Molecular X-ray computed tomography of myelin in a rat brain

In this work we demonstrate the feasibility of applying small-angle X-ray scattering computed tomography (SAXS-CT) for non-invasive molecular imaging of myelin sheaths in a rat brain. Our results show that the approach yields information on several quantities, including the relative myelin concentration, its periodicity, the total thickness of the myelin sheaths, and the relative concentration of cytoskeletal neurofilaments. For example the periodicity of the myelin sheaths varied in the range from 17.0 to 18.2 nm around an average of 17.6 (±0.3) nm. We believe that imaging, i.e., spatially resolved measuring these quantities could provide general means for understanding the relation to a number of neurodegenerative diseases.

[1]  J. Kosanetzky,et al.  Scattered X-ray beam nondestructive testing , 1989 .

[2]  J. Prineas,et al.  Pathology of Multiple Sclerosis , 2001 .

[3]  I. Robinson.  Elements of Modern X-ray Physics , 2002 .

[4]  George Bartzokis,et al.  Quadratic trajectories of brain myelin content: unifying construct for neuropsychiatric disorders , 2004, Neurobiology of Aging.

[5]  Franz Pfeiffer,et al.  Multimodal x-ray scatter imaging , 2009 .

[6]  W Freysinger,et al.  High‐resolution X‐ray tomography of the human inner ear: synchrotron radiation‐based study of nerve fibre bundles, membranes and ganglion cells , 2009, Journal of microscopy.

[7]  Paul Roschger,et al.  Position-Resolved Small-Angle X-ray Scattering of Complex Biological Materials , 1997 .

[8]  M. Smith,et al.  Neurofilament proteins in neurodegenerative diseases , 2004, Cellular and Molecular Life Sciences CMLS.

[9]  I. Janota,et al.  Cerebral malaria in the United Kingdom. , 1979, Journal of clinical pathology.

[10]  P. Fratzl,et al.  Scanning Small Angle X-ray Scattering Analysis of Human Bone Sections , 1999, Calcified Tissue International.

[11]  Ludwin Sk,et al.  The neuropathology of multiple sclerosis. , 2000 .

[12]  Avinash C. Kak,et al.  Principles of computerized tomographic imaging , 2001, Classics in applied mathematics.

[13]  F De Carlo,et al.  High energy X-ray scattering tomography applied to bone. , 2008, Journal of structural biology.

[14]  U Neitzel,et al.  X-ray diffraction computed tomography. , 1987, Medical physics.

[15]  U Karlsson,et al.  Comparison of the myelin period of peripheral and central origin by electron microscopy. , 1966, Journal of ultrastructure research.

[16]  P Suortti,et al.  Feasibility study of x-ray diffraction computed tomography for medical imaging. , 1998, Physics in medicine and biology.

[17]  P. Paez,et al.  Morphological Changes of Myelin Sheaths in Rats Intracranially Injected with Apotransferrin , 2004, Neurochemical Research.

[18]  P. Fratzl,et al.  Analysis of the hierarchical structure of biological tissues by scanning X-ray scattering using a micro-beam. , 2000, Cellular and molecular biology.

[19]  A. Peters,et al.  Rapid assessment of internodal myelin integrity in central nervous system tissue , 2009, Journal of neuroscience research.

[20]  Kevin C. Chan,et al.  B-value dependence of DTI quantitation and sensitivity in detecting neural tissue changes , 2010, NeuroImage.

[21]  Shawn Mikula,et al.  Internet-enabled high-resolution brain mapping and virtual microscopy , 2007, NeuroImage.

[22]  C. Bonifazzi,et al.  Biological tissue characterisation in narrow beam x-ray scattering techniques , 2009 .

[23]  F. Sjöstrand.  The lamellated structure of the nerve myelin sheath as revealed by high resolution electron microscopy , 1953, Experientia.

[24]  J. Schröder,et al.  Neuropathology of Charcot-Marie-Tooth and related disorders , 2007, NeuroMolecular Medicine.

[25]  Christian G. Schroer,et al.  Mapping the local nanostructure inside a specimen by tomographic small-angle x-ray scattering , 2006 .

[26]  Alberto Bravin,et al.  Human breast cancer in vitro: matching histo-pathology with small-angle x-ray scattering and diffraction enhanced x-ray imaging , 2005, Physics in medicine and biology.

[27]  Roberto Dinapoli,et al.  PILATUS: A single photon counting pixel detector for X-ray applications , 2009 .

[28]  U Neitzel,et al.  Elastic scatter computed tomography. , 1985, Physics in medicine and biology.

[29]  M Gambaccini,et al.  Structural characterization of the human cerebral myelin sheath by small angle x-ray scattering , 2008, Physics in medicine and biology.

[30]  M. Burghammer,et al.  Scanning X-ray imaging with small-angle scattering contrast , 2007 .

[31]  R. Rudick,et al.  Axonal transection in the lesions of multiple sclerosis. , 1998, The New England journal of medicine.

[32]  B. Trapp,et al.  Transected neurites, apoptotic neurons, and reduced inflammation in cortical multiple sclerosis lesions , 2001, Annals of neurology.