Precise patterning of biopolymers and cells by direct write technique

Abstract It is demonstrated how micro-periodic patterns can be precisely created using biopolymer solutions of optimised rheological properties. Direct-write assembly enabled the construction of micro-periodic structures without the need for expensive tooling, dies, or lithographic masks. It is reported that rationalised designer patterns of defined filamentous topography could provide contact guidance effect for alignment of corneal stromal cells and dermal fibroblasts, and control cellular morphology to simulate organisation of cells and extracellular matrix in native tissues.

[1]  David T Corr,et al.  Gelatin-based laser direct-write technique for the precise spatial patterning of cells. , 2011, Tissue engineering. Part C, Methods.

[2]  David L. Kaplan,et al.  Direct‐Write Assembly of Microperiodic Silk Fibroin Scaffolds for Tissue Engineering Applications , 2008 .

[3]  Sanskrita Das,et al.  Enhanced redifferentiation of chondrocytes on microperiodic silk/gelatin scaffolds: toward tailor-made tissue engineering. , 2013, Biomacromolecules.

[4]  Howard Y. Chang,et al.  Anatomic Demarcation of Cells: Genes to Patterns , 2009, Science.

[5]  Y. Ito,et al.  Surface micropatterning to regulate cell functions. , 1999, Biomaterials.

[6]  C. Luo,et al.  Fabrication of gelatin nanopatterns for cell culture studies , 2013 .

[7]  Matthias P. Lutolf,et al.  Designing materials to direct stem-cell fate , 2009, Nature.

[8]  J. Lewis,et al.  Microperiodic structures: Direct writing of three-dimensional webs , 2004, Nature.

[9]  J. Paterson,et al.  Why are gelatin solutions Newtonian , 1998 .

[10]  Bruno Leuenberger,et al.  Investigation of viscosity and gelation properties of different mammalian and fish gelatins , 1991 .

[11]  D. Kaplan,et al.  In vitro model of mesenchymal condensation during chondrogenic development. , 2009, Biomaterials.

[12]  C. Grigoropoulos,et al.  Chemical patterning of ultrathin polymer films by direct-write multiphoton lithography. , 2011, Journal of the American Chemical Society.

[13]  M. Stevens,et al.  Hybrid processes in enzymatically gelled gelatin: impact on , macroscopic properties and cellular response. , 2013, Soft matter.

[14]  X. Mo,et al.  Fabrication of gelatin-hyaluronic acid hybrid scaffolds with tunable porous structures for soft tissue engineering. , 2011, International journal of biological macromolecules.

[15]  Alan Brown,et al.  Patterned cell culture substrates created by hot embossing of tissue culture treated polystyrene , 2013, Journal of Materials Science: Materials in Medicine.

[16]  J. Cesarano,et al.  Directed colloidal assembly of 3D periodic structures , 2002 .