Random transposon vectors pUTTns for the markerless integration of exogenous genes into gram-negative eubacteria chromosomes.

[1]  Jiandong Jiang,et al.  Isolation of an isocarbophos-degrading strain of Arthrobacter sp. scl-2 and identification of the degradation pathway. , 2009, Journal of microbiology and biotechnology.

[2]  Zhong‐Ji Qian,et al.  In vitro antioxidant activity of 5-HMF isolated from marine red alga Laurencia undulata in free-radical-mediated oxidative systems. , 2009, Journal of microbiology and biotechnology.

[3]  H. Schweizer,et al.  Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp. , 2009, FEMS microbiology letters.

[4]  I. Beacham,et al.  Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates. , 2009, Journal of microbiological methods.

[5]  Li Shun-peng Isolation, identification and characteristics of a dimethoate-degrading bacterium L3. , 2008 .

[6]  Yan Sun,et al.  Analysis of the role of LinA and LinB in biodegradation of delta-hexachlorocyclohexane. , 2007, Environmental microbiology.

[7]  J. Jiang,et al.  Parameters controlling the gene‐targeting frequency at the Sphingomonas species rrn site and expression of the methyl parathion hydrolase gene , 2007, Journal of applied microbiology.

[8]  W. Wackernagel,et al.  A double kill gene cassette for the positive selection of transforming non-selective DNA segments in Acinetobacter baylyi BD413. , 2007, Journal of microbiological methods.

[9]  K. Knoke,et al.  Use of Transposon Promoter-Probe Vectors in the Metabolic Engineering of the Obligate Methanotroph Methylomonas sp. Strain 16a for Enhanced C40 Carotenoid Synthesis , 2007, Applied and Environmental Microbiology.

[10]  Ruifu Zhang,et al.  Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon , 2007, Biodegradation.

[11]  D. Mazel,et al.  Construction of a Vibrio splendidus Mutant Lacking the Metalloprotease Gene vsm by Use of a Novel Counterselectable Suicide Vector , 2006, Applied and Environmental Microbiology.

[12]  M. Griffiths,et al.  Enhancement of Polyunsaturated Fatty Acid Production by Tn5 Transposon in Shewanella baltica , 2006, Biotechnology Letters.

[13]  Z. Cui,et al.  mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis , 2006, Nucleic acids research.

[14]  G. Dunny,et al.  Development of a Method for Markerless Genetic Exchange in Enterococcus faecalis and Its Use in Construction of a srtA Mutant , 2005, Applied and Environmental Microbiology.

[15]  Ruifu Zhang,et al.  Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes. , 2005, Canadian journal of microbiology.

[16]  B. Joris,et al.  New Integrative Method To Generate Bacillus subtilis Recombinant Strains Free of Selection Markers , 2004, Applied and Environmental Microbiology.

[17]  R. Roop,et al.  Intact Purine Biosynthesis Pathways Are Required for Wild-Type Virulence of Brucella abortus 2308 in the BALB/c Mouse Model , 2004, Infection and Immunity.

[18]  Kenneth N Timmis,et al.  Pseudomonas putida: a cosmopolitan opportunist par excellence. , 2002, Environmental microbiology.

[19]  P. Noirot,et al.  A new mutation delivery system for genome‐scale approaches in Bacillus subtilis , 2002, Molecular microbiology.

[20]  Shan-Chwen Chang,et al.  Cloning of a Cation Efflux Pump Gene Associated with Chlorhexidine Resistance in Klebsiella pneumoniae , 2002, Antimicrobial Agents and Chemotherapy.

[21]  Z. Cui,et al.  Isolation of Methyl Parathion-Degrading Strain M6 and Cloning of the Methyl Parathion Hydrolase Gene , 2001, Applied and Environmental Microbiology.

[22]  L. Furlaneto,et al.  Survival and conjugal transfer between Bacillus thuringiensis strains in aquatic environment , 2000 .

[23]  J. Michiels,et al.  Bi-functional gfp- and gusA-containing mini-Tn5 transposon derivatives for combined gene expression and bacterial localization studies. , 1999, Journal of microbiological methods.

[24]  K. Murata,et al.  alpha-L-rhamnosidase of Sphingomonas sp. R1 producing an unusual exopolysaccharide of sphingan. , 1998, Bioscience, biotechnology, and biochemistry.

[25]  G. Church,et al.  Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization , 1997, Journal of bacteriology.

[26]  G. Bennett,et al.  A method for construction of E. coli strains with multiple DNA insertions in the chromosome. , 1997, Gene.

[27]  B. Gicquel,et al.  Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria , 1996, Journal of bacteriology.

[28]  C. Bloch,et al.  New tools for integrated genetic and physical analyses of the Escherichia coli chromosome. , 1995, Gene.

[29]  R. Roop,et al.  pBBR1MCS: a broad-host-range cloning vector. , 1994, BioTechniques.

[30]  A. Pühler,et al.  Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans , 1992, Journal of bacteriology.

[31]  V. de Lorenzo,et al.  Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria , 1990, Journal of bacteriology.

[32]  V. de Lorenzo,et al.  Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria , 1990, Journal of bacteriology.

[33]  B. Washburn,et al.  New method for generating deletions and gene replacements in Escherichia coli , 1989, Journal of bacteriology.

[34]  N. Cianciotto,et al.  Site-specific mutagenesis in Legionella pneumophila by allelic exchange using counterselectable ColE1 vectors , 1988 .

[35]  A. Collmer,et al.  An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis. , 1987, Gene.

[36]  D. le Coq,et al.  Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria , 1985, Journal of bacteriology.

[37]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[38]  R. Dedonder [86] Levansucrase from Bacillus subtilis , 1966 .