LOCALIZATION OF STRUCTURAL POLYMERS IN THE CELL WALL OF NEUROSPORA CRASSA

The distribution and localization of structural polymers in the cell wall of Neurospora crassa has been studied by selective removal and light and electron microscope examination. Observations with the light microscope indicated that each polymer by itself can provide structural integrity to the cell wall. Examination by electron microscopy showed that the cell wall consists of an outer layer of thick fibrils, identified chemically as a glucan-peptide-galactosamine complex, and an inner layer made up of β-1,3 glucan and thin fibrils of chitin.

[1]  L. Hawker.  FINE STRUCTURE OF FUNGI AS REVEALED BY ELECTRON MICROSCOPY , 1965, Biological reviews of the Cambridge Philosophical Society.

[2]  K. Horikoshi,et al.  STUDIES OF THE SPORE COATS OF FUNGI. I. ISOLATION AND COMPOSITION OF THE SPORE COATS OF ASPERGILLUS ORYZAE. , 1964, Biochimica et biophysica acta.

[3]  M. Alexander,et al.  Dissolution of fungal cell walls by a streptomycete chitinase and beta-(1-3) glucanase. , 1965, Archives of biochemistry and biophysics.

[4]  E. Kellenberger,et al.  Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. , 1958 .

[5]  John H. Luft,et al.  IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS , 1961, The Journal of biophysical and biochemical cytology.

[6]  D. Northcote,et al.  The chemical composition and structure of the yeast cell wall. , 1952, The Biochemical journal.

[7]  W. E. Trevelyan,et al.  Studies on yeast metabolism. 1. Fractionation and microdetermination of cell carbohydrates , 1952 .

[8]  E. Tatum,et al.  A RELATIONSHIP BETWEEN CELL WALL STRUCTURE AND COLONIAL GROWTH IN NEUROSPORA CRASSA , 1963 .

[9]  P. Mahadevan,et al.  Relationship of the Major Constituents of the Neurospora crassa Cell Wall to Wild-Type and Colonial Morphology , 1965, Journal of bacteriology.

[10]  A. Gornall,et al.  Determination of serum proteins by means of the biuret reaction. , 1949, The Journal of biological chemistry.

[11]  H. Ludwig.  Chitin , 2022 .