Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells.

Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.

[1]  H. Antoniades,et al.  Simian sarcoma virus--transformed cells secrete a mitogen identical to platelet-derived growth factor. , 1984, Science.

[2]  D. Givol,et al.  Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor , 1984, Cell.

[3]  L. Ratner,et al.  Human-proto-oncogene nucleotide sequences corresponding to the transforming region of simian sarcoma virus. , 1984, Science.

[4]  C. Heldin,et al.  Synthesis of a PDGF-like growth factor in human glioma and sarcoma cells suggests the expression of the cellular homologue to the transforming protein of simian sarcoma virus. , 1983, Biochemical and biophysical research communications.

[5]  M. Hunkapiller,et al.  Structural and immunological similarities between simian sarcoma virus gene product(s) and human platelet-derived growth factor , 1983, Nature.

[6]  R F Doolittle,et al.  Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor. , 1983, Science.

[7]  Paul Stroobant,et al.  Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus , 1983, Nature.

[8]  M. Hunkapiller,et al.  Human platelet-derived growth factor (PDGF): amino-terminal amino acid sequence. , 1983, Science.

[9]  H. Antoniades,et al.  Evidence that a human osteosarcoma cell line which secretes a mitogen similar to platelet-derived growth factor requires growth factors present in platelet-poor plasma. , 1983, Cancer research.

[10]  E. Reddy,et al.  In vivo identification of the transforming gene product of simian sarcoma virus. , 1982, Science.

[11]  M. Sporn,et al.  Synergistic interaction of two classes of transforming growth factors from murine sarcoma cells. , 1982, Cancer research.

[12]  R. Ross,et al.  Platelet-derived Growth Factor , 2022 .

[13]  J. Lautenberger,et al.  Cellular genes analogous to retroviral onc genes are transcribed in human tumour cells , 1982, Nature.

[14]  T. Deuel,et al.  Human platelet-derived growth factor. Purification and resolution into two active protein fractions. , 1981, The Journal of biological chemistry.

[15]  W. Bonner,et al.  Quantitative determination of histone modification. H2A acetylation and phosphorylation. , 1981, The Journal of biological chemistry.

[16]  C. Heldin,et al.  Chemical and biological properties of a growth factor from human‐cultured osteosarcoma cells: Resemblance with platelet‐derived growth factor , 1980, Journal of cellular physiology.

[17]  M. Sporn,et al.  Autocrine secretion and malignant transformation of cells. , 1980, The New England journal of medicine.

[18]  W. Bonner,et al.  Two-dimensional gel analysis of histones in acid extracts of nuclei, cells, and tissues. , 1980, European journal of biochemistry.

[19]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[20]  C. Heldin,et al.  Platelet-derived growth factor: purification and partial characterization. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[21]  C. Stiles,et al.  Platelet alpha granules contain a growth factor for fibroblasts. , 1979, Blood.

[22]  C. Stiles,et al.  Purification of human platelet-derived growth factor. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[23]  G. Todaro,et al.  Growth factors from murine sarcoma virus-transformed cells. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[24]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[25]  B. Westermark,et al.  Initiation of DNA synthesis of stationary human glia-like cells by a polypeptide fraction from human plasma containing somatomedin activity. , 1975, Experimental cell research.

[26]  H. Antoniades,et al.  Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[27]  A. Lipton,et al.  Platelets as a source of fibroblast growth-promoting activity. , 1974, Experimental cell research.

[28]  R. Ross,et al.  A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[29]  P. Leder,et al.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. , 1972, Proceedings of the National Academy of Sciences of the United States of America.