Aggregating Brain Cell Cultures: A Model to Study Myelination and Demyelination

Cell cultures of the central nervous system (CNS) provide the investigator with a system to study selected factors influencing development without the complexity of in vivo studies. Rotation-mediated aggregating cell cultures of dissociated fetal brain follow the pattern of normal brain development (Matthieu et al., 1978; Seeds and Haffke, 1978; Trapp et al., 1979). Within a one month culture period, the aggregates develop from agglomerates of undifferentiated cells to highly organized populations of fully differentiated neurons, astrocytes and oligodendroglia (Matthieu et al., 1978; Trapp et al., 1979). In these cultures, myelinated axons and synapses are present (Seeds and Vatter, 1971; Matthieu et al., 1978; Trapp et al., 1979).

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