Synthesis and Characterization of Molecularly Imprinted Polymer as Adsorbent for D-arabinitol

D-arabinitol is a metabolite product typically of several Candida species that are pathogenic and potentially used for candidiasis markers. In this study, a molecularly imprinted polymers (MIPs) of D-arabinitol has been synthesized and functions as D-arabinitol adsorbent. MIPs will functioned as an adsorbent after removing the template. The resulting polymers was characterized by FTIR, LC-MS and its binding efficiency tested with a batch binding assay. The results of FTIR analysis showed that the MIPs produced contained both monomers and templates. The template removal process was successful enough to see a significant reduction in the D-arabinitol area (no template detected) with LC-MS. The results of the batch binding assay indicated that MIPs has a binding capacity value (Q) of 1.075 mg/g whereas NIPs has a value (Q) of 0.131 mg/g. The results showed that the MIPs produced was potentially used as an adsorbent of D-arabinitol.

[1]  K. Row,et al.  Recent Applications of Molecularly Imprinted Polymers (MIPs) on Micro-extraction Techniques , 2018 .

[2]  V. Prachayasittikul,et al.  Effects of Polymerization Methods and Functional Monomers on Curcumin Imprinted Polymer Properties , 2014 .

[3]  N. Adhoum,et al.  Preparation and application of a molecularly imprinted polymer for determination of glibenclamide residues , 2013, Acta pharmaceutica.

[4]  J. Kałużna-Czaplińska,et al.  The level of arabinitol in autistic children after probiotic therapy. , 2012, Nutrition.

[5]  G. Karimi,et al.  Clozapine recognition via molecularly imprinted polymers; bulk polymerization versus precipitation method , 2011 .

[6]  Giuseppe Vasapollo,et al.  Molecularly Imprinted Polymers: Present and Future Prospective , 2011, International journal of molecular sciences.

[7]  Bao-jiao Gao,et al.  Constructing chiral caves and efficiently separating enantiomers of glutamic acid with novel surface-imprinting technique. , 2011, Journal of chromatography. A.

[8]  M. Syczewska,et al.  Determination of urinary D-/L-arabinitol ratios as a biomarker for invasive candidiasis in children with cardiac diseases. , 2010, Journal of medical microbiology.

[9]  A. Telefoncu,et al.  Noncovalently galactose imprinted polymer for the recognition of different saccharides. , 2009, Talanta.

[10]  J. Coates Interpretation of Infrared Spectra, A Practical Approach , 2006 .

[11]  S. Piletsky,et al.  Comparison of thin-layer and bulk MIPs synthesized by photoinitiated in situ crosslinking polymerization from the same reaction mixtures , 2005 .

[12]  A. Ellepola,et al.  Laboratory diagnosis of invasive candidiasis. , 2005, Journal of microbiology.

[13]  W. Shaw,et al.  Increased urinary excretion of analogs of Krebs cycle metabolites and arabinose in two brothers with autistic features. , 1995, Clinical chemistry.