Correlation between aminoglycoside resistance profiles and DNA hybridization of clinical isolates

DNA hybridization data and aminoglycoside resistance profiles (AGRPs) were determined for 4,088 clinical isolates from three studies (United States, Belgium, and Argentina). The correlation between susceptibility profiles and hybridization results was determined with nine DNA probes. For each of the seven aminoglycoside resistance profiles which we were able to test, the data suggested at least two distinct genes could encode enzymes which lead to identical resistance profiles. Furthermore, the DNA hybridization data showed that individual strains carried up to six unique aminoglycoside resistance genes. DNA hybridization revealed interesting differences in the frequencies of these genes by organism and by country.

[1]  D. Gerding,et al.  More than one DNA sequence encodes the 2''-O-adenylyltransferase phenotype , 1987, Antimicrobial Agents and Chemotherapy.

[2]  P. Courvalin,et al.  Detection of apramycin resistant Enterobacteriaceae in hospital isolates. , 1989, FEMS microbiology letters.

[3]  H. Chung,et al.  Comparison of aminoglycoside resistance patterns in Japan, Formosa, and Korea, Chile, and the United States , 1985, Antimicrobial Agents and Chemotherapy.

[4]  D. Reeves,et al.  Resistance of bacterial pathogens to four aminoglycosides and six other antibacterials and prevalence of aminoglycoside modifying enzymes, in 20 UK centres. , 1988, The Journal of antimicrobial chemotherapy.

[5]  A. Feinberg,et al.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. , 1983, Analytical biochemistry.

[6]  P. O'Hara,et al.  Development of a DNA probe from the deoxyribonucleotide sequence of a 3-N-aminoglycoside acetyltransferase [AAC(3)-I] resistance gene , 1989, Antimicrobial Agents and Chemotherapy.

[7]  N. Barg.  Construction of a probe for the aminoglycoside 3-V-acetyltransferase gene and detection of the gene among endemic clinical isolates , 1988, Antimicrobial Agents and Chemotherapy.

[8]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[9]  C. M. Joyce,et al.  Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[10]  E. Collatz,et al.  Primary structure of an aminoglycoside 6'-N-acetyltransferase AAC(6')-4, fused in vivo with the signal peptide of the Tn3-encoded beta-lactamase , 1987, Journal of bacteriology.

[11]  C. Carlier,et al.  Gene homogeneity for aminoglycoside-modifying enzymes in gram-positive cocci , 1990, Antimicrobial Agents and Chemotherapy.

[12]  P. Rather,et al.  Characterization of the chromosomal aac(6')-Ic gene from Serratia marcescens , 1992, Antimicrobial Agents and Chemotherapy.

[13]  N. Legakis,et al.  Survey of aminoglycoside resistance patterns. , 1986, Chemioterapia : international journal of the Mediterranean Society of Chemotherapy.

[14]  T. Gootz,et al.  Development ofTwoDNA Probes forDifferentiating theStructural GenesofSubclasses IandII oftheAminoglycoside-Modifying Enzyme3'-Aminoglycoside Phosphotransferase , 1985 .

[15]  K. Shaw,et al.  Isolation, characterization, and DNA sequence analysis of an AAC(6')-II gene from Pseudomonas aeruginosa , 1989, Antimicrobial Agents and Chemotherapy.

[16]  P. Courvalin,et al.  Transferable amikacin resistance in Acinetobacter spp. due to a new type of 3'-aminoglycoside phosphotransferase , 1988, Antimicrobial Agents and Chemotherapy.

[17]  P. M. Terry,et al.  Use of plasmid analysis and determination of aminoglycoside-modifying enzymes to characterize isolates from an outbreak of methicillin-resistant Staphylococcus aureus , 1989, Journal of clinical microbiology.

[18]  R. Gaynes,et al.  Isolation, characterization, and cloning of a plasmid-borne gene encoding a phosphotransferase that confers high-level amikacin resistance in enteric bacilli , 1988, Antimicrobial Agents and Chemotherapy.

[19]  T. Gootz,et al.  Development of a DNA probe for the structural gene of the 2"-O-adenyltransferase aminoglycoside-modifying enzyme. , 1984, Journal of Infectious Diseases.

[20]  D. Vapnek,et al.  Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenyltransferase , 1985 .

[21]  G. Bongaerts,et al.  A simple method for the identification of aminoglycoside-modifying enzymes. , 1984, The Journal of antimicrobial chemotherapy.

[22]  T. Gootz,et al.  Development of two DNA probes for differentiating the structural genes of subclasses I and II of the aminoglycoside-modifying enzyme 3'-aminoglycoside phosphotransferase , 1985, Antimicrobial Agents and Chemotherapy.

[23]  F. Tenover,et al.  Cloning and sequencing of a gene encoding an aminoglycoside 6'-N-acetyltransferase from an R factor of Citrobacter diversus , 1988, Journal of bacteriology.

[24]  S. Mitsuhashi.  Proposal for a Rational Nomenclature for Phenotype, Genotype and Aminoglycoside-Aminocyclitol Modifying Enzymes , 1975 .

[25]  D. Reeves,et al.  AAC(1): a new aminoglycoside-acetylating enzyme modifying the Cl aminogroup of apramycin. , 1987, The Journal of antimicrobial chemotherapy.

[26]  M. Syvanen,et al.  Intercontinental spread of a new antibiotic resistance gene on an epidemic plasmid. , 1985, Science.

[27]  J. Vliegenthart,et al.  Nucleotide sequence of the aacC2 gene, a gentamicin resistance determinant involved in a hospital epidemic of multiply resistant members of the family Enterobacteriaceae , 1989, Antimicrobial Agents and Chemotherapy.

[28]  P. Bennett,et al.  Cloning and characterization of an AAC(6') gene from Serratia marcescens. , 1988, The Journal of antimicrobial chemotherapy.