Epithelial and mesenchymal cell interactions with extracellular matrix material in vitro.

Epidermal organogenesis (thyroid gland, salivary gland, feather, hair, skin, thymus gland, tooth, etc.) generally follows a basic rule; epithelium exhibits well-documented interdependence with adjacent mesenchyme for a specific path of development (Grobstein, 1967, for review). Koch (1967) demonstrated in rodent embryos that isolates of incisor epithelial and mesenchymal tissue, separated by a millipore filter, continued to develop. When homotypic tissues were placed in juxtaposition to the filter, no evidence of continued differentiation was observed. Isolated cervical loop tissues of tooth germs from mammalian embryos have been shown to develop into an entire tooth in vitro (Slavkin & Bavetta, 1968 a ; Kollar & Baird, 1969). Our laboratory recently reported that isolated tissue preparations (Slavkin & Bavetta, 1968 a ) or cell suspensions (Slavkin, Beierle & Bavetta, 1968) of epithelial and mesenchymal cells from the embryonic cervical loop, in recombination on the chick chorioallantoic membrane (CAM), reconstituted and developed into a tooth germ.

[1]  M. Karnovsky.  SIMPLE METHODS FOR "STAINING WITH LEAD" AT HIGH pH IN ELECTRON MICROSCOPY , 1961, The Journal of biophysical and biochemical cytology.

[2]  E. Kollar,et al.  The influence of the dental papilla on the development of tooth shape in embryonic mouse tooth germs. , 1969, Journal of embryology and experimental morphology.

[3]  J. A. Freeman,et al.  TECHNICAL MODIFICATIONS IN MARAGLAS EMBEDDING , 1963, The Journal of cell biology.

[4]  W. E. Koch,et al.  In vitro differentiation of tooth rudiments of embryonic mice. I. Transfilter interaction of embryonic incisor tissues. , 1967, The Journal of experimental zoology.

[5]  Paul Weiss,et al.  Guiding principles in cell locomotion and cell aggregation , 1961 .

[6]  John Tyler Bonner,et al.  Morphogenesis , 1965, Cell.

[7]  P. Weiss.  Perspectives in the Field of Morphogenesis , 1950, The Quarterly Review of Biology.

[8]  B. D. Davis,et al.  The specificity of cell surfaces , 1967 .

[9]  C. Grobstein,et al.  SOURCE OF COLLAGEN AT EPITHELIOMESENCHYMAL INTERFACES DURING INDUCTIVE INTERACTION. , 1965, Developmental biology.

[10]  P. Weiss,et al.  Shape and Movement of Mesenchyme Cells as Functions of the Physical Structure of the Medium: Contributions to a Quantitative Morphology. , 1952, Proceedings of the National Academy of Sciences of the United States of America.

[11]  C Grobstein,et al.  Mechanisms of organogenetic tissue interaction. , 1967, National Cancer Institute monograph.

[12]  K. Dan.  Cyto-embryology of echinoderms and amphibia. , 1960, International review of cytology.

[13]  C. Grobstein,et al.  Localization of glucosamine-incorporating materials at epithelial surfaces during salivary epithelio-mesenchymal interaction in vitro. , 1966, Developmental biology.

[14]  T. Gustafson,et al.  THE CELLULAR BASIS OF MORPHOGENESIS AND SEA URCHIN DEVELOPMENT. , 1963, International review of cytology.

[15]  E. Pannese.  Observations on the ultrastructure of the enamel organ. III. Internal and external enamel epithelia. , 1962, Journal of Ultrastructure Research.

[16]  L. Weiss.  The cell periphery, metastasis and other contact phenomena , 1967 .

[17]  A. Moscona.  Analysis of cell recombinations in experimental synthesis of tissues in vitro , 1962 .

[18]  A. Moscona.  CHAPTER 14 – Recombination of Dissociated Cells and the Development of Cell Aggregates , 1965 .

[19]  H. Slavkin,et al.  Odontogenesis: Cell–Cell Interactions in vitro , 1968, Nature.

[20]  H. Slavkin,et al.  Odontogenic Epithelial-Mesenchymal Interactions in Vitro , 1968, Journal of dentistry research.

[21]  E. Reith,et al.  The early stage of amelogenesis as observed in molar teeth of young rats. , 1967, Journal of ultrastructure research.

[22]  H. Slavkin,et al.  Morphogenetic expressions during odontogenesis: a tool in developmental biology. , 1968, Clinical orthopaedics and related research.