Stability and Reliability of Plasma Level of Lipid Biomarkers and Their Correlation with Dietary Fat Intake

The reliability and stability of plasma lipid biomarkers and their association with dietary fat intake were evaluated among 48 subjects who were randomly chosen from the participants of a validation study of the population-based cohort, the Shanghai Men's Health Study (SMHS). Four spot blood samples, one taken each season, were measured for total cholesterol, triglyceride, HDL-cholesterol, and LDL-cholesterol levels. The reliability and stability of these measurements were assessed by intraclass correlation coefficients (ICC) and by the correlations between a randomly chosen measurement with the mean of measurements across seasons using a bootstrap approach. The median levels for total cholesterol, triglycerides, HDL-cholesterol, and LDL-cholesterol were 177.5, 164.5, 41.0, and 102.5 (mg/dl), respectively. The ICCs of the biomarkers ranged from 0.58 (LDL-cholesterol) to 0.83 (HDL-cholesterol). The correlation between randomly chosen spot measurements and the mean measurement were 0.91, 0.86, 0.93, and 0.83 for total cholesterol, triglycerides, HDL-cholesterol, and LDL-cholesterol, respectively. The correlations of lipid biomarkers with dietary fat intake and other lifestyle factors were comparable to other previous reports. In conclusion, this study suggests that measurements of lipid biomarkers from a single spot blood sample are a good representation of the average blood levels of these biomarkers in the study population and could be a useful tool for epidemiological studies.

[1]  Jing Chen,et al.  Prevalence of the metabolic syndrome and overweight among adults in China , 2005, The Lancet.

[2]  Jing Chen,et al.  Prevalence, Awareness, Treatment, and Control of Hypertension in China , 2002, Hypertension.

[3]  C. Yajnik The lifecycle effects of nutrition and body size on adult adiposity, diabetes and cardiovascular disease , 2002, Obesity reviews : an official journal of the International Association for the Study of Obesity.

[4]  S. Kasim-Karakas,et al.  Changes in plasma lipoproteins during low-fat, high-carbohydrate diets: effects of energy intake. , 2000, The American journal of clinical nutrition.

[5]  R. Krauss,et al.  Effects of a low-fat, high-carbohydrate diet on VLDL-triglyceride assembly, production, and clearance. , 1999, The Journal of clinical investigation.

[6]  J. Hokanson,et al.  Hypertriglyceridemia as a cardiovascular risk factor. , 1998, The American journal of cardiology.

[7]  Xi-gui Wu,et al.  Prevalence of hypertension and its trends in Chinese populations. , 1995, International journal of cardiology.

[8]  J. Manson,et al.  Reproducibility of plasma hormone levels in postmenopausal women over a 2-3-year period. , 1995, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[9]  R. Havel,et al.  Effects of dietary cholesterol and fat saturation on plasma lipoproteins in an ethnically diverse population of healthy young men. , 1995, The Journal of clinical investigation.

[10]  B Rosner,et al.  Correction of logistic regression relative risk estimates and confidence intervals for random within-person measurement error. , 1992, American journal of epidemiology.

[11]  S. Connor,et al.  Will a high-carbohydrate, low-fat diet lower plasma lipids and lipoproteins without producing hypertriglyceridemia? , 1991, Arteriosclerosis and thrombosis : a journal of vascular biology.

[12]  J. Dietschy,et al.  Interaction of dietary cholesterol and triglycerides in the regulation of hepatic low density lipoprotein transport in the hamster. , 1988, The Journal of clinical investigation.

[13]  P. Wilson,et al.  Incidence of coronary heart disease and lipoprotein cholesterol levels. The Framingham Study. , 1986, JAMA.

[14]  R. Levy,et al.  Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. , 1972, Clinical chemistry.

[15]  C. Dolea,et al.  World Health Organization , 1949, International Organization.

[16]  A. Brantsaeter,et al.  The serum LDL/HDL cholesterol ratio is influenced more favorably by exchanging saturated with unsaturated fat than by reducing saturated fat in the diet of women. , 2003, The Journal of nutrition.

[17]  D. Russell,et al.  Blood cholesterol screening influence of fasting state on cholesterol results and management decisions. , 2000, Journal of general internal medicine.

[18]  D. Hunter Methodological issues in the use of biological markers in cancer epidemiology: cohort studies. , 1997, IARC scientific publications.

[19]  Hunter Dj Methodological issues in the use of biological markers in cancer epidemiology: cohort studies. , 1997 .

[20]  J. Murray,et al.  The Global Burden of Disease , 1996 .

[21]  K. McGraw,et al.  Forming inferences about some intraclass correlation coefficients. , 1996 .

[22]  D. Gordon,et al.  High-density lipoprotein cholesterol and cardiovascular disease. Four prospective American studies. , 1989, Circulation.

[23]  The Lipid Research Clinics Coronary Primary Prevention Trial results. II. The relationship of reduction in incidence of coronary heart disease to cholesterol lowering. , 1984, JAMA.