Efficacy of three different laser wavelengths for in vitro wound healing

Background and objective: Despite contradictory reports on the effect of laser light on cell proliferation, studies have shown that appropriate doses and wavelengths of laser light are therapeutically beneficial in tissue repair and pain control. This study aimed to establish if the dose and/or wavelength influenced the biological responses of irradiated in vitro fibroblasts – 1 h after laser irradiation.

[1]  H. Abrahamse,et al.  How Long After Laser Irradiation Should Cellular Responses be Measured to Determine the Laser Effect , 2007 .

[2]  P. Whittaker,et al.  Ventricular remodeling after acute myocardial infarction: Effect of low‐intensity laser irradiation , 2000, Lasers in surgery and medicine.

[3]  H. Abrahamse,et al.  Irradiation with a 632.8 nm helium-neon laser with 5 J/cm2 stimulates proliferation and expression of interleukin-6 in diabetic wounded fibroblast cells. , 2007, Diabetes technology & therapeutics.

[4]  N. Thornberry,et al.  Caspases: killer proteases. , 1997, Trends in biochemical sciences.

[5]  Márcia Martins Marques,et al.  Effect of low‐power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts , 2002, Lasers in surgery and medicine.

[6]  T. Karu,et al.  Exact action spectra for cellular responses relevant to phototherapy. , 2005, Photomedicine and laser surgery.

[7]  A. Schindl,et al.  Low-intensity laser therapy: a review. , 2000, Journal of investigative medicine : the official publication of the American Federation for Clinical Research.

[8]  J. Rigau,et al.  Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence * , 2001, Lasers in surgery and medicine.

[9]  J. Shay,et al.  A reproducible laser-wounded skin equivalent model to study the effects of aging in vitro. , 2004, Rejuvenation research.

[10]  Nicolette N. Houreld,et al.  Effect of wavelength and fluence on morphology cellular and genetic integrity of diabetic wounded human skin fibroblasts , 2006, SPIE BiOS.

[11]  M. Marques,et al.  Influence of different power densities of LILT on cultured human fibroblast growth , 2006, Lasers in Medical Science.

[12]  Raymond J. Lanzafame,et al.  Low Level Laser Therapy: Clinical Practice and Scientific Background , 1999 .

[13]  Rizwan U. Farooqui,et al.  Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement , 2005, Journal of Cell Science.

[14]  H. Abrahamse,et al.  The release of interleukin-6 after Low Level Laser Therapy (LLLT) and the effect on migration and proliferation of human skin fibroblasts - an in vitro study , 2004 .

[15]  J. Basford,et al.  Low intensity laser therapy: Still not an established clinical tool , 1995, Lasers in surgery and medicine.

[16]  Michael R. Hamblin,et al.  Mechanisms of low level light therapy , 2006, SPIE BiOS.

[17]  Tiina I. Karu,et al.  The science of low-power laser therapy , 1998 .

[18]  T. Karu,et al.  [Molecular mechanism of the therapeutic effect of low-intensity laser irradiation]. , 1986, Doklady Akademii nauk SSSR.

[19]  J. Van Snick.  Interleukin-6: an overview. , 1990, Annual review of immunology.

[20]  B. Kennedy,et al.  Hydroxyurea inhibition of DNA synthesis in ascites tumor. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[21]  Y. Saga,et al.  Suppression of cell migration in ovarian cancer cells mediated by PTEN overexpression. , 2003, International journal of oncology.

[22]  G. Alpaslan,et al.  Extracellular alkaline phosphatase activity as a possible marker for wound healing: a preliminary report. , 1997, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[23]  B. Majaron,et al.  Novel model for evaluation of epidermal preservation and dermal collagen remodeling following photorejuvenation of human skin , 2003, Lasers in surgery and medicine.

[24]  G. Priestley,et al.  Failure of a helium‐neon laser to affect components of wound healing in vitro , 1989, The British journal of dermatology.

[25]  L O Henderson,et al.  Quantitative differences among various proteins as blocking agents for ELISA microtiter plates. , 1987, Journal of immunological methods.

[26]  R. Kozłowski,et al.  The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. , 1993, Journal of immunological methods.

[27]  H. Abrahamse,et al.  Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad-Spectrum or Infrared Light , 2007 .

[28]  Michael W. Berns,et al.  Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture , 1996, European Conference on Biomedical Optics.

[29]  Y. Aoki,et al.  Studies on mRNA expression of basic fibroblast growth factor in wound healing for wound age determination , 2003, International Journal of Legal Medicine.

[30]  A. Pinheiro,et al.  Effects of low-level laser therapy on malignant cells: in vitro study. , 2002, Journal of clinical laser medicine & surgery.

[31]  T. Vicsek,et al.  Proliferative and migratory responses of astrocytes to in vitro injury , 2000, Journal of neuroscience research.

[32]  Henrik Loevschall,et al.  Effect of low level diode laser irradiation of human oral mucosa fibroblasts in vitro , 1994, Lasers in surgery and medicine.

[33]  J. Naim,et al.  Effects of photostimulation on wound healing in diabetic mice , 1997, Lasers in surgery and medicine.

[34]  H. Abrahamse,et al.  Effect of multiple exposures of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. , 2006, Photomedicine and laser surgery.

[35]  Heidi Abrahamse,et al.  The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium‐neon laser irradiation , 2006, Lasers in surgery and medicine.

[36]  T. Mitchison,et al.  A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods , 2004, BMC biotechnology.

[37]  T Abe,et al.  Extracellular matrix regulates induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts , 2001, Journal of cellular physiology.

[38]  T. Taniguchi,et al.  Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin , 1986, Nature.