The Skin Whitening Effect of Gypsum Extract using B16F10 cell lines

Objectives : Recently the aesthetic, especially skin whitening, is focused on. This study was designed to investigate the effects of Gypsum Extract(GE) on skin whitening using B16F10 cell lines. Methods : In this study, we investigated effect of GE on cell viability, melanin synthesis, Superoxide dismutase(SOD)-like activity and tyrosinase activity in vitro and in vivo. Results : In results, The proliferation of B16F10 cell lines was increased by GE concentration. But there was no statistical signification. Melanin synthesis was decreased except GE 125μg/ml of treated group. And the melanin synthesis ratio was increased in group being treated α-MSH. But After treating GE, it was decreased compared to Non-GE treated group. In addition, the SOD-like activity was enhanced in more than GE 125μg/ml of treated group. And the activity of tyrosinase in vitro was inhibited in GE 500μg/ml and 1,000μg/ml of treated groups. The activity of tyrosinase in vivo was reduced in both Arbutin 500μg/ml of treated group and Gypsum 500μg/ml of treated group compared to controled group. After being treated α-MSH, the activity of tyrosinase was enhanced. But after treating Arbutin 500μg/ml or Gypsum 500μg/ml, it was inhibited being compared to the group of Non-medicament treated. Conclusion : The results of this study showed the effects of GE that can inhibit melanin synthesis and the activity of tyrosinase and enhance the SOD-like activity. It means that gypsum has the skin whitening effect.