Structural characterization of water-insoluble nonstarchy polysaccharides of oats and barley

Abstract Water-insoluble dietary fibre of dehulled oat and barley grain was isolated with enzymatic hydrolysis of starch and protein and further extracted sequentially. The extractants used were saturated barium hydroxide, water, 1 M potassium hydroxide and 4 M sodium hydroxide. The fractions were dialysed against pure water, freeze-dried and weighed. Repeatable recoveries of extracts were obtained. The ground grains and extracted fractions were characterized for their fat and protein content. The fractions were hydrolysed and characterized by measuring their neutral sugar composition using gas chromatography. Comparison of the amounts of cellulosic and noncellulosic polysaccharides was made. Further characterization of fractions was performed with solid-state 13 C CP/MAS NMR spectroscopy and IR spectroscopy. The differences in composition of insoluble material between oats and barley were small. The distribution of masses and monosaccharides of the five fractions obtained were similar and the measured spectra show the same characteristic signals as saccharide structures.

[1]  B. Lewis,et al.  Enzymic determination of β-glucan in cereal-based food products , 1990 .

[2]  D. Jacobs,et al.  Consumption of whole-grain foods and decreased risk of cancer : Proposed mechanisms , 2000 .

[3]  R. Codd,et al.  Measurement of (1 → 3),(1 → 4)‐β‐D‐glucan in barley and oats: A streamlined enzymic procedure , 1991 .

[4]  S. Maunu,et al.  Structural characterization of water soluble β-glucan of oat bran. , 2000 .

[5]  N. Asp,et al.  Rapid enzymatic assay of insoluble and soluble dietary fiber. , 1983, Journal of agricultural and food chemistry.

[6]  J. Delcour,et al.  Physicochemical and Functional Properties of Rye Nonstarch Polysaccharides. VI. Variability in the Structure of Water-Unextractable Arabinoxylans , 1995 .

[7]  A. Ebringerová,et al.  Infrared study of arabinoxylans , 1994 .

[8]  V. Sasinková,et al.  Influence of the drying method on the physical properties and immunomodulatory activity of the particulate (1→3)-β-d-glucan from Saccharomyces cerevisiae , 2003 .

[9]  M. JonesJulie,et al.  The definition of dietary fibers , 2001 .

[10]  R. Marchessault,et al.  High-resolution carbon-13 CP/MAS NMR spectra of solid cellulose oligomers and the structure of cellulose II , 1983 .

[11]  B. Blackwell,et al.  Structural studies of (1→3),(1→4)-β-D-glucans by 13C-nuclear magnetic resonance spectroscopy and by rapid analysis of cellulose-like regions using high-performance anion-exchange chromatography of oligosaccharides released by lichenase , 1994 .

[12]  R. Hamer,et al.  Barium hydroxide as a tool to extract pure arabinoxylans from water-insoluble cell wall material of wheat flour , 1991 .

[13]  P. Åman,et al.  Isolation and chemical characterization of water-soluble mixed-linked β-glucans and arabinoxylans in oat milling fractions , 1993 .

[14]  P. Wood,et al.  Evaluation of role of concentration and molecular weight of oat β-glucan in determining effect of viscosity on plasma glucose and insulin following an oral glucose load , 2000, British Journal of Nutrition.

[15]  C. Weber,et al.  Extraction and fractionation of insoluble fiber from five fiber sources , 1996 .

[16]  M. Davies,et al.  A 13C CP/MAS NMR spectroscopy and AFM study of the structure of Glucagel™, a gelling β-glucan from barley , 1999 .

[17]  P. Åman,et al.  Structure and components of dietary fiber , 1993 .

[18]  Jan A. Delcour,et al.  Alkaline Hydrogen Peroxide Extraction of Wheat Bran Non-starch Polysaccharides , 2001 .

[19]  J. Anderson,et al.  Ten different dietary fibers have significantly different effects on serum and liver lipids of cholesterol-fed rats. , 1994, The Journal of nutrition.

[20]  J. Duus,et al.  Assignment of structures to oligosaccharides produced by enzymic degradation of a beta-D-glucan from barley by 1H- and 13C-n.m.r. spectroscopy. , 1991, Carbohydrate research.

[21]  M. Izydorczyk,et al.  Structure and physicochemical properties of barley non-starch polysaccharides — I. Water-extractable β-glucans and arabinoxylans☆ , 1998 .

[22]  P. Ekholm,et al.  Effects of Natural Chelating Agents on the Solubility of Some Physiologically Important Mineral Elements in Oat Bran and Oat Flakes , 2000 .

[23]  M. Kačuráková,et al.  Developments in Mid-Infrared FT-IR Spectroscopy of Selected Carbohydrates , 2001 .

[24]  C. Fanelli,et al.  Solid-state (13)C CP MAS NMR spectroscopy of mushrooms gives directly the ratio between proteins and polysaccharides. , 2000, Journal of agricultural and food chemistry.

[25]  K. Poutanen,et al.  Development of a germination process for producing high β-glucan, whole grain food ingredients from oat , 2001 .

[26]  R. Henry,et al.  A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS , 1983 .

[27]  Czesława Paluszkiewicz,et al.  FT-IR and FT-Raman study of hydrothermally degradated cellulose , 2001 .

[28]  R. Furneaux,et al.  13C CP/MAS NMR study of the interaction of bile acids with barley β-d-glucan , 1996 .

[29]  Nikolaus Wellner,et al.  FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicelluloses , 2000 .

[30]  F. Manthey,et al.  Soluble and Insoluble Dietary Fiber Content and Composition in Oat , 1999 .

[31]  S. Kalra,et al.  Effect of Dietary Barley β-Glucan on Cholesterol and Lipoprotein Fractions in Rat , 2000 .

[32]  P. Ekholm,et al.  The effect of phytic acid and some natural chelating agents on the solubility of mineral elements in oat bran , 2003 .

[33]  P. Wood,et al.  Molecular characterization of cereal beta-glucans. II. Size-exclusion chromatography for camparison of molecular weight , 1991 .

[34]  J. Huvenne,et al.  Investigation of the glycosidic linkages in several oligosaccharides using FT-IR and FT Raman spectroscopies , 1995 .

[35]  P. Åman,et al.  Water unextractable polysaccharides from three milling fractions of rye grain , 1996 .

[36]  P. Manzanares,et al.  Physical and structural properties of barley (1 → 3),(1 →4)-β-d-glucan. Part I. Determination of molecular weight and macromolecular radius by light scattering , 1997 .

[37]  A. Gutiérrez,et al.  Structural characterization of extracellular polysaccharides produced by fungi from the genus Pleurotus. , 1996, Carbohydrate research.

[38]  T. S. Kahlon,et al.  Cholesterol-lowering effects in hamsters of β-glucan-enriched barley fraction, dehulled whole barley, rice bran, and oatbran and their combinations , 1993 .

[39]  A. Perlin,et al.  High-field, 13C-N.M.R. spectroscopy of β-d-glucans, amylopectin, and glycogen , 1982 .

[40]  R. Newman,et al.  Molecular ordering of cellulose after extraction of polysaccharides from primary cell walls of Arabidopsis thaliana: a solid-state CP/MAS (13)C NMR study. , 2002, Carbohydrate research.

[41]  R. Pettersen.  Wood Sugar Analysis by Anion Chromatography , 1991 .

[42]  P. Åman,et al.  Structural features of (1→3),(1→4)-β-d-glucan and arabinoxylan fractions isolated from rye bran , 2000 .

[43]  R. Hamer,et al.  Water-unextractable cell wall material from wheat flour. 1. Extraction of polymers with alkali. , 1992 .

[44]  N. Asp,et al.  Determination of insoluble, soluble, and total dietary fiber in foods and food products: interlaboratory study. , 1988, Journal - Association of Official Analytical Chemists.

[45]  P. Wood,et al.  Oat beta-glucan reduces blood cholesterol concentration in hypercholesterolemic subjects. , 1994, European journal of clinical nutrition.

[46]  Michael J. Gidley,et al.  High-resolution solid-state NMR of food materials , 1992 .

[47]  P. Åman,et al.  Isolation and chemical characterization of water soluble arabinoxylans in rye grain. , 1990 .

[48]  P. Åman,et al.  Chemical composition of some different types of barley grown in Montana, U.S.A , 1986 .