Poly(ε-caprolactone)/graphene oxide biocomposites: mechanical properties and bioactivity

Biomedical applications of graphene have recently attracted intensive attention, with graphene-based nanomaterials being reported as promising candidates in, for example, drug delivery, biosensing and bioimaging. In this paper, mechanical properties and bioactivity of nanofibrous and porous membranes electrospun from graphene oxide (GO) nanoplatelets reinforced poly(ε-caprolactone) (PCL) were investigated. The results showed that the presence of 0.3 wt% GO increased the tensile strength, modulus and energy at break of the PCL membrane by 95%, 66% and 416%, respectively, while improving its bioactivity during biomineralization and maintaining the high porosity of over 94%. The mechanical enhancements were ascribed to the change in the fiber morphology and the reinforcing effect of GO on PCL nanofibers, whereas the improvements on the bioactivity stemmed from the anionic functional groups present on the GO surface that nucleated the formation of biominerals. Systematic studies on the PCL/GO nanocomposite films with varying GO concentrations revealed that the reinforcing effect of GO on PCL was due to the strong interfacial interactions between the two phases characterized by Fourier transform infrared spectroscopy, the good dispersion of GO in the matrix and the intrinsic properties of GO nanoplatelets. The strong and bioactive PCL/GO nanofibrous membranes with a high porosity have great potential for biomedical applications.

[1]  M. Frydrych,et al.  Strong and bioactive gelatin–graphene oxide nanocomposites , 2011 .

[2]  Xiaohong Li,et al.  Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. , 2008, Biomacromolecules.

[3]  The Effect of Nanoparticle Shape on Polymer-Nanocomposite Rheology and Tensile Strength , 2007, cond-mat/0701607.

[4]  J. Feijen,et al.  Preparation of degradable porous structures based on 1,3-trimethylene carbonate and D,L-lactide (co)polymers for heart tissue engineering. , 2003, Tissue engineering.

[5]  Bin Ding,et al.  Electrospun nanomaterials for ultrasensitive sensors , 2010, Materials Today.

[6]  Yufeng Zheng,et al.  Fabrication and characterization of three-dimensional nanofiber membrance of PCL-MWCNTs by electrospinning , 2010 .

[7]  L. Ghasemi‐Mobarakeh,et al.  Electrospun poly(epsilon-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. , 2008, Biomaterials.

[8]  T. Shimanouchi.  Tables of Molecular Vibrational Frequencies Part 5 , 1972 .

[9]  Y. Inoue,et al.  Biodegradable blends of poly(ε-caprolactone) with α-chitin and chitosan: specific interactions, thermal properties and crystallization behavior , 2002 .

[10]  Y. Inoue,et al.  Thermal properties and crystallization behaviour of blends of poly(ε‐caprolactone) with chitin and chitosan , 2003 .

[11]  M. Kotaki,et al.  Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers. , 2005, Biomaterials.

[12]  David G Simpson,et al.  Nanofiber technology: designing the next generation of tissue engineering scaffolds. , 2007, Advanced drug delivery reviews.

[13]  G. Wallace,et al.  Mechanically Strong, Electrically Conductive, and Biocompatible Graphene Paper , 2008 .

[14]  V. Guarino,et al.  Influence of gelatin cues in PCL electrospun membranes on nerve outgrowth. , 2010, Biomacromolecules.

[15]  Zhuang Liu,et al.  PEGylated nanographene oxide for delivery of water-insoluble cancer drugs. , 2008, Journal of the American Chemical Society.

[16]  Filip Braet,et al.  Carbon nanomaterials in biosensors: should you use nanotubes or graphene? , 2010, Angewandte Chemie.

[17]  Seeram Ramakrishna,et al.  Graphene–Polymer Nanofiber Membrane for Ultrafast Photonics , 2010 .

[18]  L. Nicolais,et al.  Nanocomposites by melt intercalation based on polycaprolactone and organoclay , 2003 .

[19]  Geunhyung Kim,et al.  Fabrication of electrospun polycaprolactone biocomposites reinforced with chitosan for the proliferation of mesenchymal stem cells , 2011 .

[20]  J. Vacanti,et al.  A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. , 2003, Biomaterials.

[21]  S. Ramakrishna,et al.  Characterization of the surface biocompatibility of the electrospun PCL-collagen nanofibers using fibroblasts. , 2005, Biomacromolecules.

[22]  Edward J. Garboczi,et al.  A hybrid finite element-analytical method for determining the intrinsic elastic moduli of particles having moderately extended shapes and a wide range of elastic properties , 2006 .

[23]  S. Bhaduri,et al.  Rapid coating of Ti6Al4V at room temperature with a calcium phosphate solution similar to 10× simulated body fluid , 2004 .

[24]  P. Supaphol,et al.  Preparation and characterization of novel bone scaffolds based on electrospun polycaprolactone fibers filled with nanoparticles. , 2006, Macromolecular bioscience.

[25]  Yoshiki Ogawa,et al.  Tables of molecular vibrational frequencies , 1972 .

[26]  Y. Inoue,et al.  A new poly(l-lactide)-grafted graphite oxide composite: Facile synthesis, electrical properties and crystallization behaviors , 2010 .

[27]  Andreas Greiner,et al.  Electrospinning: a fascinating method for the preparation of ultrathin fibers. , 2007, Angewandte Chemie.

[28]  J. Kysar,et al.  Measurement of the Elastic Properties and Intrinsic Strength of Monolayer Graphene , 2008, Science.

[29]  Biqiong Chen,et al.  Synthesis and characterization of biomimetic hydroxyapatite/sepiolite nanocomposites. , 2011, Nanoscale.

[30]  Younan Xia,et al.  Coating electrospun poly(epsilon-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[31]  Hyoun‐Ee Kim,et al.  Nanostructured poly(epsilon-caprolactone)-silica xerogel fibrous membrane for guided bone regeneration. , 2010, Acta biomaterialia.