A palmtop-sized rotary-drive-type biochemical analysis system by magnetic bead handling

By applying a single rotary motion to our previously developed magnetic bead droplet handling mechanism, we recently developed transportation and agitation operation mechanisms aiming at realizing a portable-sized biochemical analysis system. We used two permanent magnets for transporting beads and another magnet coupled with multi-layered flat coils for agitation. They are all mounted on a rotational table, and the total size of the system is 60 × 45 × 35 mm3. We experimentally confirmed that the developed system can do all the bead handlings (transportation, extraction, fusion and agitation) by rotational motion only.

[1]  Jens Anders Branebjerg,et al.  Microfluidics-a review , 1993 .

[2]  N. Nguyen,et al.  Fundamentals and Applications of Microfluidics , 2002 .

[3]  U. Lehmann,et al.  Two dimensional magnetic manipulation of microdroplets on a chip , 2005, The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05..

[4]  D. Liepmann,et al.  A PLANAR MICROPUMP UTILIZING THERMOPNEUMATIC ACTUATION , 2004 .

[5]  R. Austin,et al.  Design of a microfabricated magnetic cell separator , 2001, Electrophoresis.

[6]  N. Kasagi,et al.  A chaotic mixer for magnetic bead-based micro cell sorter , 2004, Journal of Microelectromechanical Systems.

[7]  大房 健 基礎講座 電気泳動(Electrophoresis) , 2005 .

[8]  S. Cho,et al.  Towards digital microfluidic circuits: creating, transporting, cutting and merging liquid droplets by electrowetting-based actuation , 2002, Technical Digest. MEMS 2002 IEEE International Conference. Fifteenth IEEE International Conference on Micro Electro Mechanical Systems (Cat. No.02CH37266).

[9]  Mitsuhiro Shikida,et al.  Using wettability and interfacial tension to handle droplets of magnetic beads in a micro-chemical-analysis system , 2006 .

[10]  Andreas Manz,et al.  On-chip free-flow magnetophoresis: continuous flow separation of magnetic particles and agglomerates. , 2004, Analytical chemistry.

[11]  Jeung Sang Go,et al.  A disposable, dead volume-free and leak-free monolithic PDMS microvalve , 2003, TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664).

[12]  Hiroshi Ito,et al.  Development of an enzymatic reaction device using magnetic bead-cluster handling , 2006 .

[13]  Nam-Trung Nguyen,et al.  Micromixers?a review , 2005 .

[14]  M. Gijs,et al.  Plastic micropump with ferrofluidic actuation , 2005, Journal of Microelectromechanical Systems.

[15]  Chang-Jin Kim,et al.  Particle separation and concentration control for digital microfluidic systems , 2003, The Sixteenth Annual International Conference on Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE.

[16]  C. Kim,et al.  Enhancement of mixing by droplet-based microfluidics , 2002, Technical Digest. MEMS 2002 IEEE International Conference. Fifteenth IEEE International Conference on Micro Electro Mechanical Systems (Cat. No.02CH37266).

[17]  Tero Soukka,et al.  Droplet Manipulation on a Superhydrophobic Surface for Microchemical Analysis , 2001 .

[18]  D. Beebe,et al.  A valved responsive hydrogel microdispensing device with integrated pressure source , 2004, Journal of Microelectromechanical Systems.

[19]  Masayoshi Esashi,et al.  Microflow devices and systems , 1994 .

[20]  Jaephil Do,et al.  A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities. , 2008, Lab on a chip.

[21]  A. Helmicki,et al.  An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities. , 2002, Lab on a chip.

[22]  Martin A. M. Gijs,et al.  Two dimensional magnetic manipulation of microdroplets on a chip , 2005 .

[23]  Shih-Kang Fan,et al.  Manipulation of multiple droplets on N/spl times/M grid by cross-reference EWOD driving scheme and pressure-contact packaging , 2003, The Sixteenth Annual International Conference on Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE.

[24]  Manish Biyani,et al.  High-throughput screening of mutant AKR enzymes using mRNA display and novel microreactor array chips , 2005 .

[25]  Jeung Sang Go,et al.  A novel fabrication of in-channel 3-D micromesh structure using maskless multi-angle exposure and its microfilter application , 2003, The Sixteenth Annual International Conference on Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE.

[26]  T. Ichiki,et al.  Topographic and chemical control of cells' configuration on micro-patterned scaffold chips with aligned beads , 2005 .

[27]  A. Rida,et al.  Planar coil-based microsystem for the long-range transport of magnetic beads , 2003, TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664).