mRNA-electroporated mature dendritic cells retain transgene expression, phenotypical properties and stimulatory capacity after cryopreservation

[1]  C. van Broeckhoven,et al.  Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells. , 2001, Blood.

[2]  G. Schuler,et al.  Human dendritic cells transfected with either RNA or DNA encoding influenza matrix protein M1 differ in their ability to stimulate cytotoxic T lymphocytes , 2000, Gene Therapy.

[3]  G. Schuler,et al.  A method for the production of cryopreserved aliquots of antigen-preloaded, mature dendritic cells ready for clinical use. , 2000, Journal of immunological methods.

[4]  W. Scherbaum,et al.  Response of human monocyte‐derived dendritic cells to immunostimulatory DNA , 2000, European journal of immunology.

[5]  D. Nixon,et al.  Requirement of Mature Dendritic Cells for Efficient Activation of Influenza A-Specific Memory CD8+ T Cells1 , 2000, The Journal of Immunology.

[6]  E. Gilboa,et al.  Induction of Cytotoxic T Lymphocytes With Dendritic Cells Transfected With Human Papillomavirus E6 and E7 RNA: Implications for Cervical Cancer Immunotherapy , 2000, Journal of immunotherapy.

[7]  P. Martiat,et al.  Freezing of dendritic cells, generated from cryopreserved leukaphereses, does not influence their ability to induce antigen-specific immune responses or functionally react to maturation stimuli. , 2000, Journal of immunological methods.

[8]  E. Gilboa.  The makings of a tumor rejection antigen. , 1999, Immunity.

[9]  C. Broeckhoven,et al.  Nonviral transfection of distinct types of human dendritic cells: high-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells , 1998, Gene Therapy.

[10]  E. Gilboa,et al.  Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA , 1998, Nature Biotechnology.

[11]  R. Steinman,et al.  Dendritic cells and the control of immunity , 1998, Nature.

[12]  M. Baba,et al.  A Cryopreservation Method of Human Peripheral Blood Mononuclear Cells for Efficient Production of Dendritic Cells , 1997, Scandinavian journal of immunology.

[13]  R. Steinman,et al.  Proliferating dendritic cell progenitors in human blood , 1994, The Journal of experimental medicine.