Combined natural antimicrobial treatments (EDTA, lysozyme, rosemary and oregano oil) on semi cooked coated chicken meat stored in vacuum packages at 4 °C: Microbiological and sensory evaluation

Abstract The present study examined the effect of natural antimicrobials: Ethylenediaminetetraacetate (EDTA), lysozyme, rosemary and oregano oil and their combinations, on the shelf-life of semi cooked coated chicken fillets stored under vacuum packaging (VP), at 4 ± 0.5 °C for a period of 18 days. The treatments of semi cooked coated chicken fillets examined in the present study were the following: Air-packaged (A, control samples), vacuum-packaged (VP), VP with EDTA–lysozyme solution 1.50% w/w, (VP + EL), VP with rosemary oil 0.20% v/w, (VP + R), VP with oregano oil 0.20% v/w, (VP + O), VP with EDTA–lysozyme solution and rosemary oil (VP + EL + R) and finally VP with EDTA–lysozyme and oregano oil (VP + EL + O). The shelf-life of the samples was determined using both microbiological and sensory analyses. Among the antimicrobial combinations examined in the present study, the treatments VP + EL + R and VP + EL + O were the most effective against the growth of Gram-negative, Gram-positive bacteria, and to a lesser extent on yeasts. Based on both microbiological (TVC data) and sensory (taste attribute) analyses, treatments: VP and VP + O gave a shelf life extension of 6 days, whereas treatments VP + EL + R and VP + EL + O produced a shelf-life extension of 7–8 days, as compared to the control samples. Industrial Relevance The present research has highlighted the use of natural antimicrobial treatment combinations, including: EDTA, lysozyme, rosemary and oregano oil and their combinations, in the extension of shelf-life of semi cooked coated chicken fillets stored under vacuum packaging at 4 °C for a period of 18 days. Establishing, the determination of the shelf-life of fresh poultry and products represents a challenge for food companies as poultry meat has a short shelf-life, which causes substantial practical problems for its distribution. Therefore, knowledge of natural preservatives, that can be used as alternatives to chemical additives, that could extend the products' shelf life can have an important economic feedback by reducing losses attributed to spoilage and by allowing the products to reach distant and new markets. This study has shown that combinations of natural antimicrobials can extend the shelf-life of the product.

[1]  M. Kontominas,et al.  Combined effect of freeze chilling and MAP on quality parameters of raw chicken fillets. , 2008, Food microbiology.

[2]  A. Mustapha,et al.  Reduction of Brochothrix thermosphacta and Salmonella Serotype Typhimurium on Vacuum-Packaged Fresh Beef Treated with Nisin and Nisin Combined with EDTA , 2002 .

[3]  J. Josephsen,et al.  The antimicrobial activity of acidocin CH5 in MRS broth and milk with added NaCl, NaNO3 and lysozyme. , 1998, International journal of food microbiology.

[4]  D. Ahn,et al.  Double-packaging is effective in reducing lipid oxidation and off-odor volatiles of irradiated raw turkey meat. , 2003, Poultry science.

[5]  P. Dawson,et al.  Incorporation of food-grade antimicrobial compounds into biodegradable packaging films. , 1998, Journal of food protection.

[6]  S. Brul,et al.  Preservative agents in foods. Mode of action and microbial resistance mechanisms. , 1999, International journal of food microbiology.

[7]  L. Beuchat,et al.  Presence and changes in populations of yeasts on raw and processed poultry products stored at refrigeration temperature. , 2000, International journal of food microbiology.

[8]  A D Russell,et al.  Mechanisms of bacterial resistance to non-antibiotics: food additives and food and pharmaceutical preservatives. , 1991, The Journal of applied bacteriology.

[9]  R. Holley,et al.  Antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms. , 1997, International journal of food microbiology.

[10]  Mark A. Harrison,et al.  Vacuum or Modified Atmosphere Packaging and EDTA-Nisin Treatment to Increase Poultry Product Shelf Life , 1999 .

[11]  S. Yurdugul,et al.  The effects of nisin, oil–wax coating and yogurt on the quality of refrigerated chicken meat , 2004 .

[12]  E. Smid,et al.  Influence of carvacrol on growth and toxin production by Bacillus cereus. , 2001, International journal of food microbiology.

[13]  A. Al-Shalhat,et al.  Microbiological and shelf life assessment of chilled eviscerated whole chicken broilers in Saudi Arabia. , 1994, British poultry science.

[14]  K. Sallam,et al.  Effects of Trisodium Phosphate and Sodium Chloride Dipping on the Microbial Quality and Shelf Life of Refrigerated Tray-packaged Chicken Breasts. , 2004, Food science and biotechnology.

[15]  C. Genigeorgis,et al.  Antimicrobial Effects of Sodium Tripolyphosphate Against Bacteria Attached to the Surface of Chicken Carcasses , 1997 .

[16]  P. Davidson,et al.  Enhancement of nisin, lysozyme, and monolaurin antimicrobial activities by ethylenediaminetetraacetic acid and lactoferrin. , 2004, International journal of food microbiology.

[17]  M. Salsi,et al.  Spoilage microflora in fresh chicken breast stored at 4 °C : influence of packaging methods , 1997 .

[18]  C. O. Gill,et al.  The control of microbial spoilage in fresh meats , 1986 .

[19]  K. Miyashita,et al.  Bacterial microflora of carp (Cyprinus carpio) and its shelf-life extension by essential oil compounds , 2004 .

[20]  Ioannis N. Savvaidis,et al.  Nisin–EDTA treatments and modified atmosphere packaging to increase fresh chicken meat shelf-life , 2009 .

[21]  G. Nychas,et al.  Storage of poultry meat under modified atmospheres or vacuum packs: possible role of microbial metabolites as indicator of spoilage. , 1994, The Journal of applied bacteriology.

[22]  I. Recio,et al.  Antibacterial and binding characteristics of bovine, ovine and caprine lactoferrins : a comparative study , 2000 .

[23]  D. L. Marshall,et al.  Microbiological, colour and sensory changes of refrigerated chicken legs treated with selected phosphates , 1999 .

[24]  Panagiotis N Skandamis,et al.  Preservation of fresh meat with active and modified atmosphere packaging conditions. , 2002, International journal of food microbiology.

[25]  J. McLay,et al.  Use of a ground beef model to assess the effect of the lactoperoxidase system on the growth of Escherichia coli O157:H7, Listeria monocytogenes and Staphylococcus aureus in red meat. , 2000, International journal of food microbiology.

[26]  M. Kontominas,et al.  Shelf-life of a chilled precooked chicken product stored in air and under modified atmospheres: microbiological, chemical, sensory attributes. , 2006, Food microbiology.

[27]  M. Stecchini,et al.  Effect of Essential Oils on Aeromonas hydrophila in a Culture Medium and in Cooked Pork. , 1993, Journal of food protection.

[28]  P. Aureli,et al.  Antimicrobial Activity of Some Plant Essential Oils Against Listeria monocytogenes 1. , 1992, Journal of food protection.

[29]  R. Holley,et al.  Inhibition of bacterial growth on ham and bologna by lysozyme, nisin and EDTA , 2000 .

[30]  D. Morrison,et al.  Lipopolysaccharide interaction with lysozyme. Binding of lipopolysaccharide to lysozyme and inhibition of lysozyme enzymatic activity. , 1989, The Journal of biological chemistry.

[31]  S. Burt,et al.  Essential oils: their antibacterial properties and potential applications in foods--a review. , 2004, International journal of food microbiology.

[32]  G. Nychas,et al.  Effect of oregano essential oil on microbiological and physico‐chemical attributes of minced meat stored in air and modified atmospheres , 2001, Journal of applied microbiology.

[33]  F. Nattress,et al.  Effects of treatment with lysozyme and nisin on the microflora and sensory properties of commercial pork. , 2003, International journal of food microbiology.

[34]  L. Gallo,et al.  Microbial spoilage of refrigerated fresh broilers. I. Bacterial flora and growth during storage , 1988 .

[35]  J. Delves-Broughton.  The use of EDTA to enhance the efficacy of nisin towards Gram-negative bacteria , 1993 .

[36]  L. Beuchat,et al.  Efficacy of Selected Chemicals for Killing Pathogenic and Spoilage Microorganisms on Chicken Skin. , 1995, Journal of food protection.

[37]  R. Repaske.  Lysis of gram-negative bacteria by lysozyme. , 1956, Biochimica et biophysica acta.

[38]  D. Mossel,et al.  Significance of Enterobacteriaceae as index organisms for hygiene on fresh untreated poultry, poultry treated with lactic acid and poultry stored in a modified atmosphere , 1994 .

[39]  B M Mackey,et al.  The relationship between the phenotypic properties of bacteria from chill-stored meat and spoilage processes. , 1992, Society for Applied Bacteriology symposium series.

[40]  R. Capita,et al.  Effect of various chemical decontamination treatments on natural microflora and sensory characteristics of poultry. , 2007, International journal of food microbiology.

[41]  M. Kontominas,et al.  Combined effect of oregano essential oil and modified atmosphere packaging on shelf-life extension of fresh chicken breast meat, stored at 4 °C , 2007 .

[42]  A. Yemenicioğlu,et al.  Antimicrobial and antioxidant activity of edible zein films incorporated with lysozyme, albumin proteins and disodium EDTA , 2007 .

[43]  J. Labadie.  Consequences of packaging on bacterial growth. Meat is an ecological niche. , 1999, Meat science.

[44]  J. W. Arnold,et al.  APC values and volatile compounds formed in commercially processed, raw chicken parts during storage at 4 and 13 °C and under simulated temperature abuse conditions† , 2000 .

[45]  J. G. Banks,et al.  Some factors influencing the recovery of yeasts and moulds from chilled foods , 1987 .

[46]  M. Kontominas,et al.  Potential of oregano essential oil and MAP to extend the shelf life of fresh swordfish: a comparative study with ice storage. , 2008, Journal of food science.

[47]  L. A. Roth,et al.  Studies on the bacterial flora of vacuum-packaged fresh beef. , 1972, Canadian journal of microbiology.

[48]  T. Chen,et al.  Microbiological Quality of Refrigerated Chicken Gizzards from Different Sources as Related to Their Shelf-Lives , 1979 .

[49]  P. Skandamis,et al.  Behaviour of Listeria monocytogenes and autochthonous flora on meat stored under aerobic, vacuum and modified atmosphere packaging conditions with or without the presence of oregano essential oil at 5 °C , 2000, Journal of applied microbiology.

[50]  M. Kontominas,et al.  Combined effect of irradiation and modified atmosphere packaging on shelf-life extension of chicken breast meat: microbiological, chemical and sensory changes , 2008 .

[51]  C. Vanderzant,et al.  Compendium of Methods for the Microbiological Examination of Foods , 1992 .

[52]  S. Radu,et al.  Inhibitory effect of oxalic acid on bacterial spoilage of raw chilled chicken. , 2006, Journal of food protection.

[53]  J. Samelis,et al.  The spoilage microflora of cured, cooked turkey breasts prepared commercially with or without smoking. , 2000, International journal of food microbiology.

[54]  M. Dermiki,et al.  Shelf-life extension and quality attributes of the whey cheese “Myzithra Kalathaki” using modified atmosphere packaging , 2008 .