Control of dihydrofolate reductase messenger ribonucleic acid production

We used methotrexate-resistant mouse cells in which dihydrofolate reductase levels are approximately 500 times normal to study the effect of growth stimulation on dihydrofolate reductase gene expression. As a result of growth stimulation, the relative rate of dihydrofolate reductase protein synthesis increased threefold, reaching a maximum between 25 and 30 h after stimulation. The relative rate of dihydrofolate reductase messenger ribonucleic acid production (i.e., the appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm) increased threefold after growth stimulation and was accompanied by a corresponding increase in the relative steady-state level of dihydrofolate reductase ribonucleic acid in the nucleus. However, the increase in the nuclear level of dihydrofolate reductase ribonucleic acid was not accompanied by a significant increase in the relative rate of transcription of the dihydrofolate reductase genes. These data indicated that the relative rate of appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm depends on the relative stability of the dihydrofolate reductase ribonucleic acid sequences in the nucleus and is not dependent on the relative rate of transcription of the dihydrofolate reductase genes.

[1]  T. Gudewicz,et al.  The effect of polyoma virus, serum factors, and dibutyryl cyclic AMP on dihydrofolate reductase synthesis, and the entry of quiescent cells into S phase , 1981, Journal of cellular physiology.

[2]  R. Palmiter,et al.  Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids , 1981, Nature.

[3]  James E. Darnell,et al.  Transcriptional control in the production of liver-specific mRNAs , 1981, Cell.

[4]  M. Harper,et al.  Molecular basis of methotrexate resistance , 1981 .

[5]  E. Ziff.  Transcription and RNA processing by the DNA tumour viruses , 1980, Nature.

[6]  L. Johnson,et al.  Cell cycle regulation of dihydrofolate reductase mRNA metabolism in mouse fibroblasts. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Rogers,et al.  Two mRNAs can be produced from a single immunoglobulin μ gene by alternative RNA processing pathways , 1980, Cell.

[8]  David Baltimore,et al.  Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3′ ends , 1980, Cell.

[9]  J. Rogers,et al.  Two mRNAs with different 3′ ends encode membrane-bound and secreted forms of immunoglobulin μ chain , 1980, Cell.

[10]  K. Lowenhaupt,et al.  Synthesis and turnover of globin mRNA in murine erythroleukemia cells induced with hemin. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Darnell,et al.  Production of mRNA in chinese hamster cells: Relationship of the rate of synthesis to the cytoplasmic concentration of nine specific mRNA sequences , 1979, Cell.

[12]  R. Matusik,et al.  Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression , 1979, Cell.

[13]  R. Britten,et al.  Regulation of gene expression: possible role of repetitive sequences. , 1979, Science.

[14]  E. Pfendt,et al.  Polyoma virus and cyclic AMP-mediated control of dihydrofolate reductase mRNA abundance in methotrexate-resistant mouse fibroblasts. , 1979, The Journal of biological chemistry.

[15]  J. Nevins,et al.  Steps in the processing of Ad2 mRNA: Poly(A)+ Nuclear sequences are conserved and poly(A) addition precedes splicing , 1978, Cell.

[16]  L. Johnson,et al.  Regulation of dihydrofolate reductase gene expression in mouse fibroblasts during the transition from the resting to growing state , 1978, Journal of cellular physiology.

[17]  Stanley N Cohen,et al.  Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase , 1978, Nature.

[18]  B. Wold,et al.  Sea urchin embryo mRNA sequences expressed in the nuclear RNA of adult tissues , 1978, Cell.

[19]  J. Bertino,et al.  Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. , 1978, The Journal of biological chemistry.

[20]  G. Spinelli,et al.  Synthesis of histone messenger RNA of HeLa cells during the cell cycle , 1977, Cell.

[21]  P. Sharp,et al.  Spliced segments at the 5′ terminus of adenovirus 2 late mRNA* , 1977, Proceedings of the National Academy of Sciences.

[22]  K. Yamamoto,et al.  Glucocorticoid-stimulated accumulation of mouse mammary tumor virus RNA: increased rate of synthesis of viral RNA. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[23]  P. Williams,et al.  Purification and characterization of covalently closed replicative intermediates of ColEl DNA from Escherichia coli. , 1977, Biochemistry.

[24]  R. Strohman,et al.  Messenger RNA for myosin polypeptides: Isolation from single myogenic cell cultures , 1977, Cell.

[25]  S. Levy,et al.  Biosynthesis and stability of globin mRNA in cultured erythroleukemic friend cells , 1976, Cell.

[26]  R. Schimke,et al.  Synthesis and degradation of folate reductase in sensitive and methotrexate-resistant lines of S-180 cells. , 1976, The Journal of biological chemistry.

[27]  B. Daneholt.  Transcription in polytene chromosomes , 1975, Cell.

[28]  R. Palmiter,et al.  Rapid inactivation of ovalbumin messenger ribonucleic acid after acute withdrawal of estrogen. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[29]  D. R. Dubbs,et al.  Induction of dihydrofolate reductase activity by SV40 and polyoma virus. , 1966, Cancer research.

[30]  M. Hakala,et al.  Relation of folic acid reductase to amethopterin resistance in cultured mammalian cells. , 1961, The Journal of biological chemistry.