Pulsed electric field pretreatment of rapeseed green biomass (stems) to enhance pressing and extractives recovery.

The objective of this study was to investigate the effects of pulsed electric field (PEF) pretreatment on the valorization of extractives (proteins and polyphenols) from rapeseed green biomass (stems) by pressing. The effect of pressure, electric field strength and pulse number on the juice expression yield, total polyphenols and total proteins content in the expressed juices were studied. Experiments conducted under optimal conditions (E = 8 kV/cm, tPEF = 2 ms, P = 10 bar) permitted to increase the juice expressed yield from 34% to 81%. Significant increases in total polyphenols content (0.48 vs. 0.10 g GAE/100g DM), in total proteins content (0.14 vs. 0.07 g BSA/100g DM) and in consolidation coefficient (9.0 × 10(-8) vs. 2.2 × 10(-8)m(2)/s) were also observed after PEF pretreatment. The recovered press cake was well dehydrated with an increase of dry matter content from 8.8% to 53.0%.

[1]  E. Vorobiev,et al.  Impact of pulsed electric field and preheating on the lime purification of raw sugar beet expressed juices , 2015 .

[2]  E. Vorobiev,et al.  Selective extraction from microalgae Nannochloropsis sp. using different methods of cell disruption. , 2014, Bioresource technology.

[3]  K. Vishwanathan,et al.  Wet grinding characteristics of soybean for soymilk extraction. , 2011 .

[4]  Irini Angelidaki,et al.  Optimization of H2SO4-catalyzed hydrothermal pretreatment of rapeseed straw for bioconversion to ethanol: focusing on pretreatment at high solids content. , 2009, Bioresource technology.

[5]  Nikolai Lebovka,et al.  Pulsed-Electric-Fields-Induced Effects in Plant Tissues: Fundamental Aspects and Perspectives of Applications , 2009 .

[6]  M. Peleg CHARACTERIZATION OF THE STRESS RELAXATION CURVES OF SOLID FOODS , 1979 .

[7]  S. Nakatsuka,et al.  The Terzaghi-Voigt combined model for constant-pressure consolidation of filter cakes and homogeneous semi-solid materials , 1986 .

[8]  Gustavo V. Barbosa-Cánovas,et al.  Fundamentals of High-Intensity Pulsed Electric Fields (PEF) , 1999 .

[9]  Günther Laufenberg,et al.  Transformation of vegetable waste into value added products: (A) the upgrading concept; (B) practical implementations. , 2003, Bioresource technology.

[10]  Eugène Vorobiev,et al.  Solid–liquid expression from denaturated plant tissue: Filtration–consolidation behaviour , 2010 .

[11]  F. Chemat,et al.  "Solvent-free" ultrasound-assisted extraction of lipids from fresh microalgae cells: a green, clean and scalable process. , 2012, Bioresource technology.

[12]  J. Vaxelaire,et al.  Impact of apple processing modes on extracted juice quality: Pressing assisted by pulsed electric fields , 2011 .

[13]  K. Rosentrater,et al.  Effect of co-products of enzyme-assisted aqueous extraction of soybeans on ethanol production in dry-grind corn fermentation. , 2015, Bioresource technology.

[14]  T. Beveridge Juice extraction from apples and other fruits and vegetables. , 1997, Critical reviews in food science and nutrition.

[15]  E. Vorobiev,et al.  Filtration diffusivity and expression behaviour of thermally and electrically pretreated sugar beet tissue and press-cake , 2012 .

[16]  Ignacio Ballesteros,et al.  Optimization of uncatalyzed steam explosion pretreatment of rapeseed straw for biofuel production. , 2015, Bioresource technology.

[17]  R. Boydston,et al.  Rapeseed (Brassica napus) Green Manure Crop Suppresses Weeds in Potato (Solanum tuberosum) , 1995, Weed Technology.

[18]  Masahiro Saito,et al.  SELECTIVE RELEASE OF INTRACELLULAR PROTEIN USING PULSED ELECTRIC FIELD , 1995 .

[19]  H. Sixta,et al.  Potential of hot water extraction of birch wood to produce high-purity dissolving pulp after alkaline pulping. , 2013, Bioresource technology.

[20]  M. Farag,et al.  Metabolome classification of Brassica napus L. organs via UPLC-QTOF-PDA-MS and their anti-oxidant potential. , 2013, Phytochemical analysis : PCA.

[21]  N. Jain,et al.  Emission of Air Pollutants from Crop Residue Burning in India , 2014 .

[22]  N. López,et al.  Evaluation of phenolic extraction during fermentation of red grapes treated by a continuous pulsed electric fields process at pilot-plant scale , 2010 .

[23]  Ahmad Jahan Latibari,et al.  Fiber Morphology and Chemical Composition of Rapeseed (Brassica Napus) Stems , 2010 .

[24]  E. Vorobiev,et al.  A new way for the oil plant biomass valorization: Polyphenols and proteins extraction from rapeseed stems and leaves assisted by pulsed electric fields , 2015 .

[25]  L. Šuklje,et al.  Rheologic aspects of soil mechanics , 1969 .

[26]  E. Vorobiev,et al.  Intensification of polyphenols extraction from grape seeds by high voltage electrical discharges and extract concentration by dead-end ultrafiltration , 2011 .

[27]  Eugène Vorobiev,et al.  Pulsed electric field and pH assisted selective extraction of intracellular components from microalgae Nannochloropsis , 2015 .

[28]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[29]  Enhanced Extraction from Solid Foods and Biosuspensions by Pulsed Electrical Energy , 2010 .

[30]  H. Kim,et al.  Effect of freezing pretreatment on the quality of juice extracted from Prunus mume fruit by osmosis with sucrose , 2013 .