Near-infrared fluorescence lifetime assay for serum glucose based on allophycocyanin-labeled concanavalin A.

We describe an assay scheme for glucose based on fluorescence resonance energy transfer (FRET) between concanavalin A (con A), labeled with the near-infrared fluorescent protein allophycocyanin (APC) as donor, and dextran labeled with malachite green (MG) as acceptor. Glucose competitively displaces dextran-MG and leads to reduction in FRET, assessed by time-domain fluorescence lifetime measurements using time-correlated single-photon counting. The assay is operative in the glucose concentration range 2.5-30 mM, and therefore suitable for use in monitoring diabetes control. Albumin and serum inhibit FRET but the interference can be prevented by removal of high molecular weight substances by membrane filters. APC shows promise for incorporation in an implanted glucose sensor which can be interrogated from outside the body.

[1]  O. Rolinski,et al.  A method of determining donor-acceptor distribution functions in Forster resonance energy transfer , 2000 .

[2]  O. Rolinski,et al.  Determination of acceptor distribution from fluorescence resonance energy transfer: theory and simulation , 2000 .

[3]  J C Pickup,et al.  A time-resolved near-infrared fluorescence assay for glucose: opportunities for trans-dermal sensing. , 2000, Journal of photochemistry and photobiology. B, Biology.

[4]  John Pickup,et al.  In vivo glucose sensing for diabetes management: progress towards non-invasive monitoring , 1999, BMJ.

[5]  O. Rolinski,et al.  Fluorescence resonance energy transfer from allophycocyanin to malachite green , 1999 .

[6]  G L Coté,et al.  A fluorescence-based glucose biosensor using concanavalin A and dextran encapsulated in a poly(ethylene glycol) hydrogel. , 1999, Analytical chemistry.

[7]  L. Heinemann,et al.  Non-invasive continuous glucose monitoring in Type I diabetic patients with optical glucose sensors , 1998, Diabetologia.

[8]  Joseph R. Lakowicz,et al.  Optical assay for glucose based on the luminescnence decay time of the long wavelength dye Cy5™. , 1997, Sensors and actuators. B, Chemical.

[9]  H Szmacinski,et al.  Lifetime-based sensing of glucose using energy transfer with a long lifetime donor. , 1997, Analytical biochemistry.

[10]  M A Arnold,et al.  Non-invasive glucose monitoring. , 1996, Current opinion in biotechnology.

[11]  S. Genuth,et al.  The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. , 1993, The New England journal of medicine.

[12]  H. Heise,et al.  Noninvasive Blood Glucose Assay by Near-Infrared Diffuse Reflectance Spectroscopy of the Human Inner Lip , 1993 .

[13]  J. Pickup,et al.  Developing glucose sensors for in vivo use. , 1993, Trends in biotechnology.

[14]  E. V. Thomas,et al.  Noninvasive glucose monitoring in diabetic patients: a preliminary evaluation. , 1992, Clinical chemistry.

[15]  U Fischer,et al.  Subcutaneous glucose monitoring by means of electrochemical sensors: fiction or reality? , 1992, Journal of biomedical engineering.

[16]  Marc J.P. Leiner,et al.  Luminescence chemical sensors for biomedical applications: scope and limitations , 1991 .

[17]  M. Dailey,et al.  A novel and inexpensive source of allophycocyanin for multicolor flow cytometry. , 1989, Journal of immunological methods.

[18]  J. Schultz,et al.  Fiber-optic biosensors based on fluorescence energy transfer. , 1988, Talanta.

[19]  M. Kronick,et al.  The use of phycobiliproteins as fluorescent labels in immunoassay. , 1986, Journal of immunological methods.

[20]  Y. Yamasaki,et al.  WEARABLE ARTIFICIAL ENDOCRINE PANCREAS WITH NEEDLE-TYPE GLUCOSE SENSOR , 1982, The Lancet.

[21]  L. Stryer,et al.  Fluorescent phycobiliprotein conjugates for analyses of cells and molecules , 1982, The Journal of cell biology.

[22]  J. Schultz,et al.  Affinity Sensor: A New Technique for Developing Implantable Sensors for Glucose and Other Metabolites , 1982, Diabetes Care.