Recovery of gene function by gene duplication in Saccharomyces cerevisiae

A prototroph revertant (Rev9) selected from an ATCase− mutant of the URA2 gene containing three nonsense mutations was shown to contain two ATCase coding sequences. We cloned both ATCase coding areas to show that the duplicated locus (dl9) was the only functional one. Its size corresponded roughly to the second half of the URA2 wild‐type gene. Sequence analysis of the 5′ end of dl9 indicated that this duplicated sequence was inserted within the intergenic region close to the MRS3 gene and was transcribed from an unknown promoter divergently from the MRS3 gene. The event leading to the revertant strain Rev9 included a rearrangement that increased the size of chromosome X by about 60 kb. In agreement with such a rearrangement, recombination was undetectable in the vicinity of the locus dl9. Genetic mapping confirms that the MRS3 gene is 2 cM distal to the URA2 gene on the right arm of chromosome X.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  C. Ball,et al.  Genetic and physical maps of Saccharomyces cerevisiae. , 1997, Nature.

[3]  R. Mortimer,et al.  Genetic and physical maps of Saccharomyces cerevisiae, edition 11 , 1992, Yeast.

[4]  S. Liebman,et al.  Rearrangements occurring adjacent to a single Ty1 yeast retrotransposon in the presence and absence of full-length Ty1 transcription. , 1992, Genetics.

[5]  R. Schweyen,et al.  MRS3 and MRS4, two suppressors of mtRNA splicing defects in yeast, are new members of the mitochondrial carrier family. , 1991, Journal of molecular biology.

[6]  R. Losson,et al.  cis- and trans-acting regulatory elements of the yeast URA3 promoter , 1990, Molecular and cellular biology.

[7]  J. Souciet,et al.  Studies on transcription of the yeast URA2 gene. , 1990, FEMS microbiology letters.

[8]  F. Watt,et al.  DNA amplification is rare in normal human cells. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Souciet,et al.  Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex. , 1989, Gene.

[10]  G. Wahl,et al.  Recent progress in understanding mechanisms of mammalian DNA amplification , 1989, Cell.

[11]  R. W. Davis,et al.  Separation of large DNA molecules by contour-clamped homogeneous electric fields. , 1986, Science.

[12]  K. Struhl.  Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[13]  M. Olson,et al.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. , 1984, Nucleic acids research.

[14]  J. Devereux,et al.  A comprehensive set of sequence analysis programs for the VAX , 1984, Nucleic Acids Res..

[15]  E. Scolnick,et al.  ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae , 1983, Nature.

[16]  M. Chevallier,et al.  Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. , 1980, Gene.

[17]  R. W. Davis,et al.  Replacement of chromosome segments with altered DNA sequences constructed in vitro. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[18]  G. Fink,et al.  Transformation of yeast. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[19]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[20]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[21]  M. Ciriacy.  Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae: I. Isolation and genetic analysis of adh mutants , 1975 .

[22]  C. Waldron,et al.  Effect of growth rate on the amounts of ribosomal and transfer ribonucleic acids in yeast , 1975, Journal of bacteriology.

[23]  M Mandel,et al.  Calcium-dependent bacteriophage DNA infection. , 1970, Journal of molecular biology.

[24]  D. Helinski,et al.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[25]  M. Grenson,et al.  The biosynthesis of carbamoyl phosphate in Saccharomyces cerevisiae. , 1965, Journal of general microbiology.

[26]  N. Glansdorff.  TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12. , 1965, Genetics.

[27]  C. B. Bridges,et al.  THE BAR "GENE" A DUPLICATION. , 1936, Science.

[28]  B. Dujon,et al.  Construction of a cosmid contig and of an EcoRI restriction map of yeast chromosome X. , 1994, DNA sequence : the journal of DNA sequencing and mapping.

[29]  G. Fink,et al.  Eviction and transplacement of mutant genes in yeast. , 1983, Methods in enzymology.

[30]  R. Mortimer,et al.  Genetic Mapping in Saccharomyces cerevisiae , 1981 .

[31]  R. Mortimer,et al.  Genetic mapping in Saccharomyces. , 1966, Genetics.

[32]  B. D. B. CLEWELLt,et al.  SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN ESCHERICHIA COLI: PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM* , 2022 .