Effect of Batch-Process Solar Disinfection on Survival of Cryptosporidium parvum Oocysts in Drinking Water

ABSTRACT The results of batch-process solar disinfection (SODIS) of Cryptosporidium parvum oocysts in water are reported. Oocyst suspensions were exposed to simulated sunlight (830 W m−2) at 40°C. Viability assays (4′,6′-diamidino-2-phenylindole [DAPI]/propidium iodide and excystation) and infectivity tests (Swiss CD-1 suckling mice) were performed. SODIS exposures of 6 and 12 h reduced oocyst infectivity from 100% to 7.5% (standard deviation = 2.3) and 0% (standard deviation = 0.0), respectively.

[1]  K. McGuigan,et al.  Effect of agitation, turbidity, aluminium foil reflectors and container volume on the inactivation efficiency of batch-process solar disinfectors. , 2001, Water research.

[2]  Conroy,et al.  Solar disinfection of drinking water contained in transparent plastic bottles : characterizing the bacterial inactivation process , 1998, Journal of applied microbiology.

[3]  S. Saito,et al.  Effects of ozonation and chlorination on viability and infectivity of Cryptosporidium parvum oocysts , 2000 .

[4]  R M Conroy,et al.  Inactivation of fecal bacteria in drinking water by solar heating , 1996, Applied and environmental microbiology.

[5]  M. Barer,et al.  Effects of simulated solar disinfection of water on infectivity of Salmonella typhimurium , 2000, Letters in applied microbiology.

[6]  M. Ares-Mazás,et al.  Effect of ultraviolet disinfection of drinking water on the viability of Cryptosporidium parvum oocysts. , 1993, The Journal of parasitology.

[7]  Kevin G McGuigan,et al.  Solar disinfection of drinking water and diarrhoea in Maasai children: a controlled field trial , 1996, The Lancet.

[8]  M. Barer,et al.  Batch process solar disinfection is an efficient means of disinfecting drinking water contaminated with Shigella dysenteriae type I , 2004, Letters in applied microbiology.

[9]  P. Dear,et al.  Detection and identification by real time PCR/RFLP analyses of Cryptosporidium species from human faeces , 2004, Letters in applied microbiology.

[10]  E. Ares-Mazás,et al.  Study of the combined influence of environmental factors on viability of cryptosporidium parvum oocysts in water evaluated by fluorogenic vital dyes and excystation techniques. , 2000, Veterinary parasitology.

[11]  R M Conroy,et al.  Solar disinfection of drinking water protects against cholera in children under 6 years of age , 2001, Archives of disease in childhood.

[12]  Peter Shanahan,et al.  Solar disinfection (SODIS): simulation of solar radiation for global assessment and application for point-of-use water treatment in Haiti. , 2003, Water research.

[13]  Martin Wegelin,et al.  SODIS-AN EMERGING WATER TREATMENT PROCESS , 1997 .

[14]  K. Oguma,et al.  Efficacy of UV Irradiation in Inactivating Cryptosporidiumparvum Oocysts , 2002, Applied and Environmental Microbiology.

[15]  R M Conroy,et al.  Solar disinfection: use of sunlight to decontaminate drinking water in developing countries. , 1999, Journal of medical microbiology.