Mycobacterium seoulense sp. nov., a slowly growing scotochromogenic species.

A previously undescribed, slowly growing, scotochromogenic mycobacterium was isolated from a patient with symptomatic pulmonary infection during hsp65 sequence-based identification of Korean clinical isolates. Phenetic characteristics of this strain were generally similar to those of Mycobacterium nebraskense and Mycobacterium scrofulaceum. However, some phenetic characteristics differentiated it from these two species. Its 16S rRNA gene sequences were unique and phylogenetic analysis based on 16S rRNA gene sequences placed the organism in the slowly growing Mycobacterium group close to M. nebraskense and M. scrofulaceum. Its unique mycolic acid profiles and the results of phylogenetic analysis based on two independent alternative chronometer molecules, hsp65 and rpoB, confirmed the taxonomic status of this strain as representing a novel species. These data support the conclusion that this strain represents a novel mycobacterial species, for which the name Mycobacterium seoulense sp. nov. is proposed. The type strain is strain 03-19(T) (=DSM 44998(T)=KCTC 19146(T)).

[1]  Sun-Hyun Kim,et al.  Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). , 2005, International journal of systematic and evolutionary microbiology.

[2]  Sudhir Kumar,et al.  MEGA2: molecular evolutionary genetics analysis software , 2001, Bioinform..

[3]  Yoon-Hoh Kook,et al.  Identification of Mycobacterial Species by Comparative Sequence Analysis of the RNA Polymerase Gene (rpoB) , 1999, Journal of Clinical Microbiology.

[4]  W. Ludwig,et al.  Differentiation of Phylogenetically Related Slowly Growing Mycobacteria Based on 16S-23S rRNA Gene Internal Transcribed Spacer Sequences , 1998, Journal of Clinical Microbiology.

[5]  J. T. Crawford,et al.  Characterization of an SAV organism and proposal of Mycobacterium triplex sp. nov , 1996, Journal of clinical microbiology.

[6]  L. Guthertz,et al.  Improved rapid identification of mycobacteria by combining solid-phase extraction with high-performance liquid chromatography analysis of BACTEC cultures , 1996, Journal of clinical microbiology.

[7]  E. Böttger,et al.  Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods , 1996, Journal of clinical microbiology.

[8]  W. Weisburg,et al.  Comparison of Mycobacterium 23S rRNA sequences by high-temperature reverse transcription and PCR. , 1995, International journal of systematic bacteriology.

[9]  E. Böttger,et al.  Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory , 1993, Journal of clinical microbiology.

[10]  J. Kilburn,et al.  Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography , 1992, Journal of clinical microbiology.

[11]  D. Stahl,et al.  The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria , 1990, Journal of bacteriology.

[12]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[13]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[14]  W. Fitch.  Toward Defining the Course of Evolution: Minimum Change for a Specific Tree Topology , 1971 .

[15]  M. Goodfellow,et al.  Taxonomy of Mycobacteria , 1998 .

[16]  T. Jukes.  CHAPTER 24 – Evolution of Protein Molecules , 1969 .