An Oxidase-Based Electrochemical Fluidic Sensor with High-Sensitivity and Low-Interference by On-Chip Oxygen Manipulation

Utilizing a simple fluidic structure, we demonstrate the improved performance of oxidase-based enzymatic biosensors. Electrolysis of water is utilized to generate bubbles to manipulate the oxygen microenvironment close to the biosensor in a fluidic channel. For the proper enzyme reactions to occur, a simple mechanical procedure of manipulating bubbles was developed to maximize the oxygen level while minimizing the pH change after electrolysis. The sensors show improved sensitivities based on the oxygen dependency of enzyme reaction. In addition, this oxygen-rich operation minimizes the ratio of electrochemical interference signal by ascorbic acid during sensor operation (i.e., amperometric detection of hydrogen peroxide). Although creatinine sensors have been used as the model system in this study, this method is applicable to many other biosensors that can use oxidase enzymes (e.g., glucose, alcohol, phenol, etc.) to implement a viable component for in-line fluidic sensor systems.

[1]  W. M. Haynes.  CRC Handbook of Chemistry and Physics , 1990 .

[2]  Jongwon Park,et al.  A simple on-chip self-diagnosis/self-calibration method of oxygen microsensor using electrochemically generated bubbles , 2005 .

[3]  Glucose enzyme electrode with percutaneous interface which operates independently of dissolved oxygen. , 1989, Clinical physics and physiological measurement : an official journal of the Hospital Physicists' Association, Deutsche Gesellschaft fur Medizinische Physik and the European Federation of Organisations for Medical Physics.

[4]  D. Gough,et al.  Development of the Implantable Glucose Sensor: What Are the Prospects and Why Is It Taking So Long? , 1995, Diabetes.

[5]  R. C. Weast.  CRC Handbook of Chemistry and Physics , 1973 .

[6]  J. Wang,et al.  Myoglobin-containing carbon-paste enzyme microelectrodes for the biosensing of glucose under oxygen-deficit conditions. , 1999, Analytical chemistry.

[7]  Joseph Wang,et al.  Oxygen-Rich Oxidase Enzyme Electrodes for Operation in Oxygen-Free Solutions , 1998 .

[8]  J. Lowry,et al.  Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose , 2002, Journal of Neuroscience Methods.

[9]  Sven-Olof Enfors,et al.  Oxygen-stabilized enzyme electrode for d-glucose analysis in fermentation broths , 1981 .

[10]  R. Buck,et al.  Miniaturized biosensors employing electropolymerized permselective films and their use for creatine assays in human serum. , 1996, Analytical chemistry.

[11]  Hiroaki Suzuki,et al.  Integrated microfluidic system with electrochemically actuated on-chip pumps and valves , 2003 .

[12]  A. Killard,et al.  Creatinine biosensors: principles and designs. , 2000, Trends in biotechnology.

[13]  Joseph Wang,et al.  Oxygen-independent poly(dimethylsiloxane)-based carbon-paste glucose biosensors. , 2002, Biosensors & bioelectronics.

[14]  P. Abel,et al.  Biosensors for in vivo glucose measurement: can we cross the experimental stage. , 2002, Biosensors & bioelectronics.

[15]  K. Yoda,et al.  Multi-enzyme membrane electrodes for determination of creatinine and creatine in serum. , 1983, Clinical chemistry.