Preparation of chitosan-coated nanoliposomes for improving the mucoadhesive property of curcumin using the ethanol injection method.

Chitosan-coated curcumin nanoliposomes (CS-Cur-NLs) were fabricated by the ethanol injection method (EIM), and their physicochemical properties were compared with the properties of those fabricated by the dry thin film method (DTFM). The mean size and zeta potential of CS-Cur-NLs gradually increased with CS concentration. The encapsulation efficiency of Cur-NLs prepared by EIM was 54.70%, which was significantly improved compared to that (42.60%) of Cur-NLs prepared by DTFM. Further improvement of encapsulation efficiency was attained (up to 64.93%) by EIM with 0.1% CS coating. The mucoadhesive property of Cur-NLs improved from 33.60 to 56.47% with CS coating. The results indicate that the encapsulated curcumin will show prolonged adsorption in the gastrointestinal tract because of higher mucoadhesion. Thus, EIM can be considered to be effective for food-grade delivery carriers with higher encapsulation efficiency and absence of harmful solvents. EIM-generated CS-Cur-NLs showed higher bioavailability, with enhanced high mucoadhesive property, storage stability, and encapsulation efficiency.

[1]  A. Bangham,et al.  Diffusion of univalent ions across the lamellae of swollen phospholipids. , 1965, Journal of molecular biology.

[2]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[3]  Miquel Pons,et al.  Liposomes obtained by the ethanol injection method , 1993 .

[4]  Y. Kawashima,et al.  Mucoadhesion of polymer-coated liposomes to rat intestine in vitro. , 1994, Chemical & pharmaceutical bulletin.

[5]  N. Peppas,et al.  Hydrogels as mucoadhesive and bioadhesive materials: a review. , 1996, Biomaterials.

[6]  J. Lowe,et al.  Chitosan as a nasal delivery system: the effect of chitosan solutions on in vitro and in vivo mucociliary transport rates in human turbinates and volunteers. , 1997, Journal of pharmaceutical sciences.

[7]  A. Perkins,et al.  Evaluation of the clearance characteristics of bioadhesive systems in humans. , 1999, International journal of pharmaceutics.

[8]  J. Robinson,et al.  Bioadhesive-based dosage forms: the next generation. , 2000, Journal of pharmaceutical sciences.

[9]  M. Brandl.  Liposomes as drug carriers: a technological approach. , 2001, Biotechnology annual review.

[10]  N. Škalko-Basnet,et al.  Mucoadhesive chitosan-coated liposomes: characteristics and stability , 2001, Journal of microencapsulation.

[11]  J. B. Park,et al.  Preparation of alginate beads for floating drug delivery system: effects of CO(2) gas-forming agents. , 2002, International journal of pharmaceutics.

[12]  Tsutomu Suzuki,et al.  Chitosan capsules for colon-specific drug delivery: enhanced localization of 5-aminosalicylic acid in the large intestine accelerates healing of TNBS-induced colitis in rats. , 2002, Journal of controlled release : official journal of the Controlled Release Society.

[13]  Ananth Annapragada,et al.  Controlled targeting of liposomal doxorubicin via the folate receptor in vitro. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[14]  B. Mukherjee,et al.  Plant Medicines of Indian Origin for Wound Healing Activity: A Review , 2003, The international journal of lower extremity wounds.

[15]  B. Lokesh,et al.  Studies on spice principles as antioxidants in the inhibition of lipid peroxidation of rat liver microsomes , 1992, Molecular and Cellular Biochemistry.

[16]  B. Timmermann,et al.  The effect of turmeric extracts on inflammatory mediator production. , 2005, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[17]  Hoo-Kyun Choi,et al.  Mucoadhesive microspheres prepared by interpolymer complexation and solvent diffusion method. , 2005, International journal of pharmaceutics.

[18]  J. Smart.  The basics and underlying mechanisms of mucoadhesion. , 2005, Advanced drug delivery reviews.

[19]  C. Allen,et al.  Hybrid films from blends of chitosan and egg phosphatidylcholine for localized delivery of paclitaxel. , 2005, Journal of pharmaceutical sciences.

[20]  Rama Bansil,et al.  Mucin structure, aggregation, physiological functions and biomedical applications , 2006 .

[21]  P. Colombo,et al.  Formation of self-organized nanoparticles by lecithin/chitosan ionic interaction. , 2006, International journal of pharmaceutics.

[22]  Y. Maitani,et al.  Cationic liposome (DC-Chol/DOPE=1:2) and a modified ethanol injection method to prepare liposomes, increased gene expression. , 2007, International journal of pharmaceutics.

[23]  R. Kurzrock,et al.  Liposomal curcumin with and without oxaliplatin: effects on cell growth, apoptosis, and angiogenesis in colorectal cancer , 2007, Molecular Cancer Therapeutics.

[24]  B. Aggarwal,et al.  Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins. , 2008, Cancer letters.

[25]  Kwangmeyung Kim,et al.  Antitumor efficacy of cisplatin-loaded glycol chitosan nanoparticles in tumor-bearing mice. , 2008, Journal of controlled release : official journal of the Controlled Release Society.

[26]  D. Mcclements,et al.  Impact of surfactant properties on oxidative stability of beta-carotene encapsulated within solid lipid nanoparticles. , 2009, Journal of agricultural and food chemistry.

[27]  Makoto Takahashi,et al.  Evaluation of an oral carrier system in rats: bioavailability and antioxidant properties of liposome-encapsulated curcumin. , 2009, Journal of agricultural and food chemistry.

[28]  G. Feldmann,et al.  Systemic Administration of Polymeric Nanoparticle-Encapsulated Curcumin (NanoCurc) Blocks Tumor Growth and Metastases in Preclinical Models of Pancreatic Cancer , 2010, Molecular Cancer Therapeutics.

[29]  N. Škalko-Basnet,et al.  Mucoadhesive liposomal delivery systems: the choice of coating material , 2011, Drug development and industrial pharmacy.

[30]  M. Kępczyński,et al.  Interaction of curcumin with lipid monolayers and liposomal bilayers. , 2011, Colloids and surfaces. B, Biointerfaces.

[31]  Rupesh Kumar Basniwal,et al.  Curcumin nanoparticles: preparation, characterization, and antimicrobial study. , 2011, Journal of agricultural and food chemistry.

[32]  Fengyi Zhang,et al.  PLGA nanoparticles improve the oral bioavailability of curcumin in rats: characterizations and mechanisms. , 2011, Journal of agricultural and food chemistry.

[33]  M. Paoletti,et al.  Current views on fungal chitin/chitosan, human chitinases, food preservation, glucans, pectins and inulin: A tribute to Henri Braconnot, precursor of the carbohydrate polymers science, on the chitin bicentennial , 2012 .

[34]  Murali M. Yallapu,et al.  Curcumin nanoformulations: a future nanomedicine for cancer. , 2012, Drug discovery today.

[35]  H. Park,et al.  Size-controlled self-aggregated N-acyl chitosan nanoparticles as a vitamin C carrier , 2012 .

[36]  Q. Zhong,et al.  Enhanced dispersibility and bioactivity of curcumin by encapsulation in casein nanocapsules. , 2013, Journal of agricultural and food chemistry.

[37]  C. Duyckaerts,et al.  Curcumin-conjugated nanoliposomes with high affinity for Aβ deposits: possible applications to Alzheimer disease. , 2013, Nanomedicine : nanotechnology, biology, and medicine.

[38]  X. Chen,et al.  Preparation and oral bioavailability study of curcuminoid-loaded microemulsion. , 2013, Journal of agricultural and food chemistry.