Changes in serum alkaline phosphatase isoenzymes associated with diabetes mellitus, fasting and ageing in the rat.

We have determined the effect of diabetes mellitus and starvation/re-feeding on serum alkaline phosphatase (ALP) isoenzymes in the rat. Both ketotic (DKA) and nonketotic (NKDM) diabetic rats showed elevated total ALP activity, primarily due to an increase in intestinal and bone/liver ALP isoenzyme in the NKDM and DKA groups, respectively. There was a decrease in intestinal ALP activity in 3 day starved rats which was reversed after re-feeding for 5 days. Aging was associated with a fall in the bone/liver isoenzyme. This latter change was mainly attributed to a fall in the “bone fraction” of the bone/liver ALP isoenzyme. Since in humans bone/liver and intestinal ALP activity is altered by diabetes mellitus and feeding may increase intestinal ALP, the rat models may be useful in the study of the variables that influence ALP.

[1]  M. Feher,et al.  Triglyceride Fatty Acid Chain Length Influences the Post Prandial Rise in Serum Intestinal Alkaline Phosphatase Activity , 1992, Annals of clinical biochemistry.

[2]  D. Mikhailidis,et al.  Haematological profile and plasma and urinary biochemistry in experimental models of diabetes mellitus: predictive power for human studies. , 1992 .

[3]  T. Kuwana,et al.  Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition. , 1991, Journal of clinical pathology.

[4]  Komoda Tsugikazu,et al.  Translocation of intestinal alkaline phosphatase in streptozotocin-induced diabetic rats , 1990 .

[5]  T. Komoda,et al.  Translocation of intestinal alkaline phosphatase in streptozotocin-induced diabetic rats. , 1990, The International journal of biochemistry.

[6]  D. Mikhailidis,et al.  Effect of starvation and sampling time on plasma alkaline phosphatase activity and calcium homeostasis in the rat , 1989, Laboratory animals.

[7]  A. Y. Foo,et al.  Lectin affinity electrophoresis of alkaline phosphatase for the differentiation of bone and hepatobiliary disease , 1989, Electrophoresis.

[8]  A. Smith,et al.  Plasma alkaline phosphatase isoenzymes in diabetes mellitus. , 1988, Clinica chimica acta; international journal of clinical chemistry.

[9]  P. Dandona,et al.  Total alkaline phosphatase and quantitative isoenzyme analysis in diabetes mellitus. , 1988 .

[10]  D. Mikhailidis,et al.  Effect of starvation on biochemical indices of renal function in the rat. , 1987, British journal of experimental pathology.

[11]  H. Estep,et al.  Serum alkaline phosphatase in diabetes mellitus. , 1986, Journal of the American College of Nutrition.

[12]  H. Schedl,et al.  Gastrin and growth of the alimentary tract in the streptozotocin-diabetic rat. , 1982, The American journal of physiology.

[13]  S. Teitelbaum,et al.  Alkaline phosphatase activity in chronic streptozotocin-induced insulin deficiency in the rat: effect of insulin replacement. , 1981, Metabolism: clinical and experimental.

[14]  R. Kreisberg,et al.  Diabetic ketoacidosis: new concepts and trends in pathogenesis and treatment. , 1978, Annals of internal medicine.

[15]  J. W. Osborne,et al.  Proliferation rate and transit time of mucosal cells in small intestine of the diabetic rat. , 1977, Gastroenterology.

[16]  E. Riecken,et al.  Structural and functional studies on the transformation of the intestinal mucosa in rats with experimental diabetes , 1977, Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie.

[17]  M. D. de Broe,et al.  L-p-Bromotetramisole, a new reagent for use in measuring placental or intestinal isoenzymes of alkaline phosphatase in human serum. , 1977, Clinical chemistry.

[18]  D. Goldberg,et al.  Elevation of serum alkaline phosphatase activity and related enzymes in diabetes mellitus. , 1977, Clinical biochemistry.

[19]  F. Belfiore,et al.  Serum enzymes in diabetes mellitus. , 1973, Clinical chemistry.

[20]  B. Highman,et al.  Blood chemistry values in normal Mystromys albicaudatus and Osborne-Mendel rats. , 1971, Laboratory animal science.

[21]  K. Isselbacher,et al.  Increased lymph alkaline phosphatase after fat feeding: effects of medium chain triglycerides and inhibition of protein synthesis. , 1970, Biochimica et biophysica acta.

[22]  N. Madsen,et al.  The relationship of dietary factors to rat serum alkaline phosphatase. II. The effect of total food consumption, methionine, choline, and vitamin B12 in normal and alloxan diabetic adult rats. , 1952, Canadian journal of medical sciences.

[23]  J. Bollman,et al.  Alkaline phosphatase in the intestinal lymph of the rat. , 1948, The Journal of biological chemistry.

[24]  J. Tuba,et al.  Serum Phosphatases and Alloxan Diabetes. , 1947, Science.

[25]  L. Weil,et al.  STUDIES ON PLASMA PHOSPHATASE ACTIVITY IN RELATION TO FAT METABOLISM IN RATS , 1940 .