Modeling of the simulated moving-bed reactor for the enzyme-catalyzed production of lactosucrose

The enzyme-catalyzed production of lactosucrose in a simulated moving-bed reactor is investigated. A numerical model is derived and verified by data obtained from simulated moving-bed reactor experiments. Based on the derived model, parameter studies and optimization are carried out. It is found that along with the flow rate settings, substrate feed and enzyme concentration and thermal deactivation of enzyme strongly influenced the product yield. Simulation showed that despite of parallel and consecutive side reaction, the maximum lactosucrose yield can reach 69%, which represents a yield increase of 36% relative to the equilibrium yield.

[1]  Kenji Yoshimoto,et al.  Increased esterification conversion by application of the simulated moving-bed reactor , 1996 .

[2]  M. Douglas LeVan,et al.  Fundamentals of Adsorption , 1996 .

[3]  Alírio E. Rodrigues,et al.  Design methodology and operation of a simulated moving bed reactor for the inversion of sucrose and glucose–fructose separation , 2001 .

[4]  K. Fujita,et al.  Transfructosylation Catalyzed by β-Fructofuranosidase I from Arthrobacter sp. K-1 , 1990 .

[5]  Yasunobu Inoue,et al.  The simulated moving-bed reactor for production of bisphenol A , 1999 .

[6]  Rutherford Aris,et al.  The simulated countercurrent moving bed chromatographic reactor: a novel reactor—separator , 1994 .

[7]  G. Ganetsos,et al.  Preparative and Production Scale Chromatography , 1992 .

[8]  C. B. Ching,et al.  Simulated Moving-Bed Reactor: Application in Bioreaction and Separation , 1997 .

[9]  K. Fujita,et al.  Purification and some properties of beta-fructofuranosidase I from Arthrobacter sp. K-1. , 1990, Agricultural and biological chemistry.

[10]  Ajay K. Ray,et al.  Experimental study of a laboratory-scale simulated countercurrent moving bed chromatographic reactor , 1995 .

[11]  M. N. Gupta.  Thermostability of enzymes , 1993 .

[12]  K. Hashimoto,et al.  A new process combining adsorption and enzyme reaction for producing higher‐fructose syrup , 1983, Biotechnology and bioengineering.

[13]  J. Frère,et al.  Quantitative analysis of the stabilization by substrate of Staphylococcus aureus PC1 beta-lactamase. , 2001, Chemistry & biology.

[14]  George T. Tsao,et al.  Recent advances in the simultaneous bioreaction and product separation processes , 1993 .

[15]  Henner Schmidt-Traub,et al.  Effect of process parameters on the performance of a simulated moving bed chromatographic reactor , 1999 .

[16]  Kenji Hashimoto,et al.  Lactosucrose production using a simulated moving bed reactor , 2001 .

[17]  J. Strube,et al.  Dynamic simulation of simulated moving bed chromatographic reactors , 1997 .

[18]  Belarbi,et al.  Kinetics of thermal deactivation of enzymes: a simple three parameters phenomenological model can describe the decay of enzyme activity, irrespectively of the mechanism. , 2000, Enzyme and microbial technology.

[19]  K. Fujita,et al.  Reaction Kinetics and Modeling of the Enzyme-catalyzed Production of Lactosucrose using β-Fructofuranosidase from Arthrobacter sp. K-1 , 2001, Bioscience, biotechnology, and biochemistry.