient correction strategies for tissue imaging applications.

[1]  D. Débarre,et al.  Dynamic aberration correction for multiharmonic microscopy. , 2009, Optics letters.

[2]  Iris Riemann,et al.  High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. , 2003, Journal of biomedical optics.

[3]  Eric Betzig,et al.  Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues , 2010, Nature Methods.

[4]  Huikai Xie,et al.  Refractive index measurement of acute rat brain tissue slices using optical coherence tomography , 2012, Optics express.

[5]  T. Wilson,et al.  Adaptive optics for structured illumination microscopy. , 2008, Optics express.

[6]  A. Pena,et al.  In vivo multiphoton imaging of human skin: assessment of topical corticosteroid-induced epidermis atrophy and depigmentation. , 2012, Journal of biomedical optics.

[7]  D. Agard,et al.  Computational adaptive optics for live three-dimensional biological imaging , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[8]  S. Gigan,et al.  Brain refractive index measured in vivo with high-NA defocus-corrected full-field OCT and consequences for two-photon microscopy. , 2011, Optics express.

[9]  Emmanuel Beaurepaire,et al.  Accuracy of correction in modal sensorless adaptive optics. , 2012, Optics express.

[10]  Omar E. Olarte,et al.  Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy , 2011, Biomedical optics express.

[11]  T. Wilson,et al.  Adaptive aberration correction in a confocal microscope , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[12]  Tony Wilson,et al.  Image-based adaptive optics for two-photon microscopy. , 2009, Optics letters.

[13]  Delphine Débarre,et al.  Assessing correction accuracy in image-based adaptive optics , 2012, Photonics West - Micro and Nano Fabricated Electromechanical and Optical Components.

[14]  W. Denk,et al.  Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing , 2006, Proceedings of the National Academy of Sciences.

[15]  Atsushi Miyawaki,et al.  Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain , 2011, Nature Neuroscience.

[16]  J. Girkin,et al.  Practical implementation of adaptive optics in multiphoton microscopy. , 2003, Optics express.

[17]  R. W. Draft,et al.  Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system , 2007, Nature.

[18]  Michael Hart,et al.  Recent advances in astronomical adaptive optics. , 2010, Applied optics.

[19]  Takashi R Sato,et al.  Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex , 2011, Proceedings of the National Academy of Sciences.

[20]  P. Bourgine,et al.  Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy , 2010, Science.

[21]  Karsten König,et al.  Depth‐resolved measurement of the dermal matrix composition by multiphoton laser tomography , 2010, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.