Simultaneous immunoassay for the detection of two lung cancer markers using functionalized SERS nanoprobes.

A quick and reproducible SERS-based immunoassay, using functionalized hollow gold nanospheres and magnetic beads, has been developed. Here, a simultaneous detection of dual cancer markers in blood serum has been achieved under a single excitation wavelength. The accuracy and sensitivity for clinical sera from five patients confirms their diagnostic feasibility.

[1]  L. Terry,et al.  Implications of spatial and temporal changes in concentration of pyruvate and glucose in onion (Allium cepa L.) bulbs during controlled atmosphere storage , 2009 .

[2]  Leif O. Brown,et al.  Self-assembly approach to multiplexed surface-enhanced Raman spectral-encoder beads. , 2009, Analytical chemistry.

[3]  Rostislav Bukasov,et al.  Highly tunable infrared extinction properties of gold nanocrescents. , 2007, Nano letters.

[4]  L. Tay,et al.  Nanoaggregate‐Embedded Beads as Novel Raman Labels for Biodetection , 2009 .

[5]  Reinhard Niessner,et al.  Microarrays for the screening of allergen-specific IgE in human serum. , 2003, Analytical chemistry.

[6]  Hongyuan Chen,et al.  Enzyme‐Linked Immunoassay of α‐1‐Fetoprotein in Serum by Differential Pulse Voltammetry , 1999 .

[7]  Patrick A Johnson,et al.  Surface-enhanced Raman scattering detection of DNA derived from the west nile virus genome using magnetic capture of Raman-active gold nanoparticles. , 2011, Analytical chemistry.

[8]  J. Kong,et al.  Immunoassays based on microelectrodes arrayed on a silicon chip for high throughput screening of liver fibrosis markers in human serum. , 2006, Biosensors & bioelectronics.

[9]  Optimization of the preparation of glass-coated, dye-tagged metal nanoparticles as SERS substrates. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[10]  Thomas Huser,et al.  Improving nanoprobes using surface-enhanced Raman scattering from 30-nm hollow gold particles. , 2006, Analytical chemistry.

[11]  Andrew A Berlin,et al.  Composite organic-inorganic nanoparticles (COINs) with chemically encoded optical signatures. , 2005, Nano letters.

[12]  Igor L. Medintz,et al.  Multiplexed toxin analysis using four colors of quantum dot fluororeagents. , 2004, Analytical chemistry.

[13]  Naomi J. Halas,et al.  Controlling the surface enhanced Raman effect via the nanoshell geometry , 2003 .

[14]  Luke P. Lee,et al.  Nanophotonic crescent moon structures with sharp edge for ultrasensitive biomolecular detection by local electromagnetic field enhancement effect. , 2005, Nano letters.

[15]  H. Bonkovsky,et al.  567 Serum alpha-fetoprotein (AFP) levels in patients with advanced hepatitis C-associated liver disease without hepatocellular carcinoma: Results from the HALT-C trial , 2003 .

[16]  Jürgen Durner,et al.  Clinical chemistry: challenges for analytical chemistry and the nanosciences from medicine. , 2009, Angewandte Chemie.

[17]  S. Joo.  Characterization of self‐assembled phenyl and benzyl isothiocyanate thin films on Au surfaces , 2006 .

[18]  Ching-Lung Lai,et al.  Serological markers of liver cancer. , 2005, Best practice & research. Clinical gastroenterology.

[19]  J. Choo,et al.  Highly sensitive immunoassay of lung cancer marker carcinoembryonic antigen using surface-enhanced Raman scattering of hollow gold nanospheres. , 2009, Analytical chemistry.

[20]  Guodong Liu,et al.  Electrochemical coding for multiplexed immunoassays of proteins. , 2004, Analytical chemistry.

[21]  Y. Mori,et al.  Colorimetric multiplexed immunoassay using specific aggregation of antigenic peptide-modified luminous nanoparticles. , 2006, Analytica chimica acta.

[22]  Serge Cosnier,et al.  Photoelectrochemical immunosensor for label-free detection and quantification of anti-cholera toxin antibody. , 2006, Journal of the American Chemical Society.

[23]  Reinhard Niessner,et al.  Automated microarray system for the simultaneous detection of antibiotics in milk. , 2004, Analytical chemistry.

[24]  Yukihiro Ozaki,et al.  Fluorescein isothiocyanate linked immunoabsorbent assay based on surface-enhanced resonance Raman scattering. , 2008, Analytical chemistry.

[25]  S. Nie,et al.  Quantum dot bioconjugates for ultrasensitive nonisotopic detection. , 1998, Science.

[26]  Sanyang Han,et al.  Magnetic separation and immunoassay of multi-antigen based on surface enhanced Raman spectroscopy. , 2011, Chemical communications.

[27]  Michael S. Wilson,et al.  Multiplex measurement of seven tumor markers using an electrochemical protein chip. , 2006, Analytical chemistry.

[28]  Im Joo Rhyu,et al.  Surface-enhanced Raman scattering imaging of HER2 cancer markers overexpressed in single MCF7 cells using antibody conjugated hollow gold nanospheres. , 2009, Biosensors & bioelectronics.

[29]  Shenghong Hu,et al.  Detection of multiple proteins on one spot by laser ablation inductively coupled plasma mass spectrometry and application to immuno- microarray with element-tagged antibodies. , 2007, Analytical chemistry.

[30]  Hui Chen,et al.  A one-step homogeneous immunoassay for cancer biomarker detection using gold nanoparticle probes coupled with dynamic light scattering. , 2008, Journal of the American Chemical Society.