Synthesis of protein-nucleic acid conjugates by expressed protein ligation.

The synthesis of covalent conjugates of proteins and polyamide nucleic acids (PNA) is accomplished by expressed protein ligation of intein-fusion proteins and a PNA-cysteine conjugate.

[1]  Dieter Stoll,et al.  Protein microarray technology. , 2002, Frontiers in bioscience : a journal and virtual library.

[2]  Tom W Muir,et al.  Recent advances in the application of expressed protein ligation to protein engineering. , 2002, Current opinion in biotechnology.

[3]  M. Gait,et al.  Efficient conjugation of peptides to oligonucleotides by "native ligation". , 2000, The Journal of organic chemistry.

[4]  H. Hansen,et al.  Improvements in automated PNA synthesis using Boc/Z monomers. , 2009, The journal of peptide research : official journal of the American Peptide Society.

[5]  C R Cantor,et al.  Oligonucleotide-directed self-assembly of proteins: semisynthetic DNA--streptavidin hybrid molecules as connectors for the generation of macroscopic arrays and the construction of supramolecular bioconjugates. , 1994, Nucleic acids research.

[6]  C. Niemeyer.  The developments of semisynthetic DNA-protein conjugates. , 2002, Trends in biotechnology.

[7]  R. Goody,et al.  Kinetics of interaction of nucleotides with nucleotide-free H-ras p21. , 1990, Biochemistry.

[8]  Peter G Schultz,et al.  From Split-Pool Libraries to Spatially Addressable Microarrays and Its Application to Functional Proteomic Profiling. , 2001, Angewandte Chemie.

[9]  D. Filippov,et al.  An approach to the synthesis of peptide–PNA–peptide conjugates via native ligation , 2002 .

[10]  C. Niemeyer,et al.  DNA-Directed immobilization: efficient, reversible, and site-selective surface binding of proteins by means of covalent DNA-streptavidin conjugates. , 1999, Analytical biochemistry.

[11]  S. Stein,et al.  Preparation and applications of peptide-oligonucleotide conjugates. , 2000, Bioconjugate chemistry.